DNA barcoding and species delimitation of butterflies (Lepidoptera) from Nigeria

Nneji, Lotanna M.; Adeola, Adeniyi C.; Ayoola, Adeola O.; Oladipo, Segun O.; Wang, Yunyu; Malann, Yoila David; Anyaele, Okorie; Nneji, Ifeanyi C.; Rahman, M. M.; Olory, Caroline

doi:10.1007/s11033-020-05984-5

Cited by 13 publications

(7 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nearest neighbour distances and intraspecific distances vary across insect orders. Lepidoptera has been shown to have lower levels of intraspecific variation than Diptera, with average intraspecific distances of 1.08% being found in species from Finland and Austria (Huemer et al, 2014), divergences ranging from 0-5.92% found in species from Nigeria (Nneji et al, 2020), and a threshold of <2% being found appropriate to differentiate most Lepidoptera species in Canada, though 7.7% of species had deeper divergences (Zahiri et al, 2014). The mean divergence between species of Lepidoptera was reported as 5.73% (Pentinsaari, 2016) and between Coleoptera was 11.99% (Pentinsaari et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Building a Collaborative DNA Barcode Library for the Diptera of Churchill, Canada: A Resource for Northern Research

Majoros,

Wang,

Levesque-Beaudin

et al. 2024

Preprint

View full text Add to dashboard Cite

Churchill, Manitoba, Canada is a diverse Subarctic region that has been the focus of DNA barcoding efforts and research for decades. Despite this effort, there are still taxa in this region that are underrepresented in public databases, particularly hyperdiverse insect groups. We present a collaborative large-scale DNA barcode reference library for Diptera based on molecular data and expert taxonomic identification. The reference library contains 16786 specimens sampled around Churchill from 2005 to 2011. Specimens were identified by morphological means, sequenced for the animal barcode marker, and sorted into 2235 Molecular Operational Taxonomic Units (using Barcode Index Numbers: BINs) representing 68 families and 1212 species. Eighty-six species shared a BIN with another species, 98 species were split across several BINs, and 1027 species were assigned to a unique BIN. The intraspecific and nearest neighbour distances varied across families, but most species can be easily distinguished from their nearest neighbours. By combining molecular data with taxonomic expertise, the barcode library provides species-level information for 12612 specimens, supplying future researchers with detailed taxonomic information and the opportunity to perform barcode-based specimen identifications for large-scale studies through sequence matching. Comparison of the Diptera composition of Churchill to other geographic regions reflected likely postglacial colonization northwards as well as a Beringian component. This study provides a publicly available, detailed reference library for use in future research, as well as new insight into the current diversity of Diptera present among the diverse habitats in this Subarctic region.

show abstract

Section: Discussionmentioning

confidence: 99%

Building a Collaborative DNA Barcode Library for the Diptera of Churchill, Canada: A Resource for Northern Research

Majoros,

Wang,

Levesque-Beaudin

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…DNA barcoding is an effective tool for taxonomic delineation of insect species complexes (Brown et al 2012, Tsai et al 2014, Dickey et al 2015, Nneji et al 2020) and thus could also provide useful information for determining the AOC species (Dickey et al 2015, Li et al 2021). In this study, the species boundary of >3% for lepidopteran species was used for taxonomic delineation of the AOC (Hebert et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Genetic differentiation and species diversification of the Adoxophyes orana complex (Lepidoptera: Tortricidae) in East Asia

