DNA barcoding Cinnamomum osmophloeum Kaneh. Based on the partial non-coding ITS2 region of ribosomal genes

Lee, Shih‐Chieh; Chiou, Shu-Jiau; Yen, Jui-Hung; Lin, Tsai-Yun; Hsieh, Kun-Ting; Yang, Jeng-Chuan

doi:10.38212/2224-6614.2286

Cited by 6 publications

(4 citation statements)

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“…Based on phylogenetic analyses and the GC-MS analysis of essential oils extracted from the leaves, researchers have previously reported at least six chemotypes for the geographical strains of CO in the LHC [20,21,33]. Unfortunately, we were previously unsuccessful in authenticating the chemotype classification for specimens of geographical strains of CO [33]. In the present study, we found that the nucleotide sequence polymorphisms in the ITS2 regions were sufficient to distinguish the geographical strains of CO collected from the LHC (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

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A Useful Technical Application of the Identification of Nucleotide Sequence Polymorphisms and Gene Resources for Cinnamomum osmophloeum Kaneh. (Lauraceae)

Hsu

Lee

2019

Forests

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The plant genus Cinnamomum contains economically important evergreen aromatic trees and shrubs belonging to the laurel family, Lauraceae. Our study tree species Cinnamomum osmophloeum Kaneh. (CO) has high economic value in Taiwan. The present study attempts to identify the gene resources of Cinnamomum osmophloeum Kaneh. by analyzing the nucleotide sequences of the partial noncoding internal transcribed spacer 2 (pITS2) of the ribosomal DNA and the trnL-trnF chloroplast genome. Seventy-three geographical strains of Cinnamomum osmophloeum, preserved in the Lien Hua-Chin Research Center of the Forestry Research Institute and the Hua-Lin Forestry Center of Chinese Culture University, were collected and analyzed by PCR amplification and DNA sequencing to study the genetic diversity and nucleotide sequence polymorphisms of the tested specimens. Our results allowed us to accurately identify the lineage of Cinnamomum osmophloeum and to conclude that the strains belonging to the Lien Hua-Chin Research Center had much higher genetic diversity than those preserved in the Hua-Lin Forestry Center. Multiple sequence alignments demonstrated that the variability of the nucleotide sequence polymorphisms for the pITS2 region was higher than those of the trnL intron and trnL-trnF intergenic spacer (IGS) regions among the 73 tested specimens of Cinnamomum osmophloeum. Cluster analyses, using the neighbor-joining and maximum parsimony methods, for the 73 tested geographical strains of Cinnamomum osmophloeum and species of Cinnamomum registered in the GenBank and EMBL databases were performed to demonstrate the genus and species distribution of the samples. Here, we describe the use of pITS2 polymorphisms as a genetic classifier and report the establishment of a DNA sequence database for CO gene resource identification. The sequence database described in this study can be used to identify CO specimens at the inter- or intraspecies level using pITS2 DNA sequences, which illustrates its value in gene resource identification. Our study results can be used further for correctly identifying the true Cinnamomum osmophloeum Kaneh.

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Section: Discussionmentioning

confidence: 99%

“…We have previously described the DNA barcoding of CO based on the pITS2 region of ribosomal genes [33,34]. Seven representative geographical strains, belonging to six different chemotypes of CO, were used to study genetic diversity.…”

Section: Introductionmentioning

confidence: 99%

A Useful Technical Application of the Identification of Nucleotide Sequence Polymorphisms and Gene Resources for Cinnamomum osmophloeum Kaneh. (Lauraceae)

Hsu

Lee

2019

Forests

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“…Further, Cheng et al, (2004) [19] classified the C. osmophloeum leaf EOs of eight provenances into five chemotypes namely, cinnamaldehyde type, linalool type, camphor type, cinnamaldehyde/cinnamyl acetate type, and mixed type [19]. To continue, based on the abundance the leaf EOs are classified into six chemotypes namely, cinnamaldehyde type, cinnamaldehyde/cinnamyl acetate type, cinnamyl acetate type, linalool type, camphor type, and mixed type [27,28]. It is identified that the EOs and key constituents from the leaves of two C. osmophloeum clones are belongs to two different chemotypes, which are classified as the cinnamaldehyde type and camphor type [29].…”

