DNA addition polymerization with logic operation for controllable self-assembly of three-dimensional nanovehicles and combinatorial cancer therapy

Yue, Shuzhen; Qiao, Zhenjie; Yu, Kaixin; Hai, Xin; Li, Yuwei; Li, Yuanfang; Song, Weiling; Bi, Sai

doi:10.1016/j.cej.2020.127258

Cited by 19 publications

(16 citation statements)

References 46 publications

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“…A powerful signal amplification method is the key determinant for sensitive imaging of underexpressed miRNA target molecules. Most signal amplification techniques based on rolling circle amplifications and nuclease-assisted amplifications involve the employment of enzymes, making them unsuitable for live-cell imaging. − As alternative amplification strategies, the catalytic hairpin assembly (CHA) that relies on the assembly formation of dsDNAs from two hairpin DNAs by single-stranded nucleic acid initiator sequences and metal ion-dependent DNAzyme (Dz) that catalyzes the cleavage of DNA substrates with the assistance of specific metal ion cofactors have attracted intense attention in bimolecular imaging very recently, due to their independence of enzymes. − For instance, a method based on CHA amplification for the analysis of intracellular miRNA-155 has been proposed by transfecting the free DNA sensing probes into cells via the LipoHigh transfection reagent, and a target-activated Dz for multicolor imaging of miRNA-223 and miRNA-222 has also been developed by delivering the DNA probes into cells through similar cation liposomes . However, these liposome-based probe delivery methods can potentially lead to the use of toxic transfection reagents.…”

Section: Introductionmentioning

confidence: 99%

3D DNA Scaffold-Assisted Dual Intramolecular Amplifications for Multiplexed and Sensitive MicroRNA Imaging in Living Cells

Yang

Gan

et al. 2021

Anal. Chem.

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The simultaneous live-cell imaging of multiple intracellular and disease-related microRNAs (miRNAs) with low abundances is highly important to enhance specificity and accuracy for disease diagnosis. On the basis of the improved cell internalization and accelerated reaction kinetics, we develop a three-dimensional (3D) DNA nanoprobe that integrates intramolecular DNAzyme (intra-Dz) and catalytic hairpin assembly (intra-CHA) amplifications to simultaneously monitor multiple miRNAs in living cells. The sensing components are loaded on a DNA scaffold via the sticky-end hybridization of the DNA sequences to increase the local concentrations of the signal probes. The miRNA-21 and miRNA-155 target sequences can trigger intra-Dz and -CHA amplifications on the nanoprobes to show significantly amplified and distinct fluorescence at different wavelengths for simultaneously monitoring low levels of miRNAs. Real-time fluorescence microscopy reveals that such a 3D DNA nanoprobe design with the intra-Dz and -CHA amplifications can accelerate the reaction rate compared to that of the conventional free Dz and CHA because of the increased local concentrations of the sensing components. Importantly, the 3D DNA nanoprobe has desirable stability and biocompatibility and can be readily delivered into living cells to achieve multiplexed and highly sensitive sensing of intracellular miRNA-155 and miRNA-21 sequences. With the demonstration of its intracellular application, the developed 3D DNA nanoprobe thus holds promising potential for biological studies and accurate disease diagnosis.

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Section: Introductionmentioning

confidence: 99%

3D DNA Scaffold-Assisted Dual Intramolecular Amplifications for Multiplexed and Sensitive MicroRNA Imaging in Living Cells

Yang

Gan

et al. 2021

Anal. Chem.

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“…With the continuous progress of synergistic chemo/GT, the construction of multifunctional DNA nanocarriers to coload combinatorial RNA (co-RNA) and therapeutic drugs have been exploited. Recently, our group fabricated a DNA addition polymerization reaction to self-assemble DNA nanospheres for simultaneously loading two different siR-NAs and therapeutic drugs for combinatorial chemo/GT of anaplastic large cell lymphoma (ALCL) [144]. In brief, the DNA addition polymerization reaction using DNA dimers and hairpins as monomers was catalyzed by DNA initiators based on the catalytic hairpin assembly (CHA), leading to the formation of DNA nanospheres with a large number of repeated structural units.…”

