DMRT1 expression during gonadal differentiation and spermatogenesis in the rainbow trout, Oncorhynchus mykiss

Sexual dimorphic expression of DMRT1 and Sox9a during gonadal differentiation and hormone‐induced sex reversal in the teleost fish Nile tilapia (Oreochromis niloticus)

“…Evidence of the male-specific expression of DMRT1 was also obtained in humans, a reptile species and a frog species (Raymond et al, 1999;Smith et al, 1999;Kettlewell et al, 2000;Moniot et al, 2000;Shibata et al, 2002). In teleost fish, several reports indicate that DMRT1 expression is related to the development of the testes (Guan et al, 2000;Marchand et al, 2000;He et al, 2003). The present study indicates clearly, for the first time in fish, that tDMRT1 is expressed in the germcell-surrounding cells and medullarycell-mass cells, which differentiate into the efferent duct during testicular differentiation, irrespective of the genetic sex.…”

“…In a study using semiquantitative RT-PCR in trout, estrogen-induced XY sex reversal was associated with a reduction in DMRT1 expression in the gonads (Marchand et al, 2000). In tilapia, the Northern analysis has shown that DMRT1 is expressed in XX testes, just as in XY testes (Guan et al, 2000).…”

“…In chickens, aromatase inhibitor-treated ZW embryos showed sex reversal, accompanied by DMRT1 expression (Smith et al, 2003). In teleost fish also, DMRT1 has been linked to both natural sex change from female to male, and to sex hormone-dependent sex reversal (He et al, 2003;Guan et al, 2000;Marchand et al, 2000). These findings strongly suggest that DMRT1 homologs are involved in testicular differentiation in the process of normal sex development and sex reversal in nonmammalian vertebrates.…”

“…However, recent studies indicate that some downstream gene products of sex determination genes are functionally similar in diverse species. One of these genes, the doublesex/mab-3 conserved domain (DM) domain-containing gene (DMRT1; doublesex/mab-3 related transcription factor-1), has been found in mammals, a bird species, a reptile species, a frog species, teleosts, nematodes, and flies (Shen and Hodgkin, 1988;Coschigano and Wensink, 1993;Raymond et al, 1998Raymond et al, , 1999Smith et al, 1999;De Grandi et al, 2000;Kettlewell et al, 2000;Guan et al, 2000;Marchand et al, 2000;Shibata et al, 2002). The DM domain, a zinc fingerlike DNA-binding motif (Erdman and Burtis, 1993;Raymond et al, 1998), was first identified in the sexual regulatory proteins Drosophila doublesex and C. elegans mab-3 and is widely conserved among metazoans.…”

Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers

“…Pask et al, 2003;Raymond et al, 1999;Smith et al, 1999), birds (Nanda et al, 1999;Shan et al, 2000;Shetty et al, 2002;Smith et al, 1999), reptiles (Kettlewell et al, 2000;Smith et al, 1999) and fish (Brunner et al, 2001;Huang et al, 2005;Marchand et al, 2000). In each case except the mouse, DMRT1 is expressed at a higher level in potential male genital ridge than in potential female genital ridge before gonad differentiation.…”