DMSO containing ruthenium(ii) hydrazone complexes: in vitro evaluation of biomolecular interaction and anticancer activity

Abstract

Synthesis, spectral, electrochemical and single crystal X-ray diffraction data of a new series of DMSO containing bivalent ruthenium hydrazone complexes are presented. XRD data of two of the new complexes revealed an octahedral coordination around the ruthenium ion satisfied by NOS2Cl2 atoms. Electrochemical studies showed the metal centred, quasi-reversible, one-electron redox behaviour of the new complexes. The binding of these complexes with biomolecules such as calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) protein investigated by different spectrophotometric methods revealed an intercalative mode of interaction. The in vitro cytotoxicity of these complexes evaluated by the MTT assay on a panel of cancer and normal cell lines indicated that the above complexes are more toxic to cancer cells with a few micromolar concentrations as the IC50 value, but are significantly less toxic to normal cell lines. The observed variations in the binding interactions and cytotoxicity of the complexes were attributed to the nature of the hydrazide moiety of the hydrazones that influences their biological activities.

Oxovanadium(IV) and ruthenium(II) carbonyl complexes of ONS‐donor ligands derived from dehydroacetic acid and dithiocarbazate: Synthesis, characterization, antioxidant activity, DNA binding and in vitro cytotoxicity

“…The obtained results revealed that the compounds have a strong association with DNA base pairs through intercalation mode . The hypochromic feature is due a decrease in transition energies resulted from the overlapping π* orbitals of the intercalated compounds with π orbitals of DNA base pairs . Based on binding constant values for the compounds, the binding strength follows the order: …”

“…[36,68] The hypochromic feature is due a decrease in transition energies resulted from the overlapping π* orbitals of the intercalated compounds with π orbitals of DNA base pairs. [69,70] Based on binding constant values for the compounds, the binding strength follows the order: Due to the strong intercalating ability of DNA base pairs with EB, intense emission fluorescence at λ max 580 nm is obtained. Figure 9 shows the influence of the addition of various concentrations of the test compounds to pretreated DNA-EB solution.…”

Ru(II)-based complexes with N-(acyl)-N′,N′-(disubstituted)thiourea ligands: Synthesis, characterization, BSA- and DNA-binding studies of new cytotoxic agents against lung and prostate tumour cells

“…The magnitude of the BSA-binding constant of the complexes 1-4, compared with other Ru(II) complexes reported recently [60], suggests a moderate interaction with BSA molecule.…”

Organoruthenium (II) complexes featuring pyrazole‐linked Schiff base ligands: Crystal structure, DNA/BSA interactions, cytotoxicity and molecular docking

“…From Figure , it is observed that at the end of the titration, the decrease in the emission intensity is about 29% and 35.38%. This is observed due to the intercalation between metal complex and DNA base pairs …”