2020
DOI: 10.1016/j.jarmap.2020.100269
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DNA barcoding coupled with secondary structure information enhances Achyranthes species resolution

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Cited by 1 publication
(2 citation statements)
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“…According to a previous study, the features of the ITS2 region are conserved, which in turn provides molecular and morphological features that could be used to improve the resolution of species identification [ 40 ]. The usefulness of ITS2 sequences and the ITS2 secondary structure as genetic markers has been reported for several medicinal plant species [ 20 , 41 , 42 ]. Regarding the ITS2 secondary structure, the conservative standard structural model contained the feature of a “loop” with four “helices.” Among the four “helices,” helix I and helix IV had the most variations, helix II and helix III were more conserved, helix II was shorter with a pyrimidine–pyrimidine mismatch “bridge,” while helix III was the longest with many branches [ 30 , 43 , 44 ].…”
Section: Discussionmentioning
confidence: 99%
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“…According to a previous study, the features of the ITS2 region are conserved, which in turn provides molecular and morphological features that could be used to improve the resolution of species identification [ 40 ]. The usefulness of ITS2 sequences and the ITS2 secondary structure as genetic markers has been reported for several medicinal plant species [ 20 , 41 , 42 ]. Regarding the ITS2 secondary structure, the conservative standard structural model contained the feature of a “loop” with four “helices.” Among the four “helices,” helix I and helix IV had the most variations, helix II and helix III were more conserved, helix II was shorter with a pyrimidine–pyrimidine mismatch “bridge,” while helix III was the longest with many branches [ 30 , 43 , 44 ].…”
Section: Discussionmentioning
confidence: 99%
“…According to the previous literature, trn L and ITS DNA barcodes can be used to distinguish between the Nepenthes species based on their geographical origin area [ 17 ]. Meanwhile, other studies concluded that RNA secondary structure prediction is an advanced tool for species discrimination [ 18 ], and the integration of secondary structure information in species identification can significantly improve its accuracy for other plant species [ 18 , 19 , 20 ]. However, there are not many reports on how ITS1 and ITS2 secondary structure predictions can be used together to differentiate between species.…”
Section: Introductionmentioning
confidence: 99%