DNA and lipid molecular stratigraphic records of haptophyte succession in the Black Sea during the Holocene

Coolen, Marco J. L.; Sáenz, James; Giosan, Liviu; Trowbridge, N.; Dimitrov, Petko; Dimitrov, Dimitar; Eglinton, T. I.

doi:10.1016/j.epsl.2009.05.029

Cited by 81 publications

(108 citation statements)

References 73 publications

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“…Earlier eDNA studies on marine sediments assumed that DNA preservation is proportional to the preservation of organic matter and, thus, prioritized sampling in organic-rich sediment layers (Coolen et al, 2009). Yet, recent experimental research and field studies suggest that the primary structure of DNA molecules is adsorbed to solid particles and molecules preserved in this way may form an archive of extracellular DNA regardless of the organic content of the sediment (Corinaldesi et al, 2007(Corinaldesi et al, , 2011(Corinaldesi et al, , 2014Torti et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology

et al. 2017

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Abstract. Deep-sea sediments constitute a unique archive of ocean change, fueled by a permanent rain of mineral and organic remains from the surface ocean. Until now, paleoecological analyses of this archive have been mostly based on information from taxa leaving fossils. In theory, environmental DNA (eDNA) in the sediment has the potential to provide information on non-fossilized taxa, allowing more comprehensive interpretations of the fossil record. Yet, the process controlling the transport and deposition of eDNA onto the sediment and the extent to which it preserves the features of past oceanic biota remains unknown. Planktonic foraminifera are the ideal taxa to allow an assessment of the eDNA signal modification during deposition because their fossils are well preserved in the sediment and their morphological taxonomy is documented by DNA barcodes. Specifically, we re-analyze foraminiferal-specific metabarcodes from 31 deep-sea sediment samples, which were shown to contain a small fraction of sequences from planktonic foraminifera. We confirm that the largest portion of the metabarcode originates from benthic bottom-dwelling foraminifera, representing the in situ community, but a small portion (< 10 %) of the metabarcodes can be unambiguously assigned to planktonic taxa. These organisms live exclusively in the surface ocean and the recovered barcodes thus represent an allochthonous component deposited with the rain of organic remains from the surface ocean. We take advantage of the planktonic foraminifera portion of the metabarcodes to establish to what extent the structure of the surface ocean biota is preserved in sedimentary eDNA. We show that planktonic foraminifera DNA is preserved in a range of marine sediment types, the composition of the recovered eDNA metabarcode is replicable and that both the similarity structure and the diversity pattern are preserved. Our results suggest that sedimentary eDNA could preserve the ecological structure of the entire pelagic community, including nonfossilized taxa, thus opening new avenues for paleoceanographic and paleoecological studies.

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Section: Discussionmentioning

confidence: 99%

Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology

et al. 2017

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“…This suggests that the two types of alkenones derive from different organisms, which -although living in the same environment -fractionate differently. Although Coolen et al (2009) suggest that E. huxleyi in the Black Sea is the most likely source for the C 36 alkenones, it is difficult to reconcile the differences in isotopic fractionation with this explanation. Overall, the unusual double bond positions, in combination with the significantly enriched δD values for the alkenones, suggest that the C 36 alkenones do not derive from any known alkenone-producing haptophytes, but are instead produced by an unknown species.…”

Section: Discussionmentioning

confidence: 89%

“…In Unit I Black Sea sediments, only haptophyte DNA belonging to E. huxleyi was detected (Coolen et al, 2006), whereas in Unit II sediments, DNA from both E. huxleyi and Isochrysis spp. was detected (Coolen et al, 2009). Isochrysis spp.…”

Section: Introductionmentioning

confidence: 99%

“…DNA was detected, whereas C 36 alkenones were abundant in the sediments. Coolen et al (2009) proposed three strains of E. huxleyi as a possible source of the C 36:2 EK, since the presence of their fossil DNA coincided with, and was unique for, a period during which the abundance of C 36:2 EK in the Black Sea sediments was greatest.…”

