DMSA-camSPECT/US fusion imaging of children’s kidneys – Proof of feasibility

Gühne, Falk; Weigel, Friederike; Kühnel, Christian; Seifert, Philipp; Freesmeyer, Martin

doi:10.1055/a-1073-6875

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…This probe also has attachment points for customizable multi-purpose needle-guided systems. Its curved shape allows deeper penetration into the body, making it suitable for visualizing organs like the liver, with special fusion imaging possibilities for the kidneys [17]. Finally, one endocavitary probe was considered, the E8CS (GE Healthcare, Waukesha, WI, USA) for abdominal or even gynaecological applications.…”

Section: Probesmentioning

confidence: 99%

Visualization of Effectiveness: The Use of a Set of Colored Cleaning Wipes for Visible Disinfection of Ultrasound Probes

Kühnel,

Gühne

2024

Hygiene

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The German health authorities’ guidelines for medical devices in 2012 highlighted the importance of cleaning ultrasound probes, emphasizing their validation and reliability. In addition to automated and validated options, alternative manual methods such as wipe disinfection have gained traction due to their independence from additional hardware. The study examines the effectiveness of a manual cleaning process using wipes, addressing concerns raised by the Robert Koch Institute regarding the lack of validation for wipe disinfection of semi-critical devices. The EQUINOS colored wipe disinfection kit identified wetting gaps in all cleanings across four probes tested. The results indicate significant challenges in ensuring complete surface wetting, particularly in complex device parts such as clip-on areas and fixtures for additional biopsy attachments, suggesting that manual methods alone may not adequately mitigate the risk of infection transmission (p value < 0.0001). The study concludes that while manual disinfection methods are a commonly used alternative to automated reprocessing, there is a critical need for enhanced training and potentially the development of more effective manual disinfection techniques or colored wipes to ensure patient safety and compliance with healthcare hygiene standards.

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Section: Probesmentioning

confidence: 99%

Visualization of Effectiveness: The Use of a Set of Colored Cleaning Wipes for Visible Disinfection of Ultrasound Probes

Kühnel,

Gühne

2024

Hygiene

Self Cite

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“…As noted above, conventional renal tracers such as DMSA and GHA also have some renal accumulations and therefore are used in conjunction with SPECT to image functional renal mass 34 , 35 . Our results showed a very modest retention of DMSA in organs including the lung and the kidney (longer organ retention than MAG3: Figure S5 ).…”

Section: Discussionmentioning

confidence: 99%

Targeted design of a recombinant tracer for SPECT renal imaging

et al. 2021

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Rationale: A robust radiopharmaceutical has high uptake in the target and low retention in non-target tissues. However, traditional tracers for renal imaging that chemically chelate 99m Tc are excreted through the renal route with transient resident time in the kidney. Following a rational design approach, we constructed a protein-based radiotracer, designated PBT-Fc, to sequentially bind tubular neonatal Fc-receptor and subsequently proximal tubular basement membrane for its targeted sequestration in kidney parenchyma. In this process, the tracer participates in physiologic glomerular filtration and tubular reabsorption while escaping lysosomal catabolism and urinary clearance. Methods: To specifically target renal receptors in navigating the urinary passage in the kidney, we produced a recombinant fusion protein with two separate functional parts: a polybasic PBT segment derived from human Vascular Endothelial Growth Factor and Fc segment of IgG1. The chimeric fusion of PBT-Fc was labeled with radionuclide 99m Tc and tested in rodent models of kidney diseases. Planar scintigraphy and single-photon emission computerized tomography (SPECT) were performed to evaluate renal-specificity of the tracer. Results: When injected in mouse and rat, following a brief 10 - 15 min dynamic redistribution phase in circulation, ~ 95% of the [ 99m Tc]-PBT-Fc signal was concentrated in the kidney and lasted for hours without urinary loss or surrounding tissue activities. Long-lasting tracer signals in the kidney cortex in conjunction with SPECT greatly augmented the image quality in detecting pathological lesions in a variety of disease models, including ischemic acute kidney injury, drug-induced renal toxicity, and chronic kidney disease from renin-angiotensin system (RAS) overactivation. Conclusion: Exclusive renal retention of the recombinant radiotracer greatly facilitated static-phase signal acquisition by SPECT and achieved submillimeter spatial resolution of kidney alternations in glomerular and tubular disease models.

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“…This probe also has attachment points for customizable multi-purpose needle guided systems. Its curved shape allows for deeper penetration into the body, making it suitable for visualizing organs like the liver, or special fusion imaging possibilities of the kidneys [15]. At last one endocavitary probe was applied, the E8CS (GE Healthcare, Waukesha, Wisconsin, USA) for abdominal or even gynaecological applications.…”

Section: Probesmentioning

confidence: 99%

Visualization of Effectiveness: The Use of a Colored Cleaning Wipe Set for Visible Disinfection of Ultrasound Probes

Kühnel,

Gühne

2024

Preprint

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The German health authorities' guidelines for medical devices in 2012 highlighted the importance of cleaning ultrasound probes, emphasizing their validation and reliability. In addition to automated and validated options, alternative manual methods such as wipe disinfection have gained traction due to their independence from additional hardware. The study examines the effectiveness of manual disinfection methods, specifically the cleaning process, addressing concerns raised by the Robert Koch Institute regarding the lack of validation for wipe disinfection of semi-critical devices. The EQUINOS colored wipe disinfection kit identified wetting gaps in all cleanings across four probes tested. The results indicate significant challenges in ensuring complete surface wetting, particularly in complex body parts such as clip-on areas and shapes for additional biopsy attachments, suggesting that manual methods alone may not adequately mitigate the risk of infection transmission (p value .001). The study concludes that while manual disinfection methods are a viable alternative to automated reprocessing, there is a critical need for enhanced training and potentially the development of more effective manual disinfection techniques or colored wipes to ensure patient safety and compliance with healthcare hygiene standards.

show abstract

DMSA-camSPECT/US fusion imaging of children’s kidneys – Proof of feasibility

Cited by 6 publications

References 19 publications

Visualization of Effectiveness: The Use of a Set of Colored Cleaning Wipes for Visible Disinfection of Ultrasound Probes

Visualization of Effectiveness: The Use of a Set of Colored Cleaning Wipes for Visible Disinfection of Ultrasound Probes

Targeted design of a recombinant tracer for SPECT renal imaging

Visualization of Effectiveness: The Use of a Colored Cleaning Wipe Set for Visible Disinfection of Ultrasound Probes

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