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Jaiti, Fatima; Benlhabib, Ouafae; Sharma, H. C.; Jaafari, Samir El; Hadrami, I. El

doi:10.1023/a:1006399512217

Cited by 16 publications

(1 citation statement)

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“…Ovary co-culture has had a stimulatory effect on microspore regeneration in early wheat studies (Datta and Wenzel 1987) and has been used to improve embryo response in durum wheat anther culture (Aiti et al 1999). There are no other reports of its use in current bread wheat anther culture protocols although the use of ovary conditioned medium has been reported (Soriano et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat (Triticum aestivum L.)

Broughton

2008

Plant Cell Tiss Organ Cult

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A simple anther culture protocol for Australian spring wheat cultivars was developed using ovary co-culture. The inclusion of ovaries in the induction medium significantly increased the production of embryo-like structures (ELS), green and albino plants in two spring wheat cultivars tested. When five ovaries were added to the induction medium, the mean number of ELS per spike increased from 7.6 to 50.1 and green plants per spike increased from 0.6 to 8.9. The addition of 10 ovaries, however, did not further increase the production of ELS or green plants. The growth regulator combination of 2,4-D and KIN was compared with IAA and BA. There were no significant differences in the numbers of ELS or green plants although significantly fewer albino plants were produced with IAA and BA. Eight additional cultivars were screened using the protocol with either 5 or 10 ovaries in the induction medium. Green plants were obtained from nine varieties at frequencies ranging from 0.3 to 33.0 green plants per spike. Regenerant plants at maturity exhibited chromosome fertility rates in different cultivars ranging from 15% to 100%.

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Section: Introductionmentioning

confidence: 99%