dl-3-n-Butylphthalide protects rat bone marrow stem cells against hydrogen peroxide-induced cell death through antioxidation and activation of PI3K-Akt pathway

Sun, Bo; Feng, Ming; Tian, Xiangyang; Lu, Xiaowei; Zhang, Yunyun; Ke, Xianjin; Huang, Susu; Cao, Jingjing; Xin-sheng, Ding

doi:10.1016/j.neulet.2012.04.003

Cited by 33 publications

(27 citation statements)

References 28 publications

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“…NBP as a natural antioxidant could provide protection to the ischemic brain via PI3K/Akt pathway [26]. Moreover, the improvement of energy pump and the subsidence of oxidative injury upon NBP treatment might also mitigate against apoptosis in such injuries [27].…”

Section: Discussionmentioning

confidence: 99%

DL-3-n-Butylphthalide, an Anti-Oxidant Agent, Prevents Neurological Deficits and Cerebral Injury Following Stroke per Functional Analysis, Magnetic Resonance Imaging and Histological Assessment

Zhang¹,

Yu²,

Wáng³

et al. 2012

CNR

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DL-3-n-Butylphthalide (NBP) is a synthetic compound based on L-3-n-Butylphthalide which was isolated from seeds of Apium graveolens. The present study aims at evaluating the outcome of NBP given prior to and after the onset of ischemic stroke in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY). Stroke was induced by the middle cerebral artery occlusion (MCAO) in SHR and WKY. For pre-treatment, NBP was administered to SHR and WKY daily for two months prior to MCAO. For post-treatment, NBP was given daily for seven consecutive days after MCAO. Seven days post-surgery, rats were tested for the presence of neurological deficits. Magnetic resonance imaging (MRI) and 2,3,5-triphenyltetrazolium chloride (TTC) staining were employed to calculate the infarct volume. The cerebral cortex and corpus striatum in the ischemic penumbra area were examined microscopically for pathological changes. In SHR, NBP pre- and post-treatment significantly lowered neurological deficit scores, reduced infarct volume, and minimized pathological changes in the penumbra area when compared to oil-vehicle treated controls. In WKY, these beneficial effects were observed only in the post-treatment group. The beneficial effects of NBP post-treatment were greater in WKY than in SHR. Results indicated that NBP could exert both preventive and therapeutic effects on ischemic stroke in SHR, but only exerted therapeutic effect in WKY.

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Section: Discussionmentioning

confidence: 99%

DL-3-n-Butylphthalide, an Anti-Oxidant Agent, Prevents Neurological Deficits and Cerebral Injury Following Stroke per Functional Analysis, Magnetic Resonance Imaging and Histological Assessment

Zhang¹,

Yu²,

Wáng³

et al. 2012

CNR

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“…Treatment with long-term administration of NBP on stroke model indicated a significant decrease of malondialdehyde (MDA) levels in brain tissues of rats, suggesting that NBP counteracts excitotoxicity and restores the balance by enhancement of antioxidant response [27]. Another study on bone marrow of rat confirmed those findings and further suggested that dl-NBP reduces hydrogen peroxide- (H 2 O 2 -) induced accumulation of reactive oxygen species (ROS) [25]. These findings combined demonstrate that NBP's multiple antioxidant effects can be decisive in the treatment outcome of ischemic stroke patients and might constitute an important field for further therapeutic options.…”

Section: Nbp and Ischemic Strokementioning

confidence: 98%

“…An experimental study showed an increase in levels of Bcl-2 and hypoxia-inducible factor 1 alpha (HIF-1 α ) coupled with a decrease of caspase-3 expression [22] after administration of dl-NBP [24]. Another study suggested a new antiapoptotic approach of dl-NBP through activation of phosphatidylinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway [25] and possible regulation of c-Jun N-terminal kinase (JNK) and p38 mitogen-actives protein kinases (MAPK) activation [22]. These studies demonstrated that dl-NBP could be a good candidate for prevention of further cellular death in the ischemic penumbra.…”

