A current phenomenon of great concern in the medical community in developing countries is raising multi drug resistant organisms, and their problems with curing the infections in children. Most urinary tract infection (UTIs) in children are monomicrobic, often caused by Escherichia coli (60 to 80 percent of cases). Infected children need more care because they threaten by death and for treatment of them expensive drugs should be used, so in this study we evaluated the multi drug resistant strains of E. coli isolated from urinary tract infection in Iranian girl childrens. Materials and Methods This laboratory search was performed in DEY laboratory in Isfahan .Totally 1027 samples, collected from urine samples of girl children, aged from 1 month to 10 years. After confirmation of urine culture which is expressive of urinary infection, gram stain and biochemical differential test was done for the isolates and then susceptibility of all of them to 3 different antibiotics was determined by standard disk diffusion method. Results From 1027 study sample, frequency of UTIs in girl children's was 5.35%. Seventy nine percent of the isolates were multi drug resistant strains. Conclusion The antibiogram patterns of isolates showed a high percentage of multi drug resistant phenotype among the E. coli strains. With regard to the present data and high percentage of multi drug resistant strains of E. coli, regular monitoring antimicrobial drug resistance in the different areas is necessary to prevent unsuitable utilization of drugs which is the most important cause of emerging multi drug resistant strains.