DNA Barcoding and Intra Species Analysis of the Ember Parrot Fish Scarus Rubroviolaceus using mtCO1

Abstract

In this study S.rubroviolaceus form very ancient family group

New report and range extension of smallmouth flounder, Etropus microstomus (Actinopterygii: Carangiformes: Cyclopsettidae), in the Gulf of Mexico

“…Immediately after defrosting, using a sterilized scalpel and forceps a muscle tissue sample (<5 mm) was taken from behind the pectoral fin of each individual and immersed in a 1.5 mL vial with absolute ethanol and kept at -20°C for its preservation (Tiwary et al 2016). The extracted DNA quality was determined by 1.2% agarose gel electrophoresis, and the DNA was used as a template for CO1 amplification.…”

Phylogeographic and Genetic Diversity of Porcellionides Pruinosus and Porcellio Laevis by Using the Mitochondrial Cytochrome C Oxidase Subunit 1 Sequence

“…The mitochondrial genome has been used in wide scale in evolutionary and population genetics studies, by virtue of the high number of copies inside the cell, the ease of isolating it from the nuclear genome, and the small size and rapid accumulation of mutations [22][23][24] . Mitochondrial DNA has several features as introns lack, restricted recombination, has uniparental inheritance (mainly in animal phyla), and rise evolutionary rate [25,26] . The mitochondrial cytochrome c oxidase subunit 1 (CO1) protein-coding gene has been used in a wide scale as a practical and standardized species-level barcode for the majority of the animal kingdom [27] .…”