DNA barcoding analysis of larval fishes in Peninsular Malaysia

Azmir, Izzati Adilah; Esa, Yuzine; Arshad, Aziz; Salwany, M.Y.I.; Zuraina, Mohd Yusof Farida

doi:10.22438/jeb/41/5(si)/ms_23

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…Similarly, Azmir et al (2020) analyzed fish larvae from Malaysia and found 41 species among the 153 larvae sequenced, also with a great difference between intraspecific average K2P distance (1%) and interspecific divergence (20%). Despite the “gap” observed in their study, five specimens were not identifiable with precision, with low values of identity and similarity for the two databases (GenBank and BOLD—<90%).…”

Section: Discussionmentioning

confidence: 99%

Identification of pre-flexion fish larvae from the western South Atlantic using DNA barcoding and morphological characters

Pozzobon,

Ready,

Di Dario

et al. 2024

PeerJ

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Knowledge on species composition is the first step necessary for the proper conservation and management of biological resources and ecologically relevant species. High species diversity and a lack of diagnostic characters for some groups can impose difficulties for taxonomic identification through traditional methodologies, and ichthyoplankton (fish larvae and eggs) are a good example of such a scenario. With more than 35.000 valid species of fishes worldwide and overall similar anatomies in early developmental stages in closely related groups, fish larvae are often hard to be identified at the species or even more encompassing taxonomic levels. To overcome this situation, molecular techniques have been applied, with different markers tested over the years. Cytochrome c oxidase I (COI) is the most commonly used marker and now has the broadest public reference libraries, providing consistent results for species identification in different metazoan studies. Here we sequenced the mitochondrial COI-5P fragment of 89 fish larvae collected in the Campos Basin, coastal southeastern Brazil, and compared these sequences with references deposited in public databases to obtain taxonomic identifications. Most specimens identified are species of the Blenniiformes, with Parablennius and Labrisomus the most frequently identified genera. Parablennius included two species (P. marmoreus and P. pilicornis), while Labrisomus included three species (L. cricota, L. conditus and L. nuchipinnis). Anatomy of these molecularly identified specimens were then analyzed with the intention of finding anatomical characters that might be diagnostically informative amongst the early development stage (pre-flexion) larvae. Ventral pigmentation patterns are proposed as useful markers to identify Labrisomus species. However, additional specimens are needed to confirm if the character holds stability through the geographic distribution of the species.

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Section: Discussionmentioning

confidence: 99%

Identification of pre-flexion fish larvae from the western South Atlantic using DNA barcoding and morphological characters

Pozzobon,

Ready,

Di Dario

et al. 2024

PeerJ

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“…Several regional DNA barcoding studies have demonstrated its efficacy to delimitate marine fish species, for instance, in Australia 22 , South China Sea 23 , Indian Ocean 24 , and Indo-Pacific coral fishes 25 . DNA barcoding has proven to be a reliable method in detecting cryptic and potentially new fish species 26 – 29 , identifying larval fishes 30 – 33 , or tracing back food origins 34 .…”

Section: Introductionmentioning

confidence: 99%

DNA-based taxonomy of a mangrove-associated community of fishes in Southeast Asia

Abidin

Nor

Lavoué

et al. 2021

Sci Rep

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The Merbok Estuary comprises one of the largest remaining mangrove forests in Peninsular Malaysia. Its value is significant as it provides important services to local and global communities. It also offers a unique opportunity to study the structure and functioning of mangrove ecosystems. However, its biodiversity is still partially inventoried, limiting its research value. A recent checklist based on morphological examination, reported 138 fish species residing, frequenting or subject to entering the Merbok Estuary. In this work, we reassessed the fish diversity of the Merbok Estuary by DNA barcoding 350 specimens assignable to 134 species initially identified based on morphology. Our results consistently revealed the presence of 139 Molecular Operational Taxonomic Units (MOTUs). 123 of them are congruent with morphology-based species delimitation (one species = one MOTU). In two cases, two morphological species share the same MOTU (two species = one MOTU), while we unveiled cryptic diversity (i.e. COI-based genetic variability > 2%) within seven other species (one species = two MOTUs), calling for further taxonomic investigations. This study provides a comprehensive core-list of fish taxa in Merbok Estuary, demonstrating the advantages of combining morphological and molecular evidence to describe diverse but still poorly studied tropical fish communities. It also delivers a large DNA reference collection for brackish fishes occurring in this region which will facilitate further biodiversity-oriented research studies and management activities.

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“…Several regional DNA barcoding studies have demonstrated its e cacy to delimitate sh species, for instance, in Australia 22 , South China Sea 23 , Indian Ocean 24 , and Indo-Paci c coral shes 25 . DNA barcoding has proven to be a reliable method in identifying cryptic and potentially new sh species [26][27][28][29] , marine larval shes [30][31][32][33] , and food origins .…”

Section: Introductionmentioning

confidence: 99%

Assessing fish diversity of Merbok Estuary and adjacent marine waters by DNA taxonomy: towards building comprehensive DNA barcode reference library

Abidin

Nor

Lavoué

et al. 2021

Preprint

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The Merbok Estuary comprises one of the largest remaining mangrove forests in Peninsular Malaysia. Its value is significant as it provides both direct and indirect services to local and global communities. It also offers a unique opportunity to study the structure and functioning of mangrove ecosystems. However, its biodiversity is still partially described, limiting its research value. Recent inventories based on morphological examination, documented 138 fish species residing, frequenting or subject to entering the Merbok Estuary. Using a molecular approach, we assessed the fish diversity of the Merbok Estuary and its adjacent waters in DNA barcoding 350 specimens assignable to 135 species initially identified based on morphology. Our results revealed the presence of 140 MOTUs, 130 of them are congruent with morphology-based species delimitation. In two cases, barcodes did not permit to differentiate between two morphotypes while they unveiled cryptic diversity within six other species, calling for further taxonomic investigations. This study provides a comprehensive core-list of fish taxa in Merbok Estuary, demonstrating the advantages of combining morphological and molecular evidence to describe diverse but still poorly studied tropical fish communities. It also delivers a large DNA reference collection for brackish fishes occurring in this region which will facilitate further biodiversity-oriented research studies and management activities.

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DNA barcoding analysis of larval fishes in Peninsular Malaysia

Cited by 5 publications

References 32 publications

Identification of pre-flexion fish larvae from the western South Atlantic using DNA barcoding and morphological characters

Identification of pre-flexion fish larvae from the western South Atlantic using DNA barcoding and morphological characters

DNA-based taxonomy of a mangrove-associated community of fishes in Southeast Asia

Assessing fish diversity of Merbok Estuary and adjacent marine waters by DNA taxonomy: towards building comprehensive DNA barcode reference library

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