DMC1: A meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression

Bishop, Douglas K.; Park, Demian; Xu, Liuzhong; Kleckner, Nancy

doi:10.1016/0092-8674(92)90446-j

Cited by 1,124 publications

(1,208 citation statements)

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“…Previous work provided evidence that DMC1-independent recombination can also occur without RAD54 over-expression during meiosis (Bishop et al 1992;Rockmill & Roeder 1994;Rockmill et al 1995). Again, DMC1-independent recombination depends largely on the function of DMC1's relative, RAD51 (Rockmill et al 1995;Dresser et al 1997;Schwacha & Kleckner 1997;.…”

Section: High Copy Numbers Of Rec114 Block Recombination Before Dsbsmentioning

confidence: 99%

“…Filters were hybridized to a probe that detects a 1 kb region HIS4 proximal to the downstream PstI site as described in Bishop et al (1992). The position of the full length PstI fragment, representing sequences that do not contain a meiotic DSB, is indicated as`P'.…”

Section: High Copy Numbers Of Rec114 Block Recombination Before Dsbsmentioning

confidence: 99%

“…This conclusion is supported by the observation that wild-type cells with high copy numbers of REC114 produce inviable spores, because pre-DSB blocks in recombination have this effect (Malone & Esposito 1981;Malone et al 1991). We anticipated isolating dmc1 arrest suppressors which block recombination before the DSB stage because earlier studies showed that mutations in early recombination genes prevent dmc1 arrest (Bishop et al 1992;D.K.B., unpublished data). Null mutations in rec114 prevent DSB formation (Pittman et al 1993).…”

Section: High Copy Numbers Of Rec114 Block Recombination Before Dsbsmentioning

confidence: 99%

“…While DMC1-independent meiotic recombination is inef®cient in the SK-1 strain background used here, two types of perturbations can increase the ef®ciency of this type of recombination. First, when dmc1 mutants are returned to mitotic growth after induction of DSBs, mutant cells retain full viability (Bishop et al 1992). Physical analysis has shown that DSBs are very rapidly repaired after the reintroduction of nutrients that support mitotic growth (Schwacha & Kleckner 1997;Zenvirth et al 1997).…”

Section: Rad54-mediated Repair Of Dsbs In Dmc1 Mutants Requires Disrumentioning

confidence: 99%

“…Mutations in checkpoint genes, when present in otherwise wild-type strains, do not reduce the ef®-ciency of DSB formation and have relatively modest effects on interhomologue recombination and viable meiotic product formation (Lydall et al 1996;Grushcow et al 1999). Another mechanism through which dmc1 arrest can be overcome is mutation of one of the many`early' recombination genes required for DSB formation (Bishop et al 1992; D.K.B., unpublished observations; R.E. Malone, personal communication).…”

Section: Introductionmentioning

confidence: 99%

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High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1‐independent DSB repair pathway

et al. 1999

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Background: DMC1, the meiosis-speci®c eukaryotic homologue of bacterial recA, is required for completion of meiotic recombination and cell cycle progression past prophase. In a dmc1 mutant, double strand break recombination intermediates accumulate and cells arrest in prophase. We isolated genes which, when present at high copy numbers, suppress the meiotic arrest phenotype conferred by dmc1 mutations.

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Section: High Copy Numbers Of Rec114 Block Recombination Before Dsbsmentioning

confidence: 99%

Section: High Copy Numbers Of Rec114 Block Recombination Before Dsbsmentioning

confidence: 99%

Section: High Copy Numbers Of Rec114 Block Recombination Before Dsbsmentioning

confidence: 99%

Section: Rad54-mediated Repair Of Dsbs In Dmc1 Mutants Requires Disrumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1‐independent DSB repair pathway

et al. 1999

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The differentiated and conserved roles of Swi5‐Sfr1 in homologous recombination

2017

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Homologous recombination (HR) is the process whereby two DNA molecules that share high sequence similarity are able to recombine to generate hybrid DNA molecules. Throughout evolution, the ability of HR to identify highly similar DNA sequences has been adopted for numerous biological phenomena including DNA repair, meiosis, telomere maintenance, ribosomal DNA amplification and immunological diversity. Although Rad51 and Dmc1 are the key proteins that promote HR in mitotic and meiotic cells, respectively, accessory proteins that allow Rad51 and Dmc1 to effectively fulfil their functions have been identified in all examined model systems. In this Review, we discuss the roles of the highly conserved Swi5‐Sfr1 accessory complex in yeast, mice and humans, and explore similarities and differences between these species.

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Insights into the identification and evolutionary conservation of key genes in the transcriptional circuits of meiosis initiation and commitment in budding yeast

Das,

Chaudhary,

Ali

et al. 2023

FEBS Open Bio

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Initiation of meiosis in budding yeast does not commit the cells for meiosis. Thus, two distinct signaling cascades may differentially regulate meiosis initiation and commitment in budding yeast. To distinguish between the role of these signaling cascades, we reconstructed protein–protein interaction networks and gene regulatory networks with upregulated genes in meiosis initiation and commitment. Analyzing the integrated networks, we identified four master regulators (MRs) [Ume6p, Msn2p, Met31p, Ino2p], three transcription factors (TFs), and 279 target genes (TGs) unique for meiosis initiation, and three MRs [Ndt80p, Aro80p, Rds2p], 11 TFs, and 948 TGs unique for meiosis commitment. Functional enrichment analysis of these distinct members from the transcriptional cascades for meiosis initiation and commitment revealed that nutritional cues rewire gene expression for initiating meiosis and chromosomal recombination commits cells to meiosis. As meiotic chromosomal recombination is highly conserved in eukaryotes, we compared the evolutionary rate of unique members in the transcriptional cascade of two meiotic phases of Saccharomyces cerevisiae with members of the phylum Ascomycota, revealing that the transcriptional cascade governing chromosomal recombination during meiosis commitment has experienced greater purifying selection pressure (P value = 0.0013, 0.0382, 0.0448, 0.0369, 0.02967, 0.04937, 0.03046, 0.03357 and < 0.00001 for Ashbya gossypii, Yarrowia lipolytica, Debaryomyces hansenii, Aspergillus fumigatus, Neurospora crassa, Kluyveromyces lactis, Schizosaccharomyces pombe, Schizosaccharomyces cryophilus, and Schizosaccharomyces octosporus, respectively). This study demarcates crucial players driving meiosis initiation and commitment and demonstrates their differential rate of evolution in budding yeast.

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DMC1: A meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression

Cited by 1,124 publications

References 75 publications

High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1‐independent DSB repair pathway

High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1‐independent DSB repair pathway

The differentiated and conserved roles of Swi5‐Sfr1 in homologous recombination

Insights into the identification and evolutionary conservation of key genes in the transcriptional circuits of meiosis initiation and commitment in budding yeast

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