DNA barcode based identification of a suspected tiger skin: A case to resolve mimicry

Khedkar, G.D.; Khedkar, Chandraprakash; Prakash, B. Aditya; Khedkar, Aniket; Haymer, David S.

doi:10.1016/j.fsir.2019.100027

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…The 100% similarity of the forensic samples obtained makes DNA-based identification, especially the CO1 gene, an alternative morphological identification that is limited by incomplete. In addition, identification based on the CO1 gene has also succeeded in uncovering fake tiger skin trading (Khedkar et al 2019). The CO1 gene study has also succeeded in detecting cases of illegal trafficking in Africa (Dalton et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…DNA content provides information on species, individual sex, and geo-location of poached animals with relatively high accuracy (Dalton and Kotze 2011;Karmacharya et al 2018). Mitochondrial DNA, especially the CO1 gene marker has been used for species identification and phylogenetic analysis (Hebert et al 2003;Miller 2007;Bucklin et al 2011;Ashrifurrahman et al 2019;Khedkar et al 2019). In addition, mitochondrial DNA is the basis for the taxonomic determination of tigers (Luo et al 2004;Wilting et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Sumatran tiger identification and phylogenetic analysis based on the CO1 gene: Molecular forensic application

ASHRIFURRAHMAN

SIMAMORA²,

RITONGA³

et al. 2022

Biodiversitas

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Abstract. Ashrifurrahman, Simamora S, Ritonga R, Novarino W, Tjong DH, Rizaldi, Syaifullah, Roesma DI. 2022. Sumatran tiger identification and phylogenetic analysis based on the CO1 gene: molecular forensic application. Biodiversitas 23: 1788-1794. Wild animal hunting, especially in the Sumatran tiger (Panthera tigris sumatrae), has been caused the population decline. Regulation and law enforcement have been implemented even though it does not affect optimal because of the trickery of poachers and illegal traders. Sometimes, the evidence of P. t. sumatrae derivative products, for example, bones, nails, skins, hair, and other body parts, cannot be properly identified to raise the cases. However, genetic markers, such as the CO1 gene, have successfully identified illegal trafficking samples. This study used 20 samples, consisting of seven samples (four preserved hairs, two claws, one bone) that were suspected of P. t. sumatrae collected from illegal wildlife trade cases in West Sumatra, Indonesia. Other thirteen samples were twelve blood and one hair of P. t. sumatrae samples were collected from the Dharmasraya Sumatran Tiger Rehabilitation Center (PR-HSD). All samples were isolated, Polymerase Chain Reaction (PCR), sequenced, and 999 base pairs (bp) of the CO1 gene sequences were analyzed. In addition, National Center for Biotechnology Information (NCBI) data sequences including two P. t. sumatrae sequences, four P. t. altaica sequences, one P. t. amoyensis sequence, one P. t. corbetti sequence, three P. pardus sequences, and one Felis catus sequence were collected for comparison and supporting data. The result confirmed that all samples in this study were P. t. sumatrae. We determined those depending on similarity value which was 99.60%-99.70% with P. tigris reference sequence (NC_010642.1) and 99.90%-100% with P. t. sumatrae (JF357969.1). Phylogenetic analysis supported species identification with average intraspecies sequence divergence was 0 to 0.4% and presented the monophyletic group. This study was the first and most recent report to use seized samples to identify P. t. sumatrae based on the CO1 gene in West Sumatra, Indonesia.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sumatran tiger identification and phylogenetic analysis based on the CO1 gene: Molecular forensic application

ASHRIFURRAHMAN

SIMAMORA²,

RITONGA³

et al. 2022

Biodiversitas

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“…One of these purposes includes the trade of various animal-derived products, such as teeth, claws, or hides, which are highly sought after on the black market. The hides of animals from the genus Panthera, which includes many endangered species, are particularly valuable [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…This can be attributed in part to the substantial demand from Asian communities residing outside Asia [8]. Tanned hides are one of the frequently traded commodities on the black market [7].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Tanning Chemical Agents on DNA Degradation: A Robust Procedure for the Analysis of Tanned Animal Hide—A Pilot Study

Hebenstreitova,

Salaba,

Trubac

et al. 2024

Life

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Illegal wildlife trade is currently on the rise, and it is becoming one of the most lucrative crime sectors. The rarer the species, the higher the demand. Wildlife trade falls under international regulations, such as the CITES convention. Proving that this convention has been violated is a complex process and can be very difficult to do. DNA analysis methods remain (in many cases) the only way to determine whether a certain specimen originated from a protected animal species, a specific individual, or a species in which it is legal to trade. Tanned animal hides are a specific type of specimen. With this type of biological material, obtaining amplifiable DNA is often difficult. This pilot study aimed to map the effect of the chemicals used in the tanning process on the degradation of the DNA yielded from such samples. The DNA was quantified using two different approaches: qPCR and Qubit fluorometry. The degree of DNA fragmentation was assessed by determining the degradation index. The results indicate that reagents containing chromium have the greatest influence on DNA degradation. However, by using the presented protocol, enough amplifiable DNA can be obtained from hides treated with aluminum-based reagents.

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DNA analysis and validation for species identification of seized helmeted hornbill (Rhinoplax vigil) casques

Hatten

Fitriana

Prigge

et al. 2023

Forensic Science International: Animals and Environments

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DNA barcode based identification of a suspected tiger skin: A case to resolve mimicry

Cited by 4 publications

References 19 publications

Sumatran tiger identification and phylogenetic analysis based on the CO1 gene: Molecular forensic application

Sumatran tiger identification and phylogenetic analysis based on the CO1 gene: Molecular forensic application

The Influence of Tanning Chemical Agents on DNA Degradation: A Robust Procedure for the Analysis of Tanned Animal Hide—A Pilot Study

DNA analysis and validation for species identification of seized helmeted hornbill (Rhinoplax vigil) casques

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