DNA barcoding: A molecular tool to identify Antarctic marine larvae

Webb, Karen E.; Barnes, David K. A.; Clark, Melody S.; Bowden, David A.

doi:10.1016/j.dsr2.2006.02.013

Cited by 95 publications

(78 citation statements)

References 39 publications

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“…Because of the difficulties associated with the identification of larvae in Antarctica (Webb et al 2006;Bowden et al 2009;Heimeier et al 2010), the equivalent measure of biodiversity is the morphologically based OTU. Simple measures of alpha diversity (OTU richness) and 'species' accumulation curves (Colwell and Coddington 1994) were calculated for larval and embryo OTUs using PRIMER 6.1.11 (Plymouth Marine Laboratory).…”

Section: Methodsmentioning

confidence: 99%

Seasonal patterns in diversity and abundance of the High Antarctic meroplankton: Plankton sampling using a Ross Sea desalination plant

Sewell

Jury

2011

Limnology & Oceanography

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The Antarctic marine environment is thermally stable, yet shows pronounced seasonality in the light : dark cycle, primary productivity, and the extent of sea-ice cover. Thorson's Rule, predicting reduced meroplankton diversity at high latitudes, was based on the perceived difficulties in completing larval development at low temperatures and within the short summer period of phytoplankton production. Yet few studies have addressed seasonal patterns in Antarctic meroplankton diversity and abundance, especially in the High Antarctic. Here we circumvented logistical difficulties in conducting winter plankton sampling by using the 100-mm primary filter on the desalination plant at Scott Base in McMurdo Sound, Ross Sea (77u519S), to sample embryos and larvae. Using samples from an entire calendar year we found no seasonal changes in either embryo or larval operational taxonomic unit (OTU) diversity with season, no seasonal changes in embryo abundance (5000 L 21 ), but a peak in larval abundance during the period of the summer phytoplankton bloom (maximum 295 larvae 5000 L 21 ). While some OTUs showed a strong seasonality, there was also a high diversity of both embryos and larvae during the autumn and winter, and some OTUs were present in the meroplankton year-round. Annelids, molluscs, and echinoderms were dominant members of the Ross Sea meroplankton, showing similar prevalence to that seen at Signy (60u439S) and Adelaide Islands (67u34.59S). The use of the desalination plant primary filter allows plankton samples to be collected year-round, even in the High Antarctic with permanent sea-ice and 4 months of 24-h darkness.

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Section: Methodsmentioning

confidence: 99%

Seasonal patterns in diversity and abundance of the High Antarctic meroplankton: Plankton sampling using a Ross Sea desalination plant

Sewell

Jury

2011

Limnology & Oceanography

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“…red algal species, Saunders, 2005; fungal species, Jaklitsch et al, 2006; and field-collected mosquito specimens, Kumar et al, 2007), especially when size precludes visual identification (i.e. 'unseeable animals', Blaxter et al, 2005;Webb et al, 2006) or if species have polymorphic life cycles and/or exhibit pronounced phenotypic plasticity (e.g. Lamilariales, Lane et al, 2007).…”

Section: -2 When Is the Dna Barcode Useful?mentioning

confidence: 99%

Four years of DNA barcoding: Current advances and prospects

Frézal

Leblois

2008

Infection, Genetics and Evolution

299

202

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“…Also, morphological data may have limited distinguishing power for specific identification of larvae collected in the field, due to phenotypic plasticity in larval morphology as well as variation in life-cycle patterns, for species with complex life cycles (e.g., Boero et al 1992;Minelli and Fusco 2010). By contrast, DNA barcoding is very efficient for this and can be applied to virtually all taxonomic groups and in preserved specimens (Weeb et al 2006;Heimeier et al 2010).…”

Section: Introductionmentioning

confidence: 99%