DNA barcoding reveals a new record of Potamogeton distinctus (Potamogetonaceae) and its natural hybrids, P. distinctus × P. nodosus and P. distinctus × P. wrightii (P. ×malainoides) from Myanmar

Yu, I.; Tanaka, Norio; Pooma, Rachun; Tanaka, Nobuyuki

doi:10.3897/bdj.2.e1073

Cited by 7 publications

(11 citation statements)

References 31 publications

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“…DNA was extracted with the standard CBG protocols (Ivanova et al, 2008(Ivanova et al, , 2011. Two highly variable DNA regions were selected to design eDNA markers: the plastid-encoded intergenic spacer atpB-rbcL (Ito et al, 2014) and the nuclear-encoded ribosomal internal transcribed spacer ITS2 (China Plant BOL Group, 2011). The primers for these markers, specific for Potamogetonaceae, were designed to recover all species selected for the local reference library that generated amplicons suitable for eDNA detection.…”

Section: Selecting Edna Markers and Creating A Dna Reference Librarymentioning

confidence: 99%

Finding the pond through the weeds: eDNA reveals underestimated diversity of pondweeds

2018

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Premise of the StudyThe detection of environmental DNA (eDNA) using high‐throughput sequencing has rapidly emerged as a method to detect organisms from environmental samples. However, eDNA studies of aquatic biomes have focused on surveillance of animal species with less emphasis on plants. Pondweeds are important bioindicators of freshwater ecosystems, although their diversity is underestimated due to difficulties in morphological identification and monitoring.MethodsA protocol was developed to detect pondweeds in water samples using atpB‐rbcL and ITS2 markers. The water samples were collected from the Grand River within the rare Charitable Research Reserve, Ontario (RARE). Short fragments were amplified using primers targeting pondweeds, sequenced on an Ion Torrent Personal Genome Machine, and assigned to the taxonomy using a local DNA reference library and GenBank.ResultsWe detected two species earlier documented at the experimental site during ecological surveys (Potamogeton crispus and Stuckenia pectinata) and three species new to the RARE checklist (P. foliosus, S. filiformis, and Zannichellia palustris).DiscussionOur targeted approach to track the species composition of pondweeds in freshwater ecosystems revealed underestimation of their diversity. This result suggests that eDNA is an effective tool for monitoring plant diversity in aquatic habitats.

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Section: Selecting Edna Markers and Creating A Dna Reference Librarymentioning

confidence: 99%

Finding the pond through the weeds: eDNA reveals underestimated diversity of pondweeds

2018

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“…Although hybrids in Potamogetonaceae were first recognized already in the late 19th century (see Kaplan & al., 2009 for a brief review), molecular evidence became available only during the past decades by using isozyme electrophoresis (e.g., Hollingsworth & al., 1995; Fant & al., 2001; Iida & Kadono, 2002; Kaplan & al., 2002; Kaplan & Wolff, 2004; Kaplan, 2007; Bobrov & Sinjushin, 2008) and DNA‐based techniques (e.g., King & al., 2001; Fant & al., 2003; Kaplan & Fehrer, 2004, 2009, 2011, 2013; Ito & al., 2007, 2014; Du & al., 2009, 2010; Les & al., 2009; Zalewska‐Gałosz & al., 2009, 2010, 2018; Kaplan & al., 2011, 2018; Aykurt & al., 2017; Iida & al., 2018). While these studies focused on hybrids, a few of them performed also phylogenetic analyses (e.g., Les & al., 2009; Kaplan & Fehrer, 2011; Ito & al., 2014; Kaplan & al., 2018; Zalewska‐Gałosz & al., 2018), but these include only taxa relevant for the identification of hybrids. Several studies focused on the phylogeny of Potamogetonaceae (Iida & al., 2004; Lindqvist & al., 2006; Wang & al., 2007; Zhang & al., 2008; Ito & Tanaka, 2013).…”

Section: Introductionmentioning

confidence: 99%

Taxonomic treatment and phylogenetic analysis of the family Potamogetonaceae in Turkey

Aykurt

Fehrer

Yol

et al. 2020

TAXON

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The family Potamogetonaceae has been taxonomically re-evaluated in Turkey, employing traditional morphological as well as molecular approaches (rbcL, ITS). Our fieldwork, herbarium studies and molecular analyses proved the existence of 22 taxa, of which 18 belong to Potamogeton (including 4 interspecific hybrids), 3 to Stuckenia and 1 to monotypic Groenlandia. Morphological re-descriptions of the recognized taxa were prepared, and the information concerning their distribution in Turkey was refined, based on plant material from extensive fieldwork and on specimens stored in herbaria that were previously not examined. Additionally, new identification keys to genera and species, and distribution maps of the species were prepared. Phylogenetic relationships and intraspecific variations were assessed by including samples from other regions. The status of the Zannichelliaceae was investigated using nuclear and chloroplast DNA markers. Our results from ITS sequence divergence corroborate the separation of the two families noted by some authors, and are in accordance with the substantial morphological differences between them. Relatively large genetic distance and non-monophyly indicate that two genotypes of P. gramineus constitute cryptic species, for which the Turkish localities expand upon the previously known distribution areas. We identified P. schweinfurthii as a new species for Turkey and report P. ×angustifolius for the first time for this country. Weak morphological differentiation and high sequence similarity did not permit reliable differentiation between the closely related Stuckenia amblyphylla and S. filiformis.

