DNA adducts in urothelial cells: Relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke

Víneis, Paolo; Talaska, Glenn; Malaveille, Christian; Bartsch, Helmut; Martone, Tiziana; Sithisarankul, Pornchai; Strickland, Paul T.

doi:10.1002/(sici)1097-0215(19960126)65:3<314::aid-ijc6>3.3.co;2-1

Cited by 18 publications

(17 citation statements)

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“…In people eating charbroiled beef, the urinary 1-HOP glucuronide concentration during and immediately after the feeding period was significantly correlated with the mean PAH-DNA adduct level during the same period (36). In smokers, a statistically nonsignificant association between 1-HOP glucuronide and a specific adduct in urothelial cells was observed (37).…”

Section: Discussionmentioning

confidence: 97%

Biomarkers for exposure to ambient air pollution--comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress.

Autrup

Daneshvar

Dragsted

et al. 1999

Environ Health Perspect

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Human exposure to genotoxic compounds present in ambient air has been sdied using selected biomarkers in nonsmoking Danish bus drivers and pol workers. A li n variadion in biomarker levels was observed. Significantly higher levels of bulky carcinogen-DNA adducts (75.42 adducts/108 nucleotides) and of 2-amino-apidic semialdehyde (AAS) in plasma proteins (56.7 pmollmg protein) were observed in bus drivers working in the central part of Copenhagen, Denmark In contrast, significantly higher levels of AAS in hemoglobin (55,8 pmollmg protn), malondialdeh in plasma (0.96 nol/m plasma) nd polycic aromatic hydrocarbon (PAH)-albumin adduct (3.38 finol/pg lbumin) were observed in the suburban group. The biomarker levels in postal workers were similar to the levels in suburban bus drivers. In the combined group of bus drivers and postal workers, negative corlations were observed between bulky carcinogen-DNA adduct and PAH-albumin levels (p -0.005), and between DNA adduct and .y-glutamyl semialdhyde (GGS)

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Section: Discussionmentioning

confidence: 97%

Biomarkers for exposure to ambient air pollution--comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress.

Autrup

Daneshvar

Dragsted

et al. 1999

Environ Health Perspect

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“…Second, the concentrations used in this study (1-50 M for 4-ABP and 1 M for B[a]P, are assumedly much higher than in the bladder in vivo, although these concentrations have not been analysed up to now. In such studies DNA adducts of, e.g., 4-ABP were determined in bladder tissue (Kadlubar and Badawi 1995;Vineis et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

et al. 2008

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Aromatic amines have been shown to cause bladder cancer. However, epithelial cells of the urinary bladder, cells of origin of bladder cancer, may be exposed to numerous substances besides aromatic amines. In the present study, we analysed possible interactions between the aromatic amines 4-aminobiphenyl (4-ABP) as well as 2-naphthylamine (2-NA) and the polycyclic aromatic hydrocarbon benzo[a]pyrene (B[a]P). For this purpose we incubated primary porcine urinary bladder epithelial cells (PUBEC) with concentrations of 1 to 50 M 4-ABP with and without coexposure to B[a]P. As expected B[a]P increased mRNA expression of cytochrome P450 1A1 (CYP1A1), whereas 4-ABP had no eVect. However, when co-exposed 4-ABP enhanced the induction of CYP1A1 by B[a]P. This result was conWrmed by Western blot analysis of CYP1A1 protein expression. A similar eVect as for CYP1A1 was also observed for cyclooxygenase-2 (COX-2) and UDP-glucuronosyltransferase 1 (UGT1). Next, we studied co-exposures of 2-NA and B[a]P. Similar as for 4-ABP also 2-NA enhanced B[a]P-mediated induction of CYP1A1. Our results demonstrate that some aromatic amines may enhance the inXuence of B[a]P on Ah receptor-dependent genes.

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“…Moreover, the analysis of DNA adducts in urothelial cells of smokers indicates that DNA damage may be due to aromatic amines rather than to other tobacco carcinogens, such as benzo(a)pyrene (Vineis et al, 1996).…”

mentioning

confidence: 99%

Analysis of p53, p16MTS1, p21WAF1 and H-ras in archived bladder tumours from workers exposed to aromatic amines

Sørlie

Martel‐Planche²,

Hainaut³

et al. 1998

Br J Cancer

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Summary Exposure to aromatic amines is considered a major risk factor for the development of bladder cancer. In this study, we have analysed the pattern of point mutations in several tumour genes in 21 cases of bladder cancer arising among western European workers exposed to aromatic amines in an attempt to determine whether this exposure may be associated with a unique spectrum of mutations. Of the four genes analysed (p53, p16mTs1, p21WAF1 and H-ras), only p53 showed a high frequency of mutations (in 8 out of 21 cases, 38%). Two mutations were found in p16, one in H-ras and none in p21 exon 3. All mutations were at G:C base pairs, mostly at non-CpG residues. This spectrum of mutations, which is highly suggestive of an involvement of exogenous carcinogens, is however identical to the spectrum of p53 mutations detected in bladder cancers of the general population. In exposed workers, p53 mutations were associated with tumour grade and with high occupational and tobacco exposure. Taken together, our data suggest that the same carcinogens may be responsible for the development of bladder cancers in workers exposed to aromatic amines and in the general population.

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DNA adducts in urothelial cells: Relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke

Cited by 18 publications

References 0 publications

Biomarkers for exposure to ambient air pollution--comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress.

Biomarkers for exposure to ambient air pollution--comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress.

Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

Analysis of p53, p16MTS1, p21WAF1 and H-ras in archived bladder tumours from workers exposed to aromatic amines

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