DNA and morphological markers for a Russian wheat aphid resistance gene

Arzani, Ahmad; Peng, Junhua; Lapitan, Nora L. V.

doi:10.1007/s10681-004-2898-4

Cited by 16 publications

(7 citation statements)

References 25 publications

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“…The allele size (175 bp) obtained from PI372129 correspond to that obtained by Liu et al [36]. The same 175 bp allele was observed in all the Halt plants tested, however, it conflicted with a 225 bp allele size that was reported by Arzani et al [35] for Xgwm337. The 225 bp allele size was observed for Ankor, Ripper, 18 FAWWON-SA 57, some plants of Corwa and PI586956.…”

Section: Discussionsupporting

confidence: 82%

“…Currently, none of the RWA resistance genes have been cloned or fully characterised, hence the location of these genes was inferred through markers in genetic mapping studies. Genetic components involved in RWA resistance have also been reported on chromosomes 1A, 1B, 1D, 3A, 3B, 4A, 4D, 5B, 5D, 6A, 6D, 7A, 7B and 7D [7,9,16,18,19,[21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide association analysis of Russian wheat aphid (Diuraphis noxia) resistance in Dn4 derived wheat lines evaluated in South Africa

Kisten

Tolmay²,

Mathew

et al. 2020

PLoS ONE

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Russian wheat aphid (RWA; Diuraphis noxia Kurdjumov) resistance on the 1D chromosome of wheat has been the subject of intensive research. Conversely, the deployment of the Dn4 derived RWA resistant varieties diminished in recent years due to the overcoming of the resistance it imparts in the United States of America. However, this resistance has not been deployed in South Africa despite reports that Dn4 containing genotypes exhibited varying levels of resistance against the South African RWA biotypes. It is possible that there may be certain genetic differences within breeding lines or cultivars that influence the expression of resistance. The aim of this study was to identify single nucleotide polymorphism (SNP) markers associated with resistance to South African RWA biotypes. A panel of thirty-two wheat lines were phenotyped for RWA resistance using four South African RWA biotypes and a total of 181 samples were genotyped using the Illumina 9K SNP wheat chip. A genome wide association study using 7598 polymorphic SNPs showed that the population was clustered into two distinct subpopulations. Twenty-seven marker trait associations (MTA) were identified with an average linkage disequilibrium of 0.38 at 10 Mbp. Four of these markers were highly significant and three correlated with previously reported quantitative trait loci linked to RWA resistance in wheat. Twenty putative genes were annotated using the IWGSC RefSeq, three of which are linked to plant defence responses. This study identified novel chromosomal regions that contribute to RWA resistance and contributes to unravelling the complex genetics that control RWA resistance in wheat.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Genome-wide association analysis of Russian wheat aphid (Diuraphis noxia) resistance in Dn4 derived wheat lines evaluated in South Africa

Kisten

Tolmay²,

Mathew

et al. 2020

PLoS ONE

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“…This strategy was based on fi ndings that all non-rye-derived RWA resistance genes characterized thus far map to the D genome (Liu et al, 2001(Liu et al, , 2002(Liu et al, , 2005Ma et al, 1998;Miller et al, 2001). Those SSRs that had been previously linked to RWA resistance genes were included (Arzani et al, 2004; N.L.V. Lapitan, unpublished data, 2010;Liu et al, 2001Liu et al, , 2002Liu et al, , 2005Miller et al, 2001;Simkova et al, 2011).…”

Section: Dna Extraction and Marker Analysismentioning

confidence: 99%

“…Lapitan, unpublished data, 2010;Heyns et al, 2006;Liu et al, 2001Liu et al, , 2002Liu et al, , 2005Miller et al, 2001;Simkova et al, 2011). Both Dn4 and Dn9 were mapped to chromosome 1D, with the former on the short arm and the latter on the long arm (Arzani et al, 2004;Liu et al, 2001Liu et al, , 2002Ma et al, 1998). Dn7 is located on the 1BL.1RS wheat-rye (Secale cereale L.) translocation (Anderson et al, 2003;Lapitan et al, 2007;Marais et al, 1994).…”

