Diversity, vitality and activities of intestinal lactic acid bacteria and bifidobacteria assessed by molecular approaches

Vaughan, Elaine E.; Heilig, Hans G.H.J.; BENAMOR, K; DEVOS, W

doi:10.1016/j.femsre.2005.04.009

Cited by 70 publications

(41 citation statements)

References 99 publications

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“…The great majority (up to about 80%) of these bacterial species have not been cultured. These numbers have mainly been assessed by molecular methods, counting both dead and live cells (34, 35). The advantage of our study is the possibility to explore the potential interplay between intestinal microbiota and human metabolism.…”

Section: Discussionmentioning

confidence: 99%

Higher blood glucose level associated with body mass index and gut microbiota in elderly people

Sepp¹,

Kolk²,

Lõivukene³

et al. 2014

Microbial Ecology in Health & Disease

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BackgroundSome dominant bacterial divisions of the intestines have been linked to metabolic diseases such as overweight and diabetes.ObjectiveA pilot study aimed to evaluate the relations between the culturable intestinal bacteria with body mass index (BMI) and some principal cellular and metabolic markers of blood in people older than 65.DesignAltogether 38 generally healthy elderly people were recruited: ambulatory (n=19) and orthopedic surgery (n=19). Questionnaires on general health, anthropometric measurements, routine clinical and laboratory data, and quantitative composition of cultivable gut microbiota were performed.ResultsBlood glucose level was positively correlated with BMI (r=0.402; p=0.014). Higher blood glucose level had negative correlation with relative share of intestinal anaerobic bacteria such as bacteroides (r=−0.434; p=0.0076) and gram-positive anaerobic cocci (r=−0.364; p=0.027). In contrast, the relative share of bifidobacteria (r=0.383; p=0.019) and staphylococci (r=0.433; p=0.008) was positively correlated to blood glucose level. In elderly people, a higher blood glucose concentration was predicted by the reduction of the anaerobes’ proportion (adj. sex, age, and BMI R2=0.192, p=0.028) and that of Bacteroides sp. (adj. R2=0.309, p=0.016).ConclusionA tight interplay between increased BMI, level of blood glucose, and the reduced proportion of cultivable bacteroides is taking place in the gut microbiota of elderly people.

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Section: Discussionmentioning

confidence: 99%

Higher blood glucose level associated with body mass index and gut microbiota in elderly people

Sepp¹,

Kolk²,

Lõivukene³

et al. 2014

Microbial Ecology in Health & Disease

View full text Add to dashboard Cite

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“…Using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene amplicons, the same group later showed that the colonic mucosal microbiota and faecal microbiota were different, and the colonic mucosal microbiota was likely dependent on host factors [46]. Meanwhile fluorescent hybridization of probes for 16S rRNA genes was being applied to determine species identities, numbers, and proportions of intestinal bacteria [47, 48], exemplified by the studies of Dore, Blaut and colleagues [49, 50]. These analyses highlighted extensive inter-individual variation at phylotypes level (among northern Europeans) [49], and some correlations of microbiota with age, gender, and geographic origin but which varied between countries [50].…”

Section: The Normal Microbiota Of Humans and Animalsmentioning

confidence: 99%

Probiotic Bacteria Influence the Composition and Function of the Intestinal Microbiota

O’Toole

Cooney

2008

Interdisciplinary Perspectives on Infectious Diseases

146

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Probiotics have a range of proposed health benefits for the consumer, which may include modulating the levels of beneficial elements in the microbiota. Recent investigations using molecular approaches have revealed a human intestinal microbiota comprising over 1000 phylotypes. Mechanisms whereby probiotics impact on the intestinal microbiota include competition for substrates, direct antagonism by inhibitory substances, competitive exclusion, and potentially host-mediated effects such as improved barrier function and altered immune response. We now have the microbial inventories and genetic blueprints to begin tackling intestinal microbial ecology at an unprecedented level of detail, aided by the understanding that dietary components may be utilized differentially by individual phylotypes. Controlled intervention studies in humans, utilizing latest molecular technologies, are required to consolidate evidence for bacterial species that impact on the microbiota. Mechanistic insights should be provided by metabolomics and other analytical techniques for small molecules. Rigorous characterization of interactions between the diet, microbiota, and probiotic bacteria will provide new opportunities for modulating the microbiota towards improving human health.

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“…In this report we have investigated the expression of class I heat shock genes in Lactobacillus plantarum , a lactic acid bacterium widely distributed in different environmental niches including the mammalian gastrointestinal tract [12]. Indeed, L. plantarum is considered an important component of the intestinal microbiota of healthy individuals and for its properties is considered a probiotic LAB [1,12].…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, L. plantarum is considered an important component of the intestinal microbiota of healthy individuals and for its properties is considered a probiotic LAB [1,12]. It is also widely distributed in most fermented products of animal and plant origin either as starter or as NSLAB [13], and more recently it has been used for nutraceutical productions [14].…”

Section: Introductionmentioning

confidence: 99%

CcpA affects expression of the groESL and dnaK operons in Lactobacillus plantarum

et al. 2006

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BackgroundLactic acid bacteria (LAB) are widely used in food industry and their growth performance is important for the quality of the fermented product. During industrial processes changes in temperature may represent an environmental stress to be overcome by starters and non-starters LAB. Studies on adaptation to heat shock have shown the involvement of the chaperon system-proteins in various Gram-positive bacteria. The corresponding operons, namely the dnaK and groESL operons, are controlled by a negative mechanism involving the HrcA repressor protein binding to the cis acting element CIRCE.ResultsWe studied adaptation to heat shock in the lactic acid bacterium Lactobacillus plantarum. The LM3-2 strain, carrying a null mutation in the ccpA gene, encoding the catabolite control protein A (CcpA), showed a lower percent of survival to high temperature with respect to the LM3 wild type strain. Among proteins differentially expressed in the two strains, the GroES chaperon was more abundant in the wild type strain compared to the mutant strain under standard growth conditions. Transcriptional studies showed that class I heat shock operons were differentially expressed upon heat shock in both strains. Indeed, the dnaK and groESL operons were induced about two times more in the LM3 strain compared to the LM3-2 strain. Analysis of the regulatory region of the two operons showed the presence of cre sequences, putative binding sites for the CcpA protein.ConclusionThe L. plantarum dnaK and groESL operons are characterized by the presence of the cis acting sequence CIRCE in the promoter region, suggesting a negative regulation by the HrcA/CIRCE system, which is a common type of control among the class I heat shock operons of Gram-positive bacteria. We found an additional system of regulation, based on a positive control exerted by the CcpA protein, which would interact with cre sequences present in the regulatory region of the dnaK and groESL operons. The absence of the CcpA protein results in a lower induction of the chaperon coding operons, with a consequent lower percent of survival of the LM3-2 mutant strain population with respect to the wild type when challenged with a heat insult.

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Diversity, vitality and activities of intestinal lactic acid bacteria and bifidobacteria assessed by molecular approaches

Cited by 70 publications

References 99 publications

Higher blood glucose level associated with body mass index and gut microbiota in elderly people

Higher blood glucose level associated with body mass index and gut microbiota in elderly people

Probiotic Bacteria Influence the Composition and Function of the Intestinal Microbiota

CcpA affects expression of the groESL and dnaK operons in Lactobacillus plantarum

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