DL-leucine transport in aSaccharomyces cerevisiae mutant resistant to quaternary ammonium salts

Obłąk, Ewa; Lachowicz, T; Witek, S.

doi:10.1007/bf02816365

Cited by 9 publications

(10 citation statements)

References 1 publication

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“…In the yeast Saccharomyces cerevisiae, reduced susceptibility to benzalkonium chloride has been associated with loss of a cytoplasmic membrane, active-transport leucine pump (209)(210). The possibility arises that the quaternary ammonium biocides might also utilize such transport processes in order to gain access to bacterial cells and that deletion/repression might be associated with resistance.…”

Section: Reduced Susceptibility To Biocides Associated With Chromosommentioning

confidence: 99%

Potential Impact of Increased Use of Biocides in Consumer Products on Prevalence of Antibiotic Resistance

2003

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There has recently been much controversy surrounding the increased use of antibacterial substances in a wide range of consumer products and the possibility that, as with antibiotics, indiscriminate use of biocides might contribute to the overall pattern of susceptibility in the general environment and in the clinic. Such speculation, based on the isolation of resistant mutants from in vitro monoculture experiments, is not reflected by an emergence of biocide-resistant strains in vivo. This review provides a broad coverage of the biocide and resistance literature and evaluates the potential risks, perceived from such laboratory monoculture experiments, against evidence gathered over 50 years of field studies. An explanation for the continued effectiveness of broad-spectrum biocidal agents against the decline in efficacy of therapeutic agents is provided based on the fitness costs of resistance and the ubiquity of naturally occurring substances that possess antibacterial effect. While we conclude from this review of the literature that the incorporation of antibacterial agents into a widening sphere of personal products has had little or no impact on the patterns of microbial susceptibility observed in the environment, the associated risks remain finite. The use of such products should therefore be associated with a clear demonstration of added value either to consumer health or to the product life. Hygienic products should therefore be targeted to applications for which the risks have been established

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Section: Reduced Susceptibility To Biocides Associated With Chromosommentioning

confidence: 99%

Potential Impact of Increased Use of Biocides in Consumer Products on Prevalence of Antibiotic Resistance

2003

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“…They anchor to the lipid regions of the cell membrane by the hydrophobic aliphatic carbon chain. However, their positively charged nitrogen head remains at the surface, thus interfering with its function of proton extrusion by plasma membrane H + -ATPase, especially in the transport of amino acids into the cell [27,28,34,35].…”

Section: Discussionmentioning

confidence: 99%

“…Sensitivity to QAS also depends on the biosynthetic and respiratory proficiencies of the yeast. Therefore, auxotrophic and respiratory-deficient mutants are more sensitive than isogenic prototrophic and respiratory-proficient strains [27][28][29][30][31][32]. In previous investigations, it was also found that the inhibitory activity of QAS is pH dependent: the activity of QAS is higher at pH 8 than at pH 6 [27,28,33].…”

Section: Introductionmentioning

confidence: 99%

“…In previous investigations, it was also found that the inhibitory activity of QAS is pH dependent: the activity of QAS is higher at pH 8 than at pH 6 [27,28,33]. Moreover, the compounds inhibit specific and general amino-acid transport into yeast cells [34,35] and the activity of the plasma membrane and mitochondrial ATPases [27,28,34]. In order to more precisely locate the target of QAS action in yeast cells, we analysed the morphological changes occurring under the influence of the tested compound.…”

Section: Introductionmentioning

confidence: 99%

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The physiological and morphological phenotype of a yeast mutant resistant to the quaternary ammonium salt N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride

Obłąk

Gamian²,

Adamski

et al. 2010

Cellular and Molecular Biology Letters

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Abstract:We investigated the action of the quaternary ammonium salt (QAS) called IM (N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride) on Saccharomyces cerevisiae yeast cells. Changes in the yeast cell ultrastructure were confirmed by electron microscopy. We treated resistant mutant cells with QAS, and confirmed destruction of the mutant cytoplasm, an increase in the thickness of the cell wall, separation of the cell wall from the cytoplasm, and the accumulation of numerous lipid droplets. We also observed a relatively high production of lipids in the cells of the parental wild-type strain Σ1278b and in its IM-resistant (IM R ) mutant in the presence of the QAS. The IM R mutant showed increased sensitivity to CaCl 2 and SDS, and resistance to ethidium bromide, chloramphenicol, erythromycin and osmotic shock. It also tolerated growth at low pH. We suggest that the resistance to IM could be connected with the level of permeability of the cell membrane because the IM R mutant was sensitive to this compound in vivo in the presence of SDS and guanidine hydrochloride, which cause increased permeability of the cell plasma membrane.

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“…• C. The cells were subjected to survival tests carried out according to a standard procedure [18] in a liquid medium supplemented with a definite amount of the particular dopant dissolved in DMF. The quantities of CTP-Sn and CTP-Pb fed into the investigated system are presented in Table 1.…”

Section: Cells and Growth Conditionsmentioning

confidence: 99%

Effect of tin and lead chlorotriphenyl analogues on selected living cells

Boniewska-Bernacka

Man

Słota

et al. 2010

J Biochem & Molecular Tox

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Three kinds of living cells, human embryonic kidney cells, Saccharomyces cerevisiae, and Escherichia coli, were tested for their sensitivity to chlorotriphenyltin and chlorotriphenyllead. The tin compound proved definitely more toxic than the lead derivative, particularly in the case of the human embryonic kidney cells devoid of any protective cell wall. Electron paramagnetic resonance (EPR) comparative studies carried out by using a natural model liposome system (egg yolk lecithin) confirmed considerable changes within the lipid bilayer upon doping by the aforementioned additives, which may be crucial to the mechanism of the observed cell cleavage. The individual dopants revealed diverse impact upon the membrane's condition, chlorotriphenyltin distinctly fluidized the lipid system, whereas chlorotriphenyllead stiffened the medium within the membrane. A theoretical approach concerning such different behaviors of studied tin and lead analogues because of their high toxicity in living cells has been presented.

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DL-leucine transport in aSaccharomyces cerevisiae mutant resistant to quaternary ammonium salts

Cited by 9 publications

References 1 publication

Potential Impact of Increased Use of Biocides in Consumer Products on Prevalence of Antibiotic Resistance

Potential Impact of Increased Use of Biocides in Consumer Products on Prevalence of Antibiotic Resistance

The physiological and morphological phenotype of a yeast mutant resistant to the quaternary ammonium salt N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride

Effect of tin and lead chlorotriphenyl analogues on selected living cells

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