DNA barcoding of some medicinal plants, family Labiatae

Elsherbeny, Esraa

doi:10.5455/egyjebb.20160810065917

Cited by 1 publication

(2 citation statements)

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“…It is worth noticing the analysis of DNA sequences for the rbcL molecular marker and the species blasted to M. aschersonii. Two of the accessions characterized as M. vulgare (U28875 and HM590056) revealed high dissimilarities (18 bp) compared to M. aschersonii but also to ten other rbcL DNA sequences of M. vulgare (14 bp), and therefore should be checked and experimented again [4]. The matK and trnH/psbA molecular markers have been widely used for many plants [56], and they have also been used for the identification of some Marrubium species [6,57].…”

Section: Macro-and Micronutrient Contentmentioning

confidence: 99%

“…Previous research has been conducted very early in attempts to characterize the Tribe Marrubieae using the chloroplast rbcL gene [3]. Single nucleotide polymorphisms (SNPs) have been extensively used for three species of Marrubium using the ITS and rbcL molecular markers [4]; the chloroplast markers matK, trnL, trnL-trnF, and rps16 for five species of Marrubium [5,6]; and finally the most complete phylogenetic approach using 36 accessions representing 27 species of Marrubium applying the ITS of nrDNA and rpl32-trnL, trnL, trnL-trnF and rps16 of cpDNA molecular markers [2]. There is also a RAPD profiling for M. alysson L. and M. vulgare L. [7] and ISSRs for five species of Marrubium [8] reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

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Facilitating Conservation and Bridging Gaps for the Sustainable Exploitation of the Tunisian Local Endemic Plant Marrubium aschersonii (Lamiaceae)

et al. 2022

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In the frame of conservation and sustainable utilization of neglected and underutilized phytogenetic resources, this study produced for the first time a detailed ecological profiling for the local Tunisian endemic Marrubium ascheronii (Lamiaceae) using Geographical Information Systems and open-source data. This profile was used to illustrate the abiotic environmental conditions of its wild habitats; the profile facilitated the examination of the effect of temperature on its seed germination and may inform species-specific guidelines for its cultivation in man-made environments. With effective seed propagation firstly reported herein (68.75% at 20 °C), species-specific in situ conservation efforts and ex situ conservation or sustainable exploitation strategies were enabled for M. aschersonii. The first-reported molecular authentication (DNA barcoding) of M. aschersonii may facilitate its traceability, allowing for product design. This study also reports for the first time the effects of chemical and integrated nutrient management (INM) fertilizers on the growth and pilot cultivation of M. aschersonii seedlings, with the latter being advantageous. This multidisciplinary approach has bridged important research gaps that hindered the conservation efforts and/or the sustainable exploitation for this local endemic plant of Tunisia. Based on all the above, we re-evaluated and updated the feasibility and readiness timescale for sustainable exploitation of M. aschersonii in the medicinal-cosmetic, agro-alimentary and ornamental-horticultural sectors.

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