DNA barcoding of Stearibia nigriceps (Meigen) and Piophila casei (Linnaeus) (Diptera: Piophilidae) from Algeria and the first African report of Stearibia nigriceps

Taleb, Meriem; Tail, Ghania; Açikgöz, Halide Nihal

doi:10.1007/s00414-019-02223-w

Cited by 6 publications

(5 citation statements)

References 48 publications

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“…Using the BLAST tool, we succeeded in identifying 172 of the 174 sequenced samples as Stearibia nigriceps (Meigen), with an intraspecific p-distance of 0.3%-1.5%. This is in line with the average intraspecific distance of 1.12% (with a maximum distance of 4.42%) observed by Zajac et al [11] between specimens of S. nigriceps obtained from five European countries, as well as the intraspecific distances of less than 3% observed by Taleb et al [18] between Algerian specimens of the same species. In the present study, we distinguished six haplogroups for the South Korean specimens of S. nigriceps, with percentage intragroup distances ranging from 0.48% to 1.055% (Table 3) and intergroup distances ranging from 0.747% to 1.833% (Table 4).…”

Section: Re Sults and Discussionsupporting

confidence: 91%

“…This is in line with the average intraspecific distance of 1.12% (with a maximum distance of 4.42%) observed by Zajac et al [11] between specimens of S . nigriceps obtained from five European countries, as well as the intraspecific distances of less than 3% observed by Taleb et al [18] between Algerian specimens of the same species. In the present study, we distinguished six haplogroups for the South Korean specimens of S .…”

Section: Resultsmentioning

confidence: 70%

“…In the present study, we distinguished six haplogroups for the South Korean specimens of S. nigriceps, with percentage intragroup distances ranging from 0.48% to 1.055% (Table 3) and intergroup distances ranging from 0.747% to 1.833% (Table 4). Stearibia nigriceps (sometimes referred to under the invalid names of Piophila nigriceps or Piophila foveolata) is one of the most widely distributed Piophilidae species, occurring through the Holarctic, Oriental, and Neotropical regions [4,5,18]. This wide geographical distribution can probably be attributed to the eusynanthropic habits of this species [19], which may have accompanied humans to different parts of the world.…”

Section: Re Sults and Discussionmentioning

confidence: 99%

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First report of the forensically important fly, Stearibia nigriceps (Diptera: Piophilidae) in South Korea: Confirmation of specimens from human corpses based on cytochrome c oxidase subunit I barcodes

Kim

Martín‐Vega

Shin

et al. 2021

Journal of Forensic Sciences

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Piophilidae are a relatively small family of Diptera that is frequently associated with cadavers at advanced stages of decomposition and are, therefore, considered potentially useful forensic indicators. However, their use in forensic investigations is typically hampered by a deficiency in reliable identification tools. This is particularly evident in countries such as South Korea, where forensic entomology is still in its infancy and the diversity of forensically relevant insect taxa remains largely undocumented. In the present study, we used cytochrome c oxidase subunit I (COI) barcodes to identify samples of piophilid larvae collected during medicolegal investigations performed in South Korea. A total of 174 COI sequences were obtained and have been made publicly available, thus augmenting the reference barcode library for forensically important Piophilidae species. Of the 174 sequenced samples, 172 were identified as Stearibia nigriceps (Meigen), whereas the two remaining samples may represent a previously unsequenced piophilid species. Stearibia nigriceps is recorded from South Korea for the first time, and our results suggest that it might be a particularly relevant forensic indicator in certain case types and scenarios in that country. The findings of this study highlight the utility of COI barcodes for achieving accurate identification of entomological samples, even by non-specialist forensic practitioners. They also contribute to the further development and consolidation of forensic entomology in South Korea and eastern Asia. K E Y W O R D S cytochrome c oxidase subunit I, DNA barcoding, forensic entomology, Piophilidae, Stearibia nigriceps, necrophagous insect, range extension Highlights• COI barcodes were used to identify piophilid larvae collected from human cadavers.• Of the 174 sequenced samples, 172 were identified as Stearibia nigriceps (Meigen).• Two samples may represent a previously unsequenced species.• Both piophilid species are recorded from South Korea for the first time.• Our findings will contribute to the development of forensic entomology in South Korea. | 1539 KIM et al.

