DNA barcoding reveals cryptic diversity of economic red algae, Pyropia (Bangiales, Rhodophyta): description of novel species from Korea

Koh, Young Ho; Kim, Myung Sook

doi:10.1007/s10811-018-1529-8

Cited by 21 publications

(10 citation statements)

References 33 publications

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“…The delimitation of species was undertaken recently using molecular tools, an advent that has redefined the boundaries of former taxa over the last 10 years (e.g., Broom et al 2002, Brodie et al 2007, Neefus et al 2008, Nelson 2013, Dumilag et al 2016, Guillemin et al 2016, Reddy et al 2018, Yang et al 2018. A high level of cryptic genetic diversity was found within Porphyra and Pyropia (Brodie et al 2007, Niwa et al 2009, Lindstrom et al 2015, Guillemin et al 2016, Koh and Kim 2018, Reddy et al 2018, Yang et al 2018. According to Zuccarello (2011), these new taxa that cannot be identified based on morphology could drive field ecology studies to misleading conclusions and impair our understanding of the evolutionary processes that generated this diversity.…”

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confidence: 99%

Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

Meynard

Zapata

Salas

et al. 2019

Journal of Phycology

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A recent molecular taxonomic study along the Chilean coast (18° S–53° S) described 18 candidate species of bladed Bangiales of which only two were formally described. Few studies focused on local genetic and morphological diversity of bladed Bangiales and attempted to determine their intertidal distribution in contrasting habitats, and none were performed in Chile. To delimit intertidal distributions of genetic species, 66 samples of bladed Bangiales were collected at Maitencillo (32° S) in four zones: a rocky platform, a rocky wall, and two boulders zones surrounded by sandy and rocky bottoms, respectively. These samples were identified based on sequences of the mitochondrial COI and chloroplast rbcL markers. We also collected 87 specimens for morphological characterization of the most common species, rapidly assessing their putative species identity using newly developed species‐diagnostic (PCR‐RFLP) markers. Eight microscopic and two macroscopic morphological traits were measured. We described and named three of four species that predominate in Maitencillo (including Pyropia orbicularis): Pyropia variabilis Zapata, Meynard, Ramírez, Contreras‐Porcia, sp. nov., Porphyra luchea Meynard, Ramírez, Contreras‐Porcia sp. nov., and Porphyra longissima Meynard, Ramírez, Contreras‐Porcia, sp. nov. With the exception of Po. longissima restricted to boulders surrounded by sandy bottom, and a morphotype of Py. variabilis restricted to rocky walls, the other species/morphotypes have overlapping intertidal distributions. Except for Po. longissima, which is clearly differentiated morphologically (longest and thinnest blades), we conclude that morphology is not sufficient to differentiate bladed Bangiales. Our findings underscore the importance of refining our knowledge of intrinsic and environmental determinants on the distribution of bladed Bangiales.

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confidence: 99%

Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

Meynard

Zapata

Salas

et al. 2019

Journal of Phycology

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“…The LSU analysis could not resolve Hypoglosseae species closely related to H. sabahense because there is less sequence data for this tribe (8 sequences) than rbcL (16 sequences). COI is known to be useful for DNA barcoding and detection of cryptic diversity (e.g., Koh and Kim 2018). However, the genetic distances of this gene (6.44-25.84%) were much larger than those of rbcL (2.37-17.69%) and LSU (1.74-21.20%), so the evolutionary rate of COI may be too high to unravel the relation-not resolve the relationship with H. sabahense (Supplementary Fig.…”

Section: Methodsmentioning

confidence: 99%

Morphological and molecular evidence for the recognition of <italic>Hypoglossum sabahense</italic> sp. nov. (Delesseriaceae, Rhodophyta) from Sabah, Malaysia

Wynne¹,

Kamiya²,

West³

et al. 2020

ALGAE

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Culture isolates of the genus Hypoglossum (Delesseriaceae, Rhodophyta) were obtained and their development and morphological structure over many years were followed in the laboratory. Molecular data (rbcL, large subunit ribosomal DNA, and cytochrome c oxidase subunit I) were obtained from these strains and evidence presented to recognize the new species: Hypoglossum sabahense from Sabah, Malaysia. Because various aspects of morphology in culture specimens differ significantly from types based on field specimens we have to rely mainly on the molecular criteria in ascribing a new taxonomic name here. This also is complicated by the major lack of molecular phylogenetic evidence for Hypoglossum and other Delesseriaceae. The 'Germling Emergence Method' and 'serendipity' are proving valuable in discovering significant new taxa from laboratory cultures which otherwise might never be known.

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“…Genera of macroalgae with well-known challenges in morphology-based identification include Ectocarpus (Leliaert andDe Clerck 2017, Montecinos et al 2017), Desmarestia (Yang et al 2014), Gracilaria (Kim et al 2010), Ulvella (Nielsen et al 2013), Fucus (Kucera 2010), Porphyra/Pyropia and relatives (Kucera 2010, Koh andKim 2018), Ulva ( Kucera 2010, Silva et al 2013, Grateloupia (Gavio and Fredericq 2002) and Cladophora (Hayakawa et al 2012, Taylor et al 2017) among others. For example, Ulva species have been underestimated in the Mediterranean, as a result of cryptic species within this group (Wolf et al 2012).…”

Section: Genus and Species Delimitation In Macroalgaementioning

confidence: 99%

The current state of DNA barcoding of macroalgae in the Mediterranean Sea: presently lacking but urgently required

Bartolo

Zammit

Peters³

et al. 2020

Botanica Marina

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AbstractThis review article explores the state of DNA barcoding of macroalgae in the Mediterranean Sea. Data from the Barcode of Life Data System (BOLD) were utilised in conjunction with a thorough bibliographic review. Our findings indicate that from around 1124 records of algae in the Mediterranean Sea, only 114 species have been barcoded. We thus conclude that there are insufficient macroalgal genetic data from the Mediterranean and that this area would greatly benefit from studies involving DNA barcoding. Such research would contribute to resolving numerous questions about macroalgal systematics in the area and address queries related to biogeography, especially those concerned with non-indigenous species. It could also possibly result in the development and application of better, cost-effective biodiversity monitoring programmes emanating from UN conventions and EU Directives. One possible way of achieving this is to construct DNA libraries via sequencing and barcoding, subsequently enabling better cost-effective biodiversity monitoring through environmental DNA metabarcoding.

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DNA barcoding reveals cryptic diversity of economic red algae, Pyropia (Bangiales, Rhodophyta): description of novel species from Korea

Cited by 21 publications

References 33 publications

Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

Morphological and molecular evidence for the recognition of <italic>Hypoglossum sabahense</italic> sp. nov. (Delesseriaceae, Rhodophyta) from Sabah, Malaysia

The current state of DNA barcoding of macroalgae in the Mediterranean Sea: presently lacking but urgently required

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