DNA barcoding as an approach for species traceability and labeling accuracy of fish fillet products in Thailand

Panprommin, Dutrudi; Manosri, Rittikai

doi:10.1016/j.foodcont.2022.108895

Cited by 19 publications

(11 citation statements)

References 31 publications

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“…In the other nine products analysed (see Table 1 ), only three provided the species name (S. colias ) and the remaining six were labelled both as S. colias and S. japonicus which correspond to the commercial name ‘Lanzardo o Sgombro Occhione’. If we wanted to calculate a misidentification rate only considering the commercial name (“Sgombro”, corresponding to S.scombrus ), 60% of canned products analysed in this study would result as mislabelled, a value higher than those reported in the scientific literature (Neo et al 2022 ; Xing et al 2020 ; Hu et al 2018 ; Panprommin & Manosri 2022 ). Nevertheless, the product “Sgombro” sold canned, due to European Regulation not requiring the scientific name on the label for processed products, can be misinterpreted because it can be related to all mackerel species.…”

Section: Discussionmentioning

confidence: 81%

A Fast and Simple DNA Mini-barcoding and RPA Assay Coupled with Lateral Flow Assay for Fresh and Canned Mackerel Authentication

et al. 2022

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Nowadays, food authentication is more and more required given its relevance in terms of quality and safety. The seafood market is heavily affected by mislabelling and fraudulent substitutions/adulterations, especially for processed food products such as canned food items, due to the loss of morphological features. This study aims to develop new assays based on DNA to identify fresh mackerel ( Scomber spp.) and commercial products. A new primer pair was de novo designed on the 5S rRNA gene and non-transcribed spacer (NTS), identifying a DNA mini-barcoding region suitable for species identification of processed commercial products. Moreover, to offer a fast and low-cost analysis, a new assay based on recombinase polymerase amplification (RPA) was developed for the identification of fresh ‘Sgombro’ ( Scomber scombrus ) and ‘Lanzardo o Occhione’ ( Scomber japonicus and Scomber colias ), coupled with the lateral flow visualisation for the most expensive species ( Scomber scombrus ) identification. This innovative portable assay has great potential for supply chain traceability in the seafood market. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s12161-022-02429-6.

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Section: Discussionmentioning

confidence: 81%

A Fast and Simple DNA Mini-barcoding and RPA Assay Coupled with Lateral Flow Assay for Fresh and Canned Mackerel Authentication

et al. 2022

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“…Studies have showed lack of regulation about common names and scientific Latin names allowed for commercially important species, as well as when the product's label does not include the scientific Latin name of the species used are facilitating factors for species substitution [5,16]. Brazilian legislation on the commercialization of salted cod contemplates these two issues, reinforcing, once again, the need to enforce the provisions of legislation.…”

Section: Species Identificationmentioning

confidence: 99%

DNA Barcoding Identification Exposes Mislabeled Salted Cod (Gadus spp.) Products Retailedat Porto Alegre, Brazil

Noll¹,

Mann²,

Frazzon³

et al. 2023

ABR

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Fish fraud is a worldwide problem, mainly due to the characteristics of the production chain, which includes prior processing of fish on the ship, in addition to the morphological similarities between species. Salted cod, an important commodity imported into Brazil, is one example in which the fish ends up being morphologically mischaracterized due to both salting processes used for its conservation, and, later, due to handling by retailers. Thus, the aim of the present study was to evaluate legal aspects related to commercialization of salted cod, through the evaluation of sale presentation, label information and species involved. Collected samples were handled by retailers previously to sale. DNA barcode sequencing was used as a molecular tool in order to identify species traded under the name "cod". The target was the mitochondrial cytochrome c oxidase gene (COI). Labeling problems were observed in 91% of the analyzed products and species substitution was present in 13% of collected samples. Continuous inspection actions on products sold by retailers are important to reduce the occurrence of fraud in salted fish products.

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“…However, these traditional methods exhibit some disadvantages, which are particularly vulnerable to the influence of identification experience, medicinal material properties, processing methods, and other factors . Recently, with the rapid development of molecular biological technology, DNA barcoding has been widely used for the identification of animal species due to its outstanding specificity and sensitivity, as well as the advantage that it is not affected by the course of working. , …”

Section: Introductionmentioning

confidence: 99%

“…5 Recently, with the rapid development of molecular biological technology, DNA barcoding has been widely used for the identification of animal species due to its outstanding specificity and sensitivity, as well as the advantage that it is not affected by the course of working. 6,7 As is known, the source and grade of Velvet antler slices affect their price and efficacy. 8 From the tip down, Velvet antler is divided into quality grades of wax, powder, and bone slices.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Quality Evaluation of Velvet Antler by DNA Barcoding and Stable Isotope Techniques Combined with Chemometrics

Zeng

Zhou

et al. 2022

ACS Omega

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This study aimed to identify Velvet antler and its counterfeits and to further evaluate their quality. Mitochondrial cytochrome b (Cytb) was used as a target gene to identify Velvet antler samples, and a DNA barcoding method was established for species origin identification in Velvet antlers. After identification, the stable isotope contents and ratios were adopted to evaluate the quality of different specifications of authentic Velvet antler in combination with chemometrics. Two stable isotope contents (C % and N %) and ratios (δ13C and δ15N) in three kinds of Velvet antler slices of different specifications, namely, wax slices, powder slices, and bone slices, were determined. Nine Velvet antler samples sold in the market were identified for label conformity. Only two samples were consistent with the labeled species, and the others were counterfeits. The three slices of Velvet antler of different specifications were clearly distinguished by principal component analysis and hierarchical cluster analysis. Then, the discriminant model of partial least squares discriminant analysis was established, and 100% discrimination accuracy was observed in this model. All the Velvet antler slice samples of different specification samples were grouped clearly according to their sources. In summary, it is feasible for the identification and quality grade evaluation of Velvet antler by DNA barcoding based on mitochondrial Cytb and stable isotope techniques combined with chemometric analysis. The establishment of this method also provided a reference for the evaluation of other animal-derived medicinal materials.

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DNA barcoding as an approach for species traceability and labeling accuracy of fish fillet products in Thailand

Cited by 19 publications

References 31 publications

A Fast and Simple DNA Mini-barcoding and RPA Assay Coupled with Lateral Flow Assay for Fresh and Canned Mackerel Authentication

A Fast and Simple DNA Mini-barcoding and RPA Assay Coupled with Lateral Flow Assay for Fresh and Canned Mackerel Authentication

DNA Barcoding Identification Exposes Mislabeled Salted Cod (Gadus spp.) Products Retailedat Porto Alegre, Brazil

Identification and Quality Evaluation of Velvet Antler by DNA Barcoding and Stable Isotope Techniques Combined with Chemometrics

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