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Section: Re Sults and Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 70%

Section: Re Sults and Discussionmentioning

confidence: 99%

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First report of the forensically important fly, Stearibia nigriceps (Diptera: Piophilidae) in South Korea: Confirmation of specimens from human corpses based on cytochrome c oxidase subunit I barcodes

Kim

Martín‐Vega

Shin

et al. 2021

Journal of Forensic Sciences

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“…According to previous studies, the intraspecific divergences are rarely greater than 2% and most are less than 1% [ 66 ],. This was also verified in studies of several insect groups, such as Lepidoptera [ 67 ], Piophilidae [ 68 ], Blattidae [ 69 ], Liposcelididae [ 70 ], etc. The recent study of leafhoppers based on COX1 gene fragments also showed that the intraspecific divergence was less than 1% [ 71 ].…”

Section: Resultsmentioning

confidence: 58%

Comparative Analysis of Mitochondrial Genomes among Twelve Sibling Species of the Genus Atkinsoniella Distant, 1908 (Hemiptera: Cicadellidae: Cicadellinae) and Phylogenetic Analysis

Jiang

et al. 2022

Insects

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The herbivorous leafhopper genus Atkinsoniella Distant, 1908 (Hemiptera: Cicadellidae: Cicadellinae), a large genus of subfamily Cicadellinae, consists of 98 valid species worldwide and 88 species recorded in China. Some species of the genus are very similar in morphological characteristics, so they are difficult to identify accurately. In this study, 12 mitochondrial genomes of Atkinsoniella species with similar morphological characteristics were first obtained through high-throughput sequencing, which featured a typical circular molecule of 15,034–15,988 bp in length. The arrangement and orientation of 37 genes were identical to those of typical Cicadellidae mitogenomes. The phylogenetic relationship within the subfamily Cicadellinae was reconstructed using maximum-likelihood (ML) and Bayesian inference (BI) methods based on three concatenated datasets. The topological structures of the six obtained phylogenetic trees were highly consistent. The results suggested that Atkinsoniella was recovered as a monophyletic group and emerged as a sister group with the monophyletic clade of Bothrogonia, Paracrocampsa (part), and Draeculacephala (part). The branches of the 12 newly sequenced species were clearly separated, with most nodes receiving strong support in all analyses. In addition, the key to the 12 Atkinsoniella species was provided to identify species according to morphological characteristics. This study further promotes research on the classification, genetics, evolution, and phylogeny of the genus Atkinsoniella and subfamily Cicadellinae.

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“…Stevens, et al [ 53 ] have also reported the presence of intraspecific divergence and geographical disparities between blow fly (Calliphoridae) populations. However, it is important to use multiple genetic markers and a large number of specimens to thoroughly examine the population assembly and the accurate genetic divergence within a species [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Molecular identification of the potentially forensically relevant cluster fliesPollenia rudis(Fabricius) andPollenia vagabunda(Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

Taleb

Tail

Açikgöz

2021

Forensic Sciences Research

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Cluster flies are represented by the genus Pollenia Robineau-Desvoidy, 1830 of the family Polleniidae Brauer and Bergenstamm, 1889. Their larvae are known to be internal parasites or predators of earthworms. Herein, we report for the first time the occurrence of the cluster flies Pollenia rudis Fabricius, 1794 and Pollenia vagabunda (Meigen, 1826) (Diptera: Polleniidae) on carcasses in Algeria and identify them through DNA barcoding. A region of the mitochondrial cytochrome c oxidase I gene ( COI ) was amplified and sequenced. Genetic distances were determined. A phylogenetic tree was constructed with the maximum parsimony method using 10 000 bootstrap replicates. A total number of 157 adults of P. rudis were collected together with 325 adults of Pollenia vagabunda . The occurrence of Pollenia on animal carcasses does not seem to be correlated with a particular stage of decomposition. All the sequences were correctly identified using the BLASTn tool from the GenBank database and the BOLD identification engine. Intra- and interspecific sequence divergence values were less than 1% and greater than 3%, respectively. COI barcodes obtained from this study were robust enough to identify and distinguish unambiguously between P. rudis and P. vagabunda . In the tree-based analysis, the cluster flies were all assigned to their respective species separately from each other confirming the morphological identification. These results provide DNA barcodes that contribute to the growth of reference databases and allow fast and accurate identification.

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DNA barcoding of Stearibia nigriceps (Meigen) and Piophila casei (Linnaeus) (Diptera: Piophilidae) from Algeria and the first African report of Stearibia nigriceps

Cited by 6 publications

References 48 publications

First report of the forensically important fly, Stearibia nigriceps (Diptera: Piophilidae) in South Korea: Confirmation of specimens from human corpses based on cytochrome c oxidase subunit I barcodes

First report of the forensically important fly, Stearibia nigriceps (Diptera: Piophilidae) in South Korea: Confirmation of specimens from human corpses based on cytochrome c oxidase subunit I barcodes

Comparative Analysis of Mitochondrial Genomes among Twelve Sibling Species of the Genus Atkinsoniella Distant, 1908 (Hemiptera: Cicadellidae: Cicadellinae) and Phylogenetic Analysis

Molecular identification of the potentially forensically relevant cluster fliesPollenia rudis(Fabricius) andPollenia vagabunda(Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

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