DNA Barcoding Analysis of Commercial Freshwater Fish Species Cultured in China

Abstract

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Molecular Identification of Several Morphologically Distinct Flowerhorn Fish (Family) Using Mitochondrial <i>COI</i> Gene Marker

“…It is interesting to note that among morphologically distinct samples, especially dubbed as variants of flowerhorn species (Figure 2) such as Zhen Zhu, Kamfa, Parrot, Vieja, Kirin, and Thai Silk, molecular analysis classified them as one clade alongside A. trimaculatus with 0% genetic divergence, while genus with potential parentage showed > 4% divergence as in the Amphilopus genus. Previous study by Wang et al (2015) found that K2P genetic divergence among congeneric farmed freshwater fish averaged 5%, while conspecific divergence averaged 0.34%. The result is similar to this study, with intraspecific divergence is 0% and interspecific divergence being 4.95%.…”

Identification of Acrossocheilus fasciatus (Teleostei: Cyprinidae) from Three Different Phylogeographic Sources using Morphology and COI DNA Barcoding

“…The DNeasy Tissue Kit was used to isolate DNA (Qiagen). Regarding primers for PCR, it was necessary to obtain several COI gene fragments from fish samples; therefore, we used the universal primers COIF: 5-TGTAAAAC GACGGCCAGT CCTGTGGCAATYACDCGCTGAT and COIR: 5-CAGGAAA CAGCTAT-GACNACYTCNGGRT GNCCRAAGAA (Huang et al, 2015). The following reaction conditions were used for polymerase chain reaction (PCR): 10-100 ng of DNA, 2 µl of each dNTP, 1.6 µl of each primer and 0.3 µl of Taq DNA polymerase in the given reaction buffer.…”