Section: Nbp and Ischemic Strokementioning

confidence: 99%

A Review of Recent Advances in Neuroprotective Potential of 3-N-Butylphthalide and Its Derivatives

Abdoulaye

Guo

2016

BioMed Research International

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The research of alternative treatment for ischemic stroke and degenerative diseases has always been a priority in neurology. 3-N-Butylphthalide (NBP), a family of compounds initially isolated from the seeds of Apium graveolens Linn., has shown significant neuroprotective effects. Previous extensive studies have demonstrated that NBP promotes a better poststroke outcome and exerts a multitargeted action on several mechanisms, from oxidative stress to mitochondrial dysfunction to apoptosis to inflammation. Additionally, recent findings on several neurological disorders have shown that NBP's beneficial effects extend beyond the management of stroke. However, despite the increasing number of studies toward a better understanding and the rapid advances made in therapeutic options, to date, dl-3-N-butylphthalide, a synthetic variation of l-3-N-butylphthalide, remains the only clinically approved anti-ischemic agent in China, stressing the difficulties for a viable and effective transition from experimental to clinical practice. Events indicate that NBP, due to its multitargeted effect and the adaptability of its basic structure, can be an important game changer and a precursor to a whole new therapeutic approach to several neurological conditions. The present review discusses recent advances pertaining to the neuroprotective mechanisms of NBP-derived compounds and the possibility of their clinical implementation in the management of various neurological conditions.

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“…Western blotting analysis was performed as previously described (29). Briefly, at the end of the treatment period, cells were collected and lysed with cold lysis buffer.…”

Section: Detection Of Superoxide Dismutase (Sod)mentioning

confidence: 99%

Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells

Wang

Nianhong

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to be used for the treatment of delayed union, nonunion or persistent bone defects in MSC-based cell therapy. However, implantation of BMSCs into the fracture site is confronted with apoptosis on account of harsh conditions and oxidative stress. In the present study, the anti-apoptotic effects of berberine (BBR) on BMSCs subjected to hydrogen peroxide (H 2 O 2 ) are investigated, and the potential underlying mechanisms are explored. Oxidative injury was induced by exposure to H 2 O 2 , and cell viability was assessed using a cell counting kit-8 assay. The apoptosis of BMSCs was measured by Hoechst 33258 and Annexin V-fluorescein isothiocyanate/propidium iodide assay. Reactive oxygen species staining and superoxide dismutase (SOD) assay were applied to assess the anti-oxidative effect of BBR. Finally, western blot was performed to measure the expression levels of phosphorylated (p)-Akt, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax) and cleaved caspase-3. In the present study, it was identified that BBR remarkably attenuated H 2 O 2 -induced apoptotic cell death via quenching ROS production and increasing SOD activity. Further studies indicated that BBR can reduce apoptosis by upregulating the expression level of p-Akt and Bcl-2, and downregulating the expression levels of Bax and cleaved caspase-3. Taken together, the results of the present study demonstrate that pretreatment with BBR could alleviate H 2 O 2 -induced apoptosis in rat BMSCs in vitro.

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dl-3-n-Butylphthalide protects rat bone marrow stem cells against hydrogen peroxide-induced cell death through antioxidation and activation of PI3K-Akt pathway

Cited by 33 publications

References 28 publications

DL-3-n-Butylphthalide, an Anti-Oxidant Agent, Prevents Neurological Deficits and Cerebral Injury Following Stroke per Functional Analysis, Magnetic Resonance Imaging and Histological Assessment

DL-3-n-Butylphthalide, an Anti-Oxidant Agent, Prevents Neurological Deficits and Cerebral Injury Following Stroke per Functional Analysis, Magnetic Resonance Imaging and Histological Assessment

A Review of Recent Advances in Neuroprotective Potential of 3-N-Butylphthalide and Its Derivatives

Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells

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