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“…Гибридизация особенно широко распространена среди водных и прибрежно-водных растений (Papchenkov, 2007;Iida et al, 2013). Большое число гибридов отмечено в составе родов Potamogeton L. (Papchenkov, 2007;Kaplan, 2007;Gałosz and Ronikier, 2012;Kaplan and Fehrer, 2013;Iida et al, 2013;Ito et al, 2014;Yang et al, 2016), Nuphar Smith (Padget et al, 1998Arrigo et al, 2016), Nymphaea L. (Nierbauer et al, 2014;Borsch et al, 2014), Carex L. (Wiecław and Koopman, 2013;Więcław and Wilhelm, 2014;Pedersen et al, 2016), Ranunculus L. (Zalewska-Gałosz et al, 2014;Bobrov et al, 2015), Typha L. (Ball and Freeland, 2013;Freeland et al, 2013;Kapitonova et al, 2015). Не явился исключением и род Sparganium L., высокая гибридогенная активность которого отмечена многими исследователями (Rothert, 1910;Nicholls, 1986, 1987;Sulman et al, 2013;Ito et al, 2016).…”

Section: Introductionunclassified

Morphology and ecological characteristics of Sparganium × longifolium (Typhaceae) in the Central part of European Russia

et al. 2017

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The increasing impact of anthropogenic factors and climate change affect the growth of a number of taxa of hybrid nature. These taxa are widespread among various taxonomic groups of aquatic and semi-aquatic plants. The genus Sparganium L. В is not an exception. In that regard, the aim of this study is to conduct biomorphological investigation of Sparganium × longifolium Turcz. ex Ledeb., evaluate qualitative and quantitative criteria for the hybrid similarities and differences with its parental species, as well as to analyze data on its habitat characteristics. Samples were collected in 2014-2016 from waterbodies in European Russia (Tver and Yaroslavl oblasts). In the study on biomorphology of S. × longifolium we used live and fixed materials, as well as herbarium funds of IBIW, MXA and MW. To establish and specify taxonomic features of the hybrid under study, indicating to its similarity with a certain ancestral species, our data on the morphology and ecology of S. emersum Rehm. and S. gramineum Georgi. are used. During field studies, the type of a water object where the hybrid was detected, ecological characteristics of its habitat (type of soil, depth, water temperature and pH) are determined; the list of taxa which enter into the cenosis composition is compiled. The biomorphological investigation of S. × longifolium shows that by life form this hybrid, as well as its parental species, is a vegetative-mobile evidently-polycentric annual or biennial plant of vegetative origin with a racemose root system. The following should be attributed to the characteristic features justifying the hybrid origin of S. × longifolium: 1) a wider, slightly carinated lamina (as in S. emersum); 2) a branched inflorescence (as in S. gramineum); 3) the lower covering leaf of inflorescence, often exceeding the total length of the latter; 4) fruits with a straight (as in S. emersum) as well as bent (as in S. gramineum) style. Interestingly, some populations of S. × longifolium are rich in terate forms that can be explained by back crossing with one of the parental species or pleiotropic mutation(s). It is established that S. × longifolium is not widespread in European Russia, is a typically freshwater species, occurring in the littoral zone of mesotrophic and dystrophic waterbodies (usually in lakes of glacier origin). At present, its appearance in lake ecosystems is due to accelerated eutrophication caused by increasing human activities. Perhaps earlier this hybrid formation occurred in peripheral zones of the range of S. gramineum under cyclic climate changes. Observations suggest that S. × longifolium exceeds S. gramineum in ecological potentials. At the same time, habitat features of the latter have an effect on the hybrid distribution potential (limitation of habitat spectrum) which is hardly exceed S. emersum by its ecological and coenotic characteristics.

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DNA barcoding reveals a new record of Potamogeton distinctus (Potamogetonaceae) and its natural hybrids, P. distinctus × P. nodosus and P. distinctus × P. wrightii (P. ×malainoides) from Myanmar

Cited by 7 publications

References 31 publications

Finding the pond through the weeds: eDNA reveals underestimated diversity of pondweeds

Finding the pond through the weeds: eDNA reveals underestimated diversity of pondweeds

Taxonomic treatment and phylogenetic analysis of the family Potamogetonaceae in Turkey

Morphology and ecological characteristics of Sparganium × longifolium (Typhaceae) in the Central part of European Russia

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