An attempt was made to evaluate nondestructively the degradation of thermally aged 2.25Cr-1Mo steel by electrical resistivity measurement. Artificial aging was performed to simulate the microstructural changes in 2.25Cr-1Mo steel arising from long time exposure at 540 C. Microstructural parameter (amount of dissolved Mo in the matrix), mechanical property (ductile-brittle transition temperature) and electrical resistivity were measured to investigate the relationship among these parameters. Both the amount of dissolved Mo and the electrical resistivity decreased rapidly in the initial 1000 hours of aging and then changed little thereafter. On the other hand, the ductile-brittle transition temperature (DBTT) increased rapidly in the initial stage of aging and then saturated afterward. Electrical resistivity was found to have linear correlation with the amount of dissolved Mo and DBTT, respectively. Electrical resistivity was suggested as a potential nondestructive evaluation parameter for assessing DBTT of the thermally degraded 2.25Cr-1Mo steel.