Section: Chemotherapy/gene Therapymentioning

confidence: 99%

DNA Nanotechnology for Multimodal Synergistic Theranostics

Qiao

Song

et al. 2021

J. Anal. Test.

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Over the past decade, DNA nanotechnology has developed rapidly due to its unique characteristics, such as excellent biocompatibility, high programmability, good predictability, automatically chemical synthesis, and so on. So far, a variety of DNA-based nanostructures, from small to large and simple to complex, have been designed and synthesized with controllable size and shape in one, two, or three dimensions. Therefore, DNA has become a kind of competitive materials for biosensing, bioimaging and biomedicine. In particular, the integration of DNA nanotechnology with multimodal synergistic theranostics can not only achieve accurate cancer diagnosis by the sensitive and accurate detection of cancer biomarkers, but also achieve enhanced anti-cancer therapeutic efficacy, which promote the development of DNA nanotechnology and nanomedicine. In this review, we first give a comprehensive introduction of DNA nanotechnology, and then summarize the DNA self-assembly and amplification strategies for the construction of functional nanoplatforms for multimodal synergistic theranostics. Finally, the challenges and opportunities faced by DNA nanotechnology in biomedicine are discussed.

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“…Unfortunately, these nanomaterials are still challenged by lack of recognition ability for targets, poor stability, sophisticated preparation and functionalization processes. 30,31 In contrast, the three-dimensional DNA nanostructures, including DNA polyhedrons, 32 DNA dendrimers, 33 DNA nanoflowers, 34 and DNA nanospheres, 35 constructed through the simple DNA self-assembly have been used as nanocarriers and widely applied in the field of biosensing, biomedicine, and drug delivery. Moreover, the unique Watson−Crick basepairing endows these nanocarriers with excellent specificity and robust stability in complex body liquid.…”

Section: ■ Introductionmentioning

confidence: 99%

An Ultrasensitive Electrochemical Biosensor Integrated by Nicking Endonuclease-Assisted Primer Exchange Reaction Cascade Amplification and DNA Nanosphere-Mediated Electrochemical Signal-Enhanced System for MicroRNA Detection

Chen

Dang

et al. 2022

Anal. Chem.

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Specific and sensitive microRNAs (miRNAs) detection is essential to early cancer diagnosis. The development of these technologies including functional nuclease-mediated target amplification and DNA nanotechnology possesses tremendous potential for the high-performance detection of miRNAs in the accurate diagnosis of disease. In this study, we have established an ultrasensitive electrochemical biosensor by combining nicking endonuclease-assisted primer exchange reaction (PER) cascade amplification with a DNA nanosphere (DNS)-mediated electrochemical signal-enhanced system for the detection of miRNA-21 (miR-21). The cascade amplification is initiated by a nicking endonuclease that can cleave specific DNA substrates and highly amplify translation of the target to single-stranded DNA fragments (sDNA). Then, the PER cascade is powered by strand-displacing polymerase and generates a large amount of nascent single-stranded connector DNA (cDNA) via sDNA triggering of the dumbbell probe (DP), thus achieving the cascade amplification of the target. Finally, the DNS loaded with plenty of electroactive substances can be captured on the electrode via cDNA for further enhancing the electrochemical signal and highly sensitive detection of miR-21. The proposed electrochemical biosensor exhibits a wide detection range of 1 aM to 0.1 nM and a low detection limit of 0.58 aM. The excellent selectivity allows the biosensor to discriminate miR-21 from other miRNAs, even the one base-mismatched sequence. Moreover, the practicability of the biosensor is investigated by analyzing miR-21 in human serum and cancer cell lysate. Therefore, our proposed nicking endonuclease-assisted PER cascade amplification strategy provides a powerful platform for the early detection of miRNA-related disease and molecular diagnosis.

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DNA addition polymerization with logic operation for controllable self-assembly of three-dimensional nanovehicles and combinatorial cancer therapy

Cited by 19 publications

References 46 publications

3D DNA Scaffold-Assisted Dual Intramolecular Amplifications for Multiplexed and Sensitive MicroRNA Imaging in Living Cells

3D DNA Scaffold-Assisted Dual Intramolecular Amplifications for Multiplexed and Sensitive MicroRNA Imaging in Living Cells

DNA Nanotechnology for Multimodal Synergistic Theranostics

An Ultrasensitive Electrochemical Biosensor Integrated by Nicking Endonuclease-Assisted Primer Exchange Reaction Cascade Amplification and DNA Nanosphere-Mediated Electrochemical Signal-Enhanced System for MicroRNA Detection

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