DNA barcoding: a modern age tool for detection of adulteration in food

Nehal, Nazish; Choudhary, Bharti; Nagpure, Anand; Gupta, Rajinder K.

doi:10.1080/07388551.2021.1874279

Cited by 61 publications

(25 citation statements)

References 169 publications

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“…However, the standardization of the methodology to be used for species identification is also pivotal to face the food safety and quality concerns in a global food market. In this regard, the DNA based technologies have proven to be very effective as food fraud detection tools [ 13 , 14 ] and the methodology of DNA barcoding based on the cytochrome oxidase I sequences is the most used one [ 12 , 33 , 34 , 35 , 79 ]. In the last decade new techniques such as Real Time-PCR, Single Nucleotide Polymorphisms (SNPs), Forensically Informative Nucleotide Sequencing (FINS), Loop-mediated isothermal amplification (LAMP), Droplet Digital PCR (ddPCR), High Resolution Melting Analysis (HRMA), Next Generation Sequencing (NGS) metabarcoding, have been proposed by researchers with the aim of optimizing time, costs and effectiveness of species authentication in multi-species fish products [ 80 , 81 , 82 , 83 ].…”

Section: Discussionmentioning

confidence: 99%

COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products

Pappalardo

Giuga

Raffa

et al. 2022

Foods

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The DNA analysis is the best approach to authenticate species in seafood products and to unveil frauds based on species substitution. In this study, a molecular strategy coupling Cytochrome Oxidase I (COI) DNA barcoding with the consolidated methodology of Restriction Fragment Length Polymorphisms (RFLPs), named COIBar-RFLP, was applied for searching pattern of restriction enzyme digestion, useful to discriminate seven different fish species (juveniles of Engraulis encrasicolus and Sardina pilchardus sold in Italy as “bianchetto” and Aphia minuta sold as “rossetto”; icefish Neosalanx tangkahkeii; European perch, Perca fluviatilis and the Nile Perch, Lates niloticus; striped catfish, Pangasianodon hypophthalmus). A total of 30 fresh and frozen samples were processed for DNA barcoding, analyzed against a barcode library of COI sequences retrieved from GenBank, and validated for COIBar–RFLP analysis. Cases of misdescription were detected: 3 samples labeled as “bianchetto” were substituted by N. tangkahkeii (2 samples) and A. minuta (1 sample); 3 samples labeled as “persico reale” (P. fluviatilis) were substituted by L. niloticus and P. hypophthalmus. All species were simultaneously discriminated through the restriction pattern obtained with MspI enzyme. The results highlighted that the COIBar-RFLP could be an effective tool to authenticate fish in seafood products by responding to the emerging interest in molecular identification technologies.

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Section: Discussionmentioning

confidence: 99%

COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products

Pappalardo

Giuga

Raffa

et al. 2022

Foods

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“…DNA barcoding provides a way to confirm the authentication of plants and establish a level of quality assurance within the market [ 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]. Since the first DNA barcoding study [ 60 ], the “animal barcode”, a portion of the mitochondrial gene cytochrome oxidase 1( CO 1), has proved remarkably effective at discriminating among species in diverse groups, such as birds, fishes, and insects [ 60 , 61 ].…”

Section: Introductionmentioning

confidence: 99%

“…The ITS primers, ITS1 and ITS4 [ 86 ] were originally designed for fungi and found useful to detect fungal contamination in herbal plant samples [ 87 , 88 , 89 , 90 ].Sequences of 18S, 5.8S and 26S rDNA are highly conserved from bacteria, fungi and higher plants, enabling the design of the sequence-complemented universal primers for PCR amplification of ITS [ 91 ] across the kingdoms. To improve the quality of ITS sequence information in DNA-barcoding, there are plant-specific ITS primers that can avoid preferential amplification of fungal contaminants or non-plants templates [ 59 , 88 , 92 , 93 ]. Due to the decreased length of the ITS2 sequence (<300 bp), it has been proposed as a suitable for DNA barcoding applications in plants [ 68 , 94 , 95 , 96 , 97 ].There are issues, such as paralogy and polymorphic sites, with the ITS repeats [ 61 , 98 ] that make some taxonomists wary of using them, but for authentication purposes, ITS (and particularly ITS2) have advantages that tend to outweigh these issues.…”

Section: Introductionmentioning

confidence: 99%

Challenges in Medicinal and Aromatic Plants DNA Barcoding—Lessons from the Lamiaceae

Nazar

Howard

Slater

et al. 2022

Plants

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The potential value of DNA barcoding for the identification of medicinal plants and authentication of traded plant materials has been widely recognized; however, a number of challenges remain before DNA methods are fully accepted as an essential quality control method by industry and regulatory authorities. The successes and limitations of conventional DNA barcoding are considered in relation to important members of the Lamiaceae. The mint family (Lamiaceae) contains over one thousand species recorded as having a medicinal use, with many more exploited in food and cosmetics for their aromatic properties. The family is characterized by a diversity of secondary products, most notably the essential oils (EOs) produced in external glandular structures on the aerial parts of the plant that typify well-known plants of the basil (Ocimum), lavender (Lavandula), mint (Mentha), thyme (Thymus), sage (Salvia) and related genera. This complex, species-rich family includes widely cultivated commercial hybrids and endangered wild-harvested traditional medicines, and examples of potential toxic adulterants within the family are explored in detail. The opportunities provided by next generation sequencing technologies to whole plastome barcoding and nuclear genome sequencing are also discussed with relevant examples.

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“…In recent years, remarkable progress has been made towards developing DNA barcoding and DNA metabarcoding methods for food authentication [19][20][21][22][23]. DNA barcoding is based on amplification of short DNA barcode regions, followed by either high resolution melting (HRM) analysis [24,25] or Sanger sequencing [26,27].…”

Section: Introductionmentioning

confidence: 99%

Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding

Preckel

Brünen-Nieweler

Denay³

et al. 2021

Foods

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The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing role in food authentication. In the present study, we investigated the applicability of a DNA metabarcoding method for routine analysis of mammalian and poultry species in food and pet food products. We analyzed a total of 104 samples (25 reference samples, 56 food products and 23 pet food products) by DNA metabarcoding and by using a commercial DNA array and/or by real-time PCR. The qualitative and quantitative results obtained by the DNA metabarcoding method were in line with those obtained by PCR. Results from the independent analysis of a subset of seven reference samples in two laboratories demonstrate the robustness and reproducibility of the DNA metabarcoding method. DNA metabarcoding is particularly suitable for detecting unexpected species ignored by targeted methods such as real-time PCR and can also be an attractive alternative with respect to the expenses as indicated by current data from the cost accounting of the AGES laboratory. Our results for the commercial samples show that in addition to food products, DNA metabarcoding is particularly applicable to pet food products, which frequently contain multiple animal species and are also highly prone to adulteration as indicated by the high portion of analyzed pet food products containing undeclared species.

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DNA barcoding: a modern age tool for detection of adulteration in food

Cited by 61 publications

References 169 publications

COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products

COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products

Challenges in Medicinal and Aromatic Plants DNA Barcoding—Lessons from the Lamiaceae

Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding

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