Tsai,

Shih,

Yeh

et al. 2023

Journal of Economic Entomology

View full text Add to dashboard Cite

The Adoxophyes tea tortrix (Lepidoptera: Tortricidae) is a group of leaf rollers that cause enormous economic losses on tea and apple crops. In East Asia, taxonomic ambiguity of the Adoxophyes orana complex (AOC), which consists of A. orana, A. dubia, A. honmai, and A. paraorana, has persisted for decades because of vague diagnostic characters. In this study, differences in the AOC were examined to improve species identification, determine genetic variations, and develop control strategies. Analyses revealed that A. orana comprised 2 lineages, a widely distributed Palearctic lineage and an East Asian lineage that was nested with other Asian species. Genetic divergence of >3% is proposed to confirm the AOC species that would benefit subsequent taxonomic revision. The monophyletic Taiwanese A. sp. with 2.8–4% from other AOC species appeared to suggest it as an independent taxon, and low interspecific divergence between A. honmai and A. dubia of 0.3% indicated possibility of recent divergence or intraspecific variations. Our result further suggested that the Z9-14:Ac ratio of semiochemicals could be a reference for the reblending of pheromone attractants in Taiwanese tea plantations. Moreover, the AOC species appeared to have a tendency of specific geographic distributions, with A. dubia and A. honmai in Japan and China, A. paraorana in Korea, and A. sp. in Taiwan. Maintaining the unique genetic composition of Adoxophyes species in each geographic region and preventing the possible invasions into those AOC-free countries through the transportation of host plants are essential in managing the AOC in East Asia.

show abstract

“…In line with these morphological differences is the significant genetic distance between the examined specimens, which is 6.1% for the 657 base-pair long COI barcode region. Species delimitation based on pairwise genetic distances of this partial gene region is common practice in modern insect systematics and has been routinely applied for many insect groups such as Lepidoptera (e.g., Hausmann et al 2011;Nneji et al 2020), Coleoptera (e.g., Oba et al 2015;Huang et al 2020), Hymenoptera (e.g., Sheffield et al 2009;Stahlhut et al 2013), and specifically for certain African bees (Bossert et al 2020). While the threshold for delimiting species boundaries is not universal, varies among studies, and is not ultimate proof, a distance of 2-3% is common practice to recognize a barcoding gap (Ratnasingham and Hebert 2013;Hebert et al 2003;and references above).…”

Section: Discussionmentioning

confidence: 99%

A group of two: Scrapter peringueyi is not a synonym of Scrapter heterodoxus (Hymenoptera, Colletidae, Scraptrinae)

Bossert¹,

Noort²

2022

View full text Add to dashboard Cite

Scrapter is a genus of colletid bees with a primary distribution centered in Southern Africa. The genus currently comprises 68 recognized species, which are divided into nine species groups, ranging from one to 29 included species. The Scrapter heterodoxus group is presently considered to be the only monotypic group, because of synonymization of Scrapter heterodoxus with Scrapter peringueyi in a previous revision of the genus. A comparative examination of these two species using both morphological assessment and molecular sequence data from the COI barcode region supported the recognition of S. peringueyi as a valid species, which we accordingly resurrect as the second species of the Scrapter heterodoxus species group. We provide high resolution images of the type specimens for both species and updated diagnoses to enable their separation from all other species of Scrapter.

show abstract

DNA barcoding and species delimitation of butterflies (Lepidoptera) from Nigeria

Cited by 13 publications

References 61 publications

Building a Collaborative DNA Barcode Library for the Diptera of Churchill, Canada: A Resource for Northern Research

Building a Collaborative DNA Barcode Library for the Diptera of Churchill, Canada: A Resource for Northern Research

Genetic differentiation and species diversification of the Adoxophyes orana complex (Lepidoptera: Tortricidae) in East Asia

A group of two: Scrapter peringueyi is not a synonym of Scrapter heterodoxus (Hymenoptera, Colletidae, Scraptrinae)

Contact Info

Product

Resources

About

DNA barcoding and species delimitation of butterflies (Lepidoptera) from Nigeria

Cited by 13 publications

References 61 publications

Building a Collaborative DNA Barcode Library for the Diptera of Churchill, Canada: A Resource for Northern Research

Building a Collaborative DNA Barcode Library for the Diptera of Churchill, Canada: A Resource for Northern Research

Genetic differentiation and species diversification of the Adoxophyes orana complex (Lepidoptera: Tortricidae) in East Asia

﻿A group of two: Scrapter peringueyi is not a synonym of Scrapter heterodoxus (Hymenoptera, Colletidae, Scraptrinae)

Contact Info

Product

Resources

About

A group of two: Scrapter peringueyi is not a synonym of Scrapter heterodoxus (Hymenoptera, Colletidae, Scraptrinae)