Section: Essential Oil Componentsmentioning

confidence: 99%

A Comprehensive Review on Phytochemical, Pharmacological and Future Prospective of Dietary Medicinal Plant Cinnamomum osmophloeum Kanehira

2022

Curr Res Cmpl Alt Med

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Background:The plants belonging to the genus Cinnamomum traditionally used as an ethnomedicine in Asia, Europe, and North America. Cinnamomum osmophloeum Kanehira, is an endemic and economic medicinal plant of Taiwan. It is traditionally used as an antibacterial, antifungal, anti-termite, antidiabetic, anti-hyperuricemia, antiinflammatory, and antioxidant agent. Despite these attributes, C. osmophloeum is not enough explored scientifically. This review updated the essential oils and other secondary metabolites, and the pharmacological activities of C. osmophloeum, as well as its other economic benefits. Methods: The information in the review is extracted from the major scientific databases, such as PubMed, BioMed Central, Google scholar, Elsevier, ACS publications, MDPI, Taylor and Francis, Wiley Online Library, Scopus, Springer, and Web of Science, using journals, dissertations, books and/or chapters, and conference proceedings. Results: Various secondary metabolites including essential oil components, flavonoids, lignans, and benzenoids are reported from the extracts of C. osmophloeum. The review established a wide range of pharmacological properties including, antibacterial, antidiabetic, anti-fungal, anti-inflammatory, antioxidant, antitermitic, anti-tyrosinase, anti-xanthine oxidase, anxiolytic, cytotoxic, hepatoprotective, and mosquito larvicidal properties of extracts, as well as essential oils and other secondary metabolites of C. osmophloeum. Conclusions: The present review provides a scientific basis for future studies and necessary information for the development of C. osmophloeum based therapeutic agents.

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“…However, both studies used good-quality and -quantity DNA from leaves and primer pairs that yielded 193 bp (ITS2) and 429 bp ( trnL - trnF ) amplicons for species identification. DNA barcoding was proposed as an authentication method based on the use of several DNA regions [ 48 , 49 , 50 , 51 ]. Methods for DNA isolation have been developed specifically for Cinnamomum species based on leaves [ 52 , 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

Nuclear Magnetic Resonance Fingerprints and Mini DNA Markers for the Authentication of Cinnamon Species Ingredients Used in Food and Natural Health Products

Ragupathy,

Thirugnanasambandam,

Vinayagam

et al. 2024

Plants

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Cinnamomum verum (syn C. zeylanicum) is considered ‘true’ cinnamon. However, it is reported that less expensive sources of cinnamon from C. cassia (syn C. aromaticum), C. loureiroi, and C. burmannii (toxic coumarin) may be used in the place of C. verum. We lack the quality assurance tools that are required to differentiate C. verum from other cinnamon species when verifying that the correct species is sourced from ingredient suppliers. The current research on cinnamon species authentication using DNA tools is limited to a few species and the use of high-quality DNA extracted from raw leaf materials. The cinnamon bark traded in the supply chain contains much less DNA and poorer-quality DNA than leaves. Our research advances DNA methods to authenticate cinnamon, as we utilized full-length chloroplast genomes via a genome skimming approach for C. burmannii and C. cassia to facilitate the design of optimal mini DNA markers. Furthermore, we developed and validated the use of NMR fingerprints for several commercial cinnamon species, including the quantification of 16 molecules. NMR fingerprints provided additional data that were useful for quality assessment in cinnamon extract powders and product consistency. Both the new mini DNA markers and NMR fingerprints were tested on commercial cinnamon products.

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DNA barcoding Cinnamomum osmophloeum Kaneh. Based on the partial non-coding ITS2 region of ribosomal genes

Cited by 6 publications

References 0 publications

A Useful Technical Application of the Identification of Nucleotide Sequence Polymorphisms and Gene Resources for Cinnamomum osmophloeum Kaneh. (Lauraceae)

A Useful Technical Application of the Identification of Nucleotide Sequence Polymorphisms and Gene Resources for Cinnamomum osmophloeum Kaneh. (Lauraceae)

A Comprehensive Review on Phytochemical, Pharmacological and Future Prospective of Dietary Medicinal Plant Cinnamomum osmophloeum Kanehira

Nuclear Magnetic Resonance Fingerprints and Mini DNA Markers for the Authentication of Cinnamon Species Ingredients Used in Food and Natural Health Products

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