2024
DOI: 10.1364/ao.546044
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DL-CSPF: deep-learning-based cell segmentation with a physical framework for digital holographic microscopy

Zhuoshi Li,
Haojie Gu,
Linpeng Lu
et al.

Abstract: Digital holographic microscopy (DHM) offers label-free, full-field imaging of live-cell samples by capturing optical path differences to produce quantitative phase images. Accurate cell segmentation from phase images is crucial for long-term quantitative analysis. However, complicated cellular states (e.g., cell adhesion, proliferation, and apoptosis) and imaging conditions (e.g., noise and magnification) pose significant challenge to the accuracy of cell segmentation. Here, … Show more

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