mentioning

confidence: 99%

Inheritance and Genetic Mapping of Russian Wheat Aphid Resistance in Iranian Wheat Landrace Accession PI 626580

et al. 2012

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Russian wheat aphid (RWA), Diuraphis noxia (Kurdjumov), is a significant insect pest of wheat {Triticum aestivum L.) and has had a major economic impact worldwide, especially on winter wheat in the western United States. Development of resistant cultivars remains the most viable method for RWA control, although the continuing emergence of new RWA biotypes virulent to deployed resistance genes reinforces the need for the discovery of new sources of resistance. Iranian wheat landrace accession PI 626580 has shown high levels of resistance to RWA biotypes 1 (RWA1) and 2 (RWA2), but the inheritance and chromosomal location of this resistance is unknown. The objectives of this study were to determine the inheritance of RWA2 resistance, identify closely linked markers, and map the chromosomal location of RWA2 resistance found in PI 626580. Bulked segregant analysis was conducted with a mapping population of 154 F^ individuals derived from a single plant selection made from PI 626580 and 'Yuma' (a susceptible wheat cultivar). Phenotypic segregation of RWA2 resistance suggested a single dominant gene, provisionally designated Dn626580. Linkage mapping analysis identified three simple sequence repeat (SSR) markers, Xbarc214, Xgwm473, and Xgwm437, proximally linked to Dn626580 near the centromere on the short arm of chromosome 7D at distances of 1.8, 5.0, and 8.2 cM, respectively. Dn626580, a new resistance gene found in PI 626580, could be used alone or by pyramiding with other resistance genes to develop cultivars with effective RWA resistance.

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“…In initial screening, 210 SSR markers were evaluated, including barc (Song et al 2002), cfa (Sourdille et al 2004), cfd (Guyomarc'h et al 2002, gdm (Pestsova et al 2000), gwm (R€ oder et al 1998) and wmc (Gupta et al 2002) according to the 2004 Chinese Spring wheat consensus map (Somers et al 2004). SSR markers previously linked to RWA resistance genes (Liu et al 2001, Anderson et al 2003, Arzani et al 2004, Lapitan et al 2007a were present among these sets. The sequence information of the primers was obtained from GrainGenes (2012).…”

Section: Methodsmentioning

confidence: 99%

Genetic mapping and inheritance of Russian wheat aphid resistance gene in accession IG 100695

et al. 2016

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The Russian wheat aphid (RWA), Diuraphis noxia (Kurdjumov), is an important pest of small‐grain cereals, particularly wheat, worldwide. The most efficient strategy against the RWA is to identify sources of resistance and to introduce them into susceptible wheat genotypes. This study was conducted to determine the mode of inheritance of the RWA resistance found in ICARDA accession IG 100695, to identify wheat microsatellite markers closely linked to the gene and to map the chromosomal location of the gene. Simple sequence repeat (SSR) marker scores were identified in a mapping population of 190 F2 individuals and compared, while phenotypic screening for resistance was performed in F2 : 3 families derived from a cross between ‘Basribey’ (susceptible) and IG 100695 (resistant). Phenotypic segregation of leaf chlorosis and rolling displayed the effect of a single dominant gene, temporarily denoted Dn100695, in IG 100695. Dn100695 was mapped on the short arm of chromosome 7D with four linked SSR markers, Xgwm44, Xcfd14, Xcfd46 and Xbarc126. Dn100695 and linked SSR markers may be useful for improving resistance for RWA in wheat breeding.

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DNA and morphological markers for a Russian wheat aphid resistance gene

Cited by 16 publications

References 25 publications

Genome-wide association analysis of Russian wheat aphid (Diuraphis noxia) resistance in Dn4 derived wheat lines evaluated in South Africa

Genome-wide association analysis of Russian wheat aphid (Diuraphis noxia) resistance in Dn4 derived wheat lines evaluated in South Africa

Inheritance and Genetic Mapping of Russian Wheat Aphid Resistance in Iranian Wheat Landrace Accession PI 626580

Genetic mapping and inheritance of Russian wheat aphid resistance gene in accession IG 100695

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