DMD-based compact SIM system with hexagonal-lattice-structured illumination

Abstract

In this study, we developed a novel, compact, and efficient structured illumination microscopy (SIM) system, to our best knowledge. A binary hexagonal lattice pattern was designed and implemented on a digital micromirror device (DMD), resulting in a projection-based structured-light generation. By leveraging the combination of the high-speed switching capability of the DMD with a high-speed CMOS camera, the system can capture 1024×1024 pixels images at a 200 fps frame rate when provided with sufficient illumination power. The loading of the hexagonal lattice pattern reduces the number of images required for reconstruction to seven, and by utilizing the DMD modulating characteristics on the illumination path, there is no need to use bulky mechanical structures for phase shifting. We designed a compact system with 110mm×150mm×170mm dimensions that displayed a 1.61 resolution enhancement for fluorescent particle and biological sample imaging.

Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane

“…Although recent developments demonstrate, for example, scanner-based systems 70 or fiber optic configurations 71 to achieve fast TIRF-SIM, these systems are more complex to synchronize or less versatile, respectively, to achieve multimodal multi-color high-speed imaging. Recently, digital mirror devices (DMDs) were introduced as a promising alternative to SLMs for high-speed SIM applications 72 – 76 , but robust multi-color applications with more than two colors are still challenging. Furthermore, new developments in image denoising will provide more opportunities to apply fast SIM in biological samples 54 , 77 .…”