Diverting intracellular trafficking of<i>Salmonella</i>to the lysosome through activation of the late endocytic Rab7 by intracellular delivery of muramyl dipeptide

Mukherjee, Konark; Parashuraman, Seetharaman; Krishnamurthy, G.; Majumdar, J.; Yadav, Ashok K.; Kumar, Ravi; Basu, Sandip K.; Mukhopadhyay, Asok

doi:10.1242/jcs.00034

Cited by 30 publications

(32 citation statements)

References 43 publications

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“…Based on the present data, it can be proposed that an opposite effect would be seen with these drugs with respect to vertical transmission of HIV-1. In contrast, our data raise the interesting possibility that altering the endocytic Rab content by intervention in appropriate signal transduction events may offer a new strategy for modulating the targeting of HIV-1 to a degradative compartment (69). Additional studies are needed to define the validity of this approach.…”

Section: Discussionmentioning

confidence: 85%

Rab5 and Rab7, but Not ARF6, Govern the Early Events of HIV-1 Infection in Polarized Human Placental Cells

Vidricaire

Tremblay

2005

The Journal of Immunology

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Trophoblasts, the structural cells of the placenta, are thought to play a determinant role in in utero HIV type 1 (HIV-1) transmission. We have accumulated evidence suggesting that HIV-1 infection of these cells is associated with uptake by an unusual clathrin/caveolae-independent endocytic pathway and that endocytosis is followed by trafficking through multiple organelles. Furthermore, part of this trafficking involves the transit of HIV-1 from transferrin-negative to EEA1 and transferrin-positive endosomes, suggesting a merger from nonclassical to classical endocytic pathways in these cells. In the present article, the relationship between the presence of HIV-1 within specific endosomes and infection was studied. We demonstrate that viral infection is virtually lost when endosome inhibitors are added shortly after exposure to HIV-1. Thus, contrary to what is seen in CD4+ T lymphocytes, the initial presence of HIV-1 within the endosomes is mandatory for infection to take place. Importantly, this process is independent of the viral envelope proteins gp120 and gp41. The Rab family of small GTPases coordinates the vesicular transport between the different endocytic organelles. Experiments performed with various expression vectors indicated that HIV-1 infection in polarized trophoblasts relies on Rab5 and Rab7 without the contribution of Arf6 or Rab11. Furthermore, we conclude that Rab5 drives movements from raft-rich region to early endosomes, and this transit is required for subsequently reaching late endosomes via Rab7. This complex trafficking is mandatory for HIV-1 infection to proceed in human polarized trophoblasts.

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Section: Discussionmentioning

confidence: 85%

Rab5 and Rab7, but Not ARF6, Govern the Early Events of HIV-1 Infection in Polarized Human Placental Cells

Vidricaire

Tremblay

2005

The Journal of Immunology

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“…This late stage in SCV maturation is not fully understood, yet. On the one hand, studies using primary macrophages demonstrated that SCVs do fuse with the lysosomal compartment (Mills and Finlay, 1998;Garvis et al, 2001;Mukherjee et al, 2002). On the other hand, however, studies indicated that despite expressing the phagosomal marker LAMP1, SCVs do segregate and avoid fusion with mature lysosomes to establish a replicative niche for the Salmonella.…”

Section: Discussionmentioning

confidence: 99%

The late endosomal adaptor p14 is a macrophage host defense factor against Salmonella Typhimurium infection

Taub

Nairz

Hilber

et al. 2012

Journal of Cell Science

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SummaryThe outcome of an infection depends on the balance between host resistance and bacterial virulence. Here, we show that the late endosomal adaptor p14 (also known as LAMTOR2) is one of the components for cellular host defense against the intracellular pathogen Salmonella enterica serovar Typhimurium. During Salmonella infection, the complex of p14 and MP1 is required for the accurately timed transport of Salmonella through the endolysosomal system. Loss of p14 opens a time window that allows Salmonella to populate a replication niche, in which early and late antimicrobial effector systems, comprising NADPH phagocytic oxidase and inducible nitric oxide synthase, respectively, are inappropriately activated. Thus, p14 supports the accurate transport of Salmonella through the endolysosomal system, thereby limiting bacterial replication in both, professional phagocytes and in non-phagocytic cells in vitro, and helps mice to successfully battle Salmonella infection in vivo.

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“…Pathogenic bacteria have developed two major strategies to counteract macrophage killing after phagocytosis, either by escaping from the endocytic vacuoles and inducing cell death (14,25,50) or by resisting the antimicrobial environment of the phagolysosome (32,41,43). We previously reported that the reference AIEC strain LF82 did not induce the death of infected macrophages despite the high replication of bacteria observed within large vacuoles (19).…”

Section: Discussionmentioning

confidence: 99%

HtrA Stress Protein Is Involved in Intramacrophagic Replication of Adherent and Invasive Escherichia coli Strain LF82 Isolated from a Patient with Crohn's Disease

Bringer¹,

Barnich²,

Glasser³

et al. 2005

Infect Immun

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Adherent and invasive Escherichia coli (AIEC) bacteria isolated from Crohn's disease patients are able to greatly replicate within macrophages without escaping from the phagosome and without inducing macrophage death. In the present study, evidence is provided that in AIEC strain LF82 the htrA gene encoding the stress protein HtrA is essential for intracellular replication within J774-A1 macrophages. Deletion of the htrA gene in strain LF82 induced increased sensitivity of the isogenic mutant to oxidative stress caused by hydrogen peroxide and a reduced rate of growth in an acid and nutrient-poor medium partly reproducing the microenvironment of the phagosome. In vitro experiments using an LF82 htrA gene promoter fusion with the lacZ gene revealed a 38-fold activation of the promoter in AIEC LF82 intramacrophagic bacteria. The CpxRA twocomponent signaling pathway was not involved in this activation. In addition, the activation of the LF82 htrA gene promoter was not observed in the nonpathogenic E. coli K-12 intramacrophagic bacteria, indicating that the AIEC LF82 genetic background is crucial for induction of htrA gene transcription during phagocytosis.

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Diverting intracellular trafficking ofSalmonellato the lysosome through activation of the late endocytic Rab7 by intracellular delivery of muramyl dipeptide

Cited by 30 publications

References 43 publications

Rab5 and Rab7, but Not ARF6, Govern the Early Events of HIV-1 Infection in Polarized Human Placental Cells

Rab5 and Rab7, but Not ARF6, Govern the Early Events of HIV-1 Infection in Polarized Human Placental Cells

The late endosomal adaptor p14 is a macrophage host defense factor against Salmonella Typhimurium infection

HtrA Stress Protein Is Involved in Intramacrophagic Replication of Adherent and Invasive Escherichia coli Strain LF82 Isolated from a Patient with Crohn's Disease

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