DNA aptamers bind specifically and selectively to (1→3)-β-d-glucans

Low, Swee Yang; Hill, Jane E.; Peccia, Jordan

doi:10.1016/j.bbrc.2008.11.087

Cited by 29 publications

(14 citation statements)

References 19 publications

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“…The isolation of aptamers, with the capacity to recognise virtually any class of target molecule with high affinity and specificity, has been made possible by the development of the systematic evolution of ligands by exponential enrichment (SELEX) process [22] , [23] , [24] . Since aptamers can be produced using chemical synthesis or by PCR, the cost of producing aptamers is 10–50 times less than those for producing antibodies [25] . Aptamers have been raised against a wide variety of targets, from small human molecules and viral proteins to whole microorganisms [26] .…”

Section: Introductionmentioning

confidence: 99%

“…Examples include aptamers that detect thrombin, adenosine and cocaine [27] , [28] , [29] , [30] , [31] . Aptamers have also been raised against (1–3)-b-D-glucans, which were the first aptamers developed for the detection of a biotoxin in environmental respiratory diseases [25] . TB-specific aptamers include ssDNA aptamers against Mtb polyphosphate kinase 2 (PPK2) [32] , as well as aptamers against the whole Mtb bacterium [33] .…”

Section: Introductionmentioning

confidence: 99%

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Selection and Application of ssDNA Aptamers to Detect Active TB from Sputum Samples

et al. 2012

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BackgroundDespite the enormous global burden of tuberculosis (TB), conventional approaches to diagnosis continue to rely on tests that have major drawbacks. The improvement of TB diagnostics relies, not only on good biomarkers, but also upon accurate detection methodologies. The 10-kDa culture filtrate protein (CFP-10) and the 6-kDa early secreted antigen target (ESAT-6) are potent T-cell antigens that are recognised by over 70% of TB patients. Aptamers, a novel sensitive and specific class of detection molecules, has hitherto, not been raised to these relatively TB-specific antigens.MethodsDNA aptamers that bind to the CFP-10.ESAT-6 heterodimer were isolated. To assess their affinity and specificity to the heterodimer, aptamers were screened using an enzyme-linked oligonucleotide assay (ELONA). One suitable aptamer was evaluated by ELONA using sputum samples obtained from 20 TB patients and 48 control patients (those with latent TB infection, symptomatic non TB patients, and healthy laboratory volunteers). Culture positivity for Mycobacterium tuberculosis (Mtb) served as the reference standard. Accuracy and cut-points were evaluated using ROC curve analysis.ResultsTwenty-four out of the 66 aptamers that were isolated bound significantly (p<0.05) to the CFP-10.ESAT-6 heterodimer and six were further evaluated. Their dissociation constant (KD) values were in the nanomolar range. One aptamer, designated CSIR 2.11, was evaluated using sputum samples. CSIR 2.11 had sensitivity and specificity of 100% and 68.75% using Youden’s index and 35% and 95%, respectively, using a rule-in cut-point.ConclusionThis preliminary proof-of-concept study suggests that a diagnosis of active TB using anti-CFP-10.ESAT-6 aptamers applied to human sputum samples is feasible.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selection and Application of ssDNA Aptamers to Detect Active TB from Sputum Samples

et al. 2012

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“…For cellobiose targeting aptamers were found K d values ranging from 10 −7 to 10 −5 M (Yang et al, [14]) while for an anti-sialyllactose aptamer the K d was 4.9 μM (Masud et al, [15]). Aptamers selected against beta-1,3-glucans showed dissociation constants around 0.3 μM (Low et al, [16]). …”

Section: Resultsmentioning

confidence: 98%

Selection of Peptidoglycan-Specific Aptamers for Bacterial Cells Identification

Ferreira

Lacerda

Faria

et al. 2014

Appl Biochem Biotechnol

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Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides. Peptidoglycan has a particular composition which makes it a possible target for specific bacterial recognition. Aptamers are single-stranded DNA or RNA oligonucleotides that bind to target molecules with high affinity and specificity. Aptamers can be labeled with different radioisotopes and possess several properties that make them suitable for molecular imaging. The purpose of this study was to obtain aptamers for use as radiopharmaceutical in bacterial infection diagnosis. Two aptamers (Antibac1 and Antibac2) against peptidoglycan were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology. The dissociation constant (K d ) for Antibac1 was 0.415+0.047 μM and for Antibac2 was 1.261+0.280 μM. These aptamers labeled with 32 P showed high affinity for Staphylococcus aureus cells. The binding to S. aureus and Escherichia coli in vitro were significantly higher than for Candida albicans and human fibroblasts, demonstrating their specificity for bacterial cells. These results point Antibac1 and Antibac2 as promising tools for bacterial infections identification.

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“…5. So far, several aptamers have been reported using various types of carbohydrate recognition, including monosaccharides (D-galactose, D-glucose, and D-mannose), 64 disaccharides (cellobiose), 65 oligosaccharides (sialylactose, sialyl Lewis X (sLe X Þ), 66,67 polysaccharides (cellulose, chitin, and curdlan), 68,69 and carbohydrates from glycoproteins (¯brinogen). 70 The detailed and comprehensive introduction can refer to the related review.…”

Section: Small Biological Moleculesmentioning

confidence: 99%

Super-resolution imaging in glycoscience: New developments and challenges

Chen

Tong

Wang

2016

J. Innov. Opt. Health Sci.

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Carbohydrates on cell surfaces play a crucial role in a wide variety of biological processes, including cell adhesion, recognition and signaling, viral and bacterial infection, in°ammation and metastasis. However, owing to the large diversity and complexity of carbohydrate structure and nongenetically synthesis, glycoscience is the least understood¯eld compared with genomics and proteomics. Although the structures and functions of carbohydrates have been investigated by various conventional analysis methods, the distribution and role of carbohydrates in cell membranes remain elusive. This review focuses on the developments and challenges of super-resolution imaging in glycoscience through introduction of imaging principle and the available°uorescent probes for super-resolution imaging, the labeling strategies of carbohydrates, and the recent applications of super-resolution imaging in glycoscience, which will promote the super-resolution imaging technology as a promising tool to provide new insights into the study of glycoscience.

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DNA aptamers bind specifically and selectively to (1→3)-β-d-glucans

Cited by 29 publications

References 19 publications

Selection and Application of ssDNA Aptamers to Detect Active TB from Sputum Samples

Selection and Application of ssDNA Aptamers to Detect Active TB from Sputum Samples

Selection of Peptidoglycan-Specific Aptamers for Bacterial Cells Identification

Super-resolution imaging in glycoscience: New developments and challenges

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