DNA barcoding: How many earthworm species are there in the south of West Siberia?

Shekhovtsov, Sergei V.; Bazarova, N. E.; Берман, Д. И.; Булахова, Н. А.; Голованова, Е. В.; Konyaev, Sergey; Krugova, T.M.; Lyubechanskii, I. I.; Peltek, S. E.

doi:10.1134/s2079059717010130

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…А определение неполовозрелых и поврежденных особей часто невозможно, из-за чего они не используются в исследованиях [Richard et al, 2010]. Поэтому молекулярные подходы, такие, как анализ ДНК, стали использоваться как альтернативный инструмент для идентификации дождевых червей [Shekhovtsov et al, 2017] или разделения морфологически схожих видов [Shekhovtsov et al, 2016]. Несмотря на технологическое развитие и снижение стоимости оборудования и реактивов для проведения секвенирования [Gautier et al, 2018;Srivathsan et al, 2019], анализ ДНК редко применяется в исследованиях из-за высокой стоимости, поэтому существует необходимость использовать недорогие и доступные методы идентификации таксонов (видов) дождевых червей с помощью более дешевых альтернативных молекулярных подходов [Vaupel, Hommel, Beule, 2022] или метаболомики, которая основана на методах аналитической химии, анализирующей глобальный метаболический профиль объекта [Rodríguez-Fernández et al, 2010].…”

Section: Introductionunclassified

Investigation of differences in the IR spectra of earthworms as a possible way of their taxonomic identification

Kniazev,

Babiy,

Golovanova

et al. 2023

Вест. ПГУ. Биол.

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IR spectrometry is successfully used for the taxonomic identification of various groups of invertebrates: butterflies, flies, grasshoppers, mosquitoes, weevils, and termites. Therefore, the purpose of this study was to assess the fundamental possibility of taxonomic identification of earthworms based on the study of the functional-group composition of their bodies by the method of IR-Fourier spectroscopy. Seven species of earthworms from five genera were the objects of the study: Aporrectodea caliginosa, Eisenia fetida, Eisenia nordenskioldi, Lumbricus rubellus, Rhiphaeodrilus diplotetrathecus, Octolasion lacteum, Eisenia ventripapillata. IR absorption spectra were registered on an FT-801 IR-Fourier spectrometer in the range of 500–4000 cm–1. The position, intensity, and area of the absorption bands were determined on all spectra. The maximum differences between individual species in intensity and position of protein and nucleic acid absorption bands were established. Using the principal component method, it was shown that the classification was based on the characteristics of the absorption bands of stretching and bending vibrations of methyl and methylene groups, as well as amide groups in the structure of worms. At the same time, the division of earthworms into subgroups with similar spectral characteristics could be explained by several reasons: their origin, kinship, and the influence of the environment. The data obtained during the study confirm the possibility of using IR spectrometry for the identification of earthworms.

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Section: Introductionunclassified

Investigation of differences in the IR spectra of earthworms as a possible way of their taxonomic identification

Kniazev,

Babiy,

Golovanova

et al. 2023

Вест. ПГУ. Биол.

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“…DNA sequence data (Blakemore, 2013 ; Shekhovtsov et al, 2013 , 2015 , 2016a , b , 2017b , 2018a , b ; Hong and Csuzdi, 2016 ) demonstrated that genetic diversity within E. nordenskioldi is extremely high. Different genetic lineages of E. nordenskioldi can differ by as much as 22% of nucleotide substitutions in the mitochondrial COI gene (Shekhovtsov et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data

et al. 2020

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Eisenia nordenskioldi (Eisen, 1879) is the only autochthonous Siberian earthworm with a large distribution that ranges from tundra to steppe and broadleaved forests. This species has a very high morphological, ecological, karyological, and genetic diversity, so it was proposed that E. nordenskioldi should be split into several species. However, the phylogeny of the complex was unclear due to the low resolution of the methods used and the high diversity that should have been taken into account. We investigated this question by (1) studying the diversity of the COI gene of E. nordenskioldi throughout its range and (2) sequencing transcriptomes of different genetic lineages to infer its phylogeny. We found that E. nordenskioldi is monophyletic and is split into two clades. The first one includes the pigmented genetic lineages widespread in the northern and western parts of the distribution, and the second one originating from the southern and southeastern part of the species' range and representing both pigmented and non-pigmented forms. We propose to split the E. nordenskioldi complex into two species, E. nordenskioldi and Eisenia sp. 1 (aff. E. nordenskioldi), corresponding to these two clades. The currently recognized non-pigmented subspecies E. n. pallida will be abolished as a polyphyletic and thus a non-natural taxon, while Eisenia sp. 1 will be expanded to include several lineages earlier recognized as E. n. nordenskioldi and E. n. pallida.

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Molecular differentiation of epigeic and anceic forms of Drawida ghilarovi Gates, 1969 (Moniligastridae, Clitellata) in the Russian Far East: Sequence data of two mitochondrial genes

Ganin

Atopkin

2018

European Journal of Soil Biology

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DNA barcoding: How many earthworm species are there in the south of West Siberia?

Cited by 7 publications

References 22 publications

Investigation of differences in the IR spectra of earthworms as a possible way of their taxonomic identification

Investigation of differences in the IR spectra of earthworms as a possible way of their taxonomic identification

Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data

Molecular differentiation of epigeic and anceic forms of Drawida ghilarovi Gates, 1969 (Moniligastridae, Clitellata) in the Russian Far East: Sequence data of two mitochondrial genes

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