DNA barcode data confirm new species and reveal cryptic diversity in ChileanSmicridea(Smicridea) (Trichoptera:Hydropsychidae)

The first record of Tinodes antonioi Botosaneanu & Taticchi-Viganò, 1974 (Insecta, Trichoptera) in Croatia with DNA barcoding and ecological data and notice of biodiversity and distribution of the genus Tinodes in Croatia

“…As in many previous studies (e.g. Pauls et al, 2010;Jackson et al, 2014;Zhou et al, 2007;Zhou, 2009) also in this study, DNA barcoding proves as fast and reliable method for resolving morphological doubts.…”

“…The minimum value of uncorrected pairwise sequence divergences (p-distances) for DNA barcode region between Tinodes species analysed in this study (9%) is higher than minimum values of the interspecific variability usually observed in caddisflies for the mtCOI barcode region (8% Pauls et al, 2010;5.3% Zhou, 2009;8.2% Graf et al, 2015). The maximum observed intraspecific divergence value was 3%.…”

A DNA Barcode Library for Korean Chironomidae (Insecta: Diptera) and Indexes for Defining Barcode Gap

“…With these indexes, we were able to detect 14 cryptic species among 70 controversial species groups (Table 2). In the case of Acentrella turbida of EPCHU and Smicridea annulicornis of CLSMD, the cryptic species had already been described in previous studies (Pauls et al, 2010;Zhou et al, 2009). The remaining 56 controversial species could have been misidentified or may require more sensitive molecular markers for better identification.…”

“…Evaluation of the utility of Index BarcodeGap and Index Cutoff We used 7 insect datasets downloaded from the BOLD system to evaluate the newly developed indexes: Profile 100 Insect Families (SBGB), Ephemeroptera of North America (EPCHU) (Zhou et al, 2009), Trichoptera of Churchill 2002 (PHCAD, TRCAP, MHTRI, DSTRI, and CUCAD) (Zhou et al, 2009), Chilean Smicridea (CLSMD) (Pauls et al, 2010), Caddisflies of New Zealand (NZCAD), Moths of Ontario (PMMO), and BOLD chironomids (GBDPC, COTW, and CAUS). NUMTs were excluded according to the criteria of Song et al (2008).…”

Genetic diversity in the Alpine flatworm <i>Crenobia alpina</i>

“…In general, species are considered cryptic if they have been erroneously classified as a single species because of a lack of distinct morphological features. DNA barcoding has revealed cryptic diversity in a variety of taxa (Nieberding et al, 2005;Bickford et al, 2006;Witt et al, 2006;Lefébure et al, 2007;Pauls et al, 2010). Lefébure et al (2006b) showed that clades of crustaceans diverging by more than 0.16 substitutions per site of the CO I gene as measured by patristic distances are likely to present distinct species.…”