Section: Introductionmentioning

confidence: 99%

“…C 35 and C 36 alkenones with the same double bond positions as the Black Sea alkenones, were tentatively assigned in a cultured strain of G. oceanica (Rontani et al, 2006b), but the distribution differed markedly from those in Black Sea Unit II sediments. In an effort to identify the haptophytes responsible for alkenone production in the Black Sea sediments, Coolen et al (2006Coolen et al ( , 2009 used fossil DNA to reconstruct haptophyte assemblages occurring in the water column during the Holocene. In Unit I Black Sea sediments, only haptophyte DNA belonging to E. huxleyi was detected (Coolen et al, 2006), whereas in Unit II sediments, DNA from both E. huxleyi and Isochrysis spp.…”

Section: Introductionmentioning

confidence: 99%

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Unusual C35 to C38 alkenones in mid-Holocene sediments from a restricted estuary (Charlotte Harbor, Florida)

Soelen

Lammers

Eglinton

et al. 2014

Organic Geochemistry

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Unusual C 35 to C 38 alkenones were identified in mid-Holocene (8-3.5 kyr BP) sediments from a restricted estuary in southwest Florida (Charlotte Harbor). The distribution was dominated by a C 36 diunsaturated (ω15,20) ethyl ketone, identical to the one present in Black Sea Unit 2 sediments. Other unusual alkenones were tentatively assigned as a C 35:2 (ω 15,20) methyl ketone, a C 37:2 (ω 17,22) methyl ketone and a C 38:2 (ω 17,22) ethyl ketone. In late Holocene sediments < 3.5 kyr BP, the common C 37 to C 39 alkenones were found. Compound-specific 14 C, 13 C, and D isotope measurements were used to constrain the possible origin of the alkenones. Conventional radiocarbon ages of alkenones and higher plant-derived long chain n-alcohols indicated no significant difference in age between mid-Holocene alkenones and higher plant n-alcohols. Both alcohols and alkenones were offset vs. calibrated ages of shell fragments in the same sediment core, which suggests they were pre-aged by 500-800 yr, implying resuspension and redistribution of the fine-grained sedimentary particles with which they are associated. The hydrogen isotopic (δD) composition (-190‰ to -200‰) of the C 37 and C 38 alkenones in the late Holocene sediments is in line with values for coastal haptophytes in brackish water. However, the unusual C 36 and C 38 alkenones from the mid Holocene sediments were enriched in D (by ca. 100‰) vs. the late Holocene alkenones. Also, δ 13 C values of mid-Holocene alkenones were consistently offset compared with late Holocene alkenones (-21‰ to -22‰ and -22‰ to -23‰, respectively). We suggest that the alkenones in Charlotte Harbor were produced by unknown alkenone-producing haptophyte.

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Successional blooms of alkenone‐producing haptophytes in Lake George, North Dakota: Implications for continental paleoclimate reconstructions

Theroux

Huang

Toney

et al. 2019

Limnology & Oceanography

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Alkenone‐derived paleotemperature reconstruction holds great promise in lake environments. However, the occurrence of multiple species of alkenone‐producing haptophyte algae in a single lake can complicate the translation of alkenone unsaturation to temperature if each species requires an individual temperature calibration. Here, we present the first systematic monitoring of two alkenone‐producing haptophytes throughout the course of a seasonal cycle in Lake George, North Dakota, using a combined approach of DNA sequencing and alkenone lipid characterization. Field sampling revealed a nonoverlapping haptophyte succession, with both an early and late season haptophyte bloom event. Culturing experiments demonstrated that the two haptophyte species responsible for these blooms had statistically similar alkenone‐temperature responses, although the culture‐based calibrations were distinct from the in situ calibration. Bloom timing of each haptophyte species corresponded to surface‐water temperatures that differed by more than 10°C, revealing that changes in bloom intensities for each species will skew the sediment‐inferred temperatures to a different stage of the growth season. These results highlight the importance of accounting for bloom timing when interpreting alkenone‐derived temperatures in sediment cores, especially in lakes that experience large seasonal fluctuations in water column temperature and salinity.

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DNA and lipid molecular stratigraphic records of haptophyte succession in the Black Sea during the Holocene

Cited by 81 publications

References 73 publications

Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology

Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology

Unusual C35 to C38 alkenones in mid-Holocene sediments from a restricted estuary (Charlotte Harbor, Florida)

Successional blooms of alkenone‐producing haptophytes in Lake George, North Dakota: Implications for continental paleoclimate reconstructions

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