2018
DOI: 10.1002/cm.21490
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DivIVA concentrates mycobacterial cell envelope assembly for initiation and stabilization of polar growth

Abstract: In many model organisms, diffuse patterning of cell wall peptidoglycan synthesis by the actin homolog MreB enables the bacteria to maintain their characteristic rod shape. In Caulobacter crescentus and Escherichia coli, MreB is also required to sculpt this morphology de novo. Mycobacteria are rod-shaped but expand their cell wall from discrete polar or subpolar zones. In this genus, the tropomyosin-like protein DivIVA is required for the maintenance of cell morphology. DivIVA has also been proposed to direct p… Show more

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Cited by 46 publications
(53 citation statements)
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“…Extended incubation of M. smegmatis (~48 hr) with the peptidoglycan-degrading enzyme lysozyme substantially decreases colony-forming units ( Kanetsuna, 1980 ). We have also shown that spheroplasts generated by combined glycine and lysozyme treatment lack peptidoglycan ( Melzer et al, 2018 ). Together these data indicate that the enzyme is able to access and damage peptidoglycan in intact cells.…”
Section: Resultsmentioning
confidence: 96%
“…Extended incubation of M. smegmatis (~48 hr) with the peptidoglycan-degrading enzyme lysozyme substantially decreases colony-forming units ( Kanetsuna, 1980 ). We have also shown that spheroplasts generated by combined glycine and lysozyme treatment lack peptidoglycan ( Melzer et al, 2018 ). Together these data indicate that the enzyme is able to access and damage peptidoglycan in intact cells.…”
Section: Resultsmentioning
confidence: 96%
“…In Mycobacterium species (which, unlike Firmicutes, undergo apical growth [121]), DivIVA is essential (122)(123)(124) and localizes to the septum, cell poles, and branching sites (124)(125)(126)(127). Overproduction of DivIVA leads to polar bulging and branching in Mycobacterium smegmatis (125), a fast-growing cousin of M. tuberculosis, where DivIVA dictates the site of peptidoglycan insertion (128,129). An indirect link between DivIVA and Z-ring assembly was revealed by the elucidation of direct interactions between DivIVA and a cell wall synthesis protein (CwsA) and FtsZinteracting FtsI/PBP3 (130-133) ( Table S1B).…”
Section: Multiple Functions Of Diviva In Actinobacteriamentioning
confidence: 99%
“…In B. subtilis and E. coli , actin homologs like MreB organize RIFs (Oswald et al, 2016; Strahl et al, 2014) and direct peptidoglycan synthesis along the lateral cell surface (Daniel & Errington, 2003; Iwai et al, 2002; Shi et al, 2018; Zhao et al, 2017). While MreB is absent in mycobacteria, the essential tropomyosin-like protein DivIVA concentrates cell wall assembly at the poles (Daniel & Errington, 2003; Iwai et al, 2002; Melzer et al, 2018; Shi et al, 2018; Zhao et al, 2017). DivIVA depletion results in deformation and rounding of mycobacterial cells (Kang et al, 2008).…”
Section: Mainmentioning
confidence: 99%