DNA Adducts as Biomarkers for Oxidative and Genotoxic Stress from Pesticides in Crop Plants

Boerth, Donald W.; Eder, Erwin; Stanks, John R.; Wanek, Paul; Wacker, M.; Gaulitz, Scott; Skypeck, Daniel; Pandolfo, Derek; Yashin, Michael

doi:10.1021/jf072816q

Cited by 12 publications

(5 citation statements)

References 80 publications

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“…A study on radio chromatograms using 32P post labeling in the plants such as grapes, bush beans, soybeans, pumpkins, and cucumbers treated with pesticides has shown high level of DNA adducts despite of untreated plants. The study also indicates that treated plants have undergone severe oxidative stress and lipid per oxidation [48]. Thus, DNA adducts could be used as biomarker in differentiation of organic foods from nonorganic foods.…”

Section: Organic Foodsmentioning

confidence: 77%

DNA as a Biomaterial in Diagnosis of Food Adulteration and Food Safety Assurance

Muthu¹

2017

RDMS

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Apart from general applications of DNA, it can also be used as tool to assure the food safety, which is the main aspect of this review. Increasing GMO productions also increased the concern regarding the adulteration of GMO food products in our daily life. Countries around the world, raising the regulations regarding the GMO products to the consumer's table. Subsequently, the mode of adulteration in food products with GMO products feels difficult to identify. The food products also adulterer with low cost products which can also be detected by the isolation and identification of DNA from that product. The main focus on this review is to discuss the utilization of DNA as an effective tool to detect the adulteration and GMO content in various food products like oil, honey, beverages, baked products, animal feed, fried products, adulteration of milk and milk products. In the field of food safety, microbial contamination is also a major problem. Isolation of DNA from food can also reveals the presence of microbial contamination in the earlier stage. Also, the review emphases the way of utilizing DNA in differentiating organic foods from non-organic foods. ISSN: 2576-8840How to cite this article: Duraimurugan K, Narendhran S, Manikandan M. DNA as a Biomaterial A food should be free from microbial contamination until it reaches the consumers table. All kind of safety measures must be ensured by industries to make it possible. But still microbes are found in food products by any source, leading to the spoilage of food product and causing food borne infections to the consumers. Home cooked food samples collected from the villages in Malawi has shown to be contaminated upto 35% of pathogenic microbes and by further investigation, it was found that contamination of food samples by poor handling and unhygienic conditions [11]. Microbial contamination is a crucial problem in various food industries causing major economic losses. Various industries like dairy, poultry industries follow various cutting-edge technologies to reduce the microbial contamination in end product [12,13]. Microbial contamination can be explored by various physical methods including microscopic and culture depend approaches. But these techniques have various limitations in the confirmation of microbial presence. In present decades, PCR based methods are highly adopted due to its high accuracy and reliability [14].In the present review, we discuss the utilization of DNA as an effective tool in ensuring the quality and safety in various foods and processed food products (Figure 1). DiscussionDifferent DNA isolation methods from food products [20]. Apart from the standard protocols and commercial kits, various modified protocols are also adopted by researchers in order to enhance the efficiency of quality and quantity of extracted DNA [21]. DNA based detection of adulteration and GMO in food productsMilk and milk products: Milk stands as a source of high protein based food for vegetarians around the world. Commercial milk is available in the f...

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Section: Organic Foodsmentioning

confidence: 77%

DNA as a Biomaterial in Diagnosis of Food Adulteration and Food Safety Assurance

Muthu¹

2017

RDMS

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“…Pesticides induced adduct formation due to the binding with DNA. Reactions of DNA bases with pesticides or their metabolites alter its structure and prevent DNA replication [ 55 ]. So, we can assume that the mechanism of pesticide toxicity in vitro is not associated with ROS production, and it is most likely that pesticides directly interact with mtDNA and damage it.…”

Section: Discussionmentioning

confidence: 99%

High Doses of Pesticides Induce mtDNA Damage in Intact Mitochondria of Potato In Vitro and Do Not Impact on mtDNA Integrity of Mitochondria of Shoots and Tubers under In Vivo Exposure

Alimova

Sitnikov

Pogorelov

et al. 2022

IJMS

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It is well known that pesticides are toxic for mitochondria of animals. The effect of pesticides on plant mitochondria has not been widely studied. The goal of this research is to study the impact of metribuzin and imidacloprid on the amount of damage in the mtDNA of potato (Solanum tuberosum L.) in various conditions. We developed a set of primers to estimate mtDNA damage for the fragments in three chromosomes of potato mitogenome. We showed that both metribuzin and imidacloprid considerably damage mtDNA in vitro. Imidacloprid reduces the rate of seed germination, but does not impact the rate of the growth and number of mtDNA damage in the potato shoots. Field experiments show that pesticide exposure does not induce change in aconitate hydratase activity, and can cause a decrease in the rate of H2O2 production. We can assume that the mechanism of pesticide-induced mtDNA damage in vitro is not associated with H2O2 production, and pesticides as electrophilic substances directly interact with mtDNA. The effect of pesticides on the integrity of mtDNA in green parts of plants and in crop tubers is insignificant. In general, plant mtDNA is resistant to pesticide exposure in vivo, probably due to the presence of non-coupled respiratory systems in plant mitochondria.

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“…Other compounds from the formulation, such as sugars, are introduced into commercial products as phagostimulants for more effective ingestion by larvae. Many products can elicit plant reactions and/or alter cuticular permeability . Signalling pathways elicited by products may be common with those elicited by sugars; the worst case would be elicitation of antagonist signalling pathways to those elicited by the sugars.…”

Section: Discussionmentioning

confidence: 99%

Foliar application of microdoses of sucrose to reduce codling moth Cydia pomonella L. (Lepidoptera: Tortricidae) damage to apple trees

Arnault

Lombarkia

Joy-Ondet

et al. 2016

Pest Management Science

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DNA Adducts as Biomarkers for Oxidative and Genotoxic Stress from Pesticides in Crop Plants

Cited by 12 publications

References 80 publications

DNA as a Biomaterial in Diagnosis of Food Adulteration and Food Safety Assurance

DNA as a Biomaterial in Diagnosis of Food Adulteration and Food Safety Assurance

High Doses of Pesticides Induce mtDNA Damage in Intact Mitochondria of Potato In Vitro and Do Not Impact on mtDNA Integrity of Mitochondria of Shoots and Tubers under In Vivo Exposure

Foliar application of microdoses of sucrose to reduce codling moth Cydia pomonella L. (Lepidoptera: Tortricidae) damage to apple trees

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