DNA barcoding reveals the temporal community composition of drifting fish eggs in the lower Hongshui River, China

Chen, Weitao; Zhu, Shuli; Yang, Jiping; Li, Xinhui; Li, Yuefei; Li, Jie

doi:10.1002/ece3.7943

Cited by 6 publications

(5 citation statements)

References 33 publications

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“…In addition, two field surveys in the middle and lower Pearl River between 2005 and 2018 ( Table S5 ) reported that cyprinids were the dominant taxon [ 38 , 39 ]. Furthermore, species diversity in the larval pool peaked in June, similar to larval pools in previous studies [ 24 , 37 ]. Consequently, to better protect fish resources in the middle and lower Pearl River, the month of June should be considered a conservation priority.…”

Section: Discussionsupporting

confidence: 85%

“…In total, 21 of the 28 identified species (75.0%) were cyprinids, suggesting that the cyprinids may be the dominant fish taxon in the middle Pearl River. The proportion of cyprinids identified here was similar to the proportions of cyprinids in larval pools from the lower Pearl River (73.0%; Table S5 ) [ 24 ] and the lower Hongshui River of the upper mainstem of the Pearl River (71.4%; Table S5 ) [ 37 ]. In addition, two field surveys in the middle and lower Pearl River between 2005 and 2018 ( Table S5 ) reported that cyprinids were the dominant taxon [ 38 , 39 ].…”

Section: Discussionsupporting

confidence: 62%

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Unraveling the Drifting Larval Fish Community in a Large Spawning Ground in the Middle Pearl River Using DNA Barcoding

Chen

et al. 2022

Animals

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Resolving the species composition of a larval pool in a spawning ground can provide novel insights into regional fish stocks and can support the development of effective monitoring and conservation policies. However, it is challenging to identify fish larvae to species due to their high diversity and dramatic phenotypic changes over development. In this study, we collected fish larvae in the Dongta spawning ground (Guiping City, Guangxi Province, China) in the middle reaches of the Pearl River between May and August 2018. We used a DNA barcoding approach to determine the species composition of the larval pool. A total of 905 larvae were chosen for molecular identification, of which 750 yielded high-quality barcoding sequences. Of these, 597 (≈79.6%), 151 (≈20.1%)/and 2 (≈0.3%) were assigned to 28 species, 8 genera, and 1 subfamily using the Barcode of Life Data System and GenBank nucleotide databases, respectively. Among the 28 identified species, 21 were cyprinids. Two species (Mugilogobius myxodermus and Pseudolaubuca engraulis) that were present only infrequently in previous adult surveys were abundant in the larval pool. Six invasive species were identified in the larval pool, implying that these species had successfully colonized the studied river section. Several migratory species common in the lower Pearl River were rare or absent in the investigated region, suggesting that dam construction in the Pearl River has had adverse effects on these migratory species. In summary, our study confirmed the applicability of DNA barcoding to studies of fish larval ecology and provided important reference data for fishery management and conservation in the Pearl River.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 62%

Unraveling the Drifting Larval Fish Community in a Large Spawning Ground in the Middle Pearl River Using DNA Barcoding

Chen

et al. 2022

Animals

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“…The high failure rate was most likely due to poor preservation at sea, the long time needed to pick out eggs preserved in low concentrations in ethanol, and the delayed DNA extraction process affected by the coronavirus epidemic in 2020. In previous studies, the sequencing success rate for fish eggs varied considerably, i.e., Ahern et al (2018) selected 6,422 eggs out of which 2,354 were sequenced successfully (COI/16S fragments, 36.66%); Liu et al (2018) selected 8,983 drifting eggs and obtained 7,933 cytb sequences (88.31%); and Chen et al (2021) selected 641 eggs and obtained 397 high-quality sequences (61.93%). It is considered that a low ethanol concentration and long storage in ethanol (>6 months) both negatively affect DNA stability and lead to DNA degradation (Zimmermann et al, 2008;Michaud and Foran, 2011;Marquina et al, 2021).…”

Section: Sequencing Success Rate Of Fish Eggsmentioning

confidence: 99%

“…In the last decade, molecular identification methods have been widely applied to identify fish eggs, including use of mitochondrial DNA fragments such as cytochrome c oxidase subunit I (COI) (Valdez-Moreno et al, 2010;Lewis et al, 2016;Leyva-Cruz et al, 2016;Ahern et al, 2018;Kerr et al, 2020;Chen et al, 2021), cytochrome b (cytb) (Liu et al, 2018), mtDNA PCR restriction fragment length polymorphism (RFLP) (Karaiskou et al, 2007;Stéphanie et al, 2010;Bui et al, 2011;Fitzcharles, 2012;Lelievre et al, 2012), and mtDNA control region (Dloop) (Shao et al, 2002). The DNA barcode method (COI) has been widely applied to define fish species, and facilitate the identification of fish eggs and larvae.…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Species Diversity Using Pelagic Fish Eggs in Spring and Late Autumn-Winter in the Eastern Beibu Gulf, China

et al. 2022

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The Beibu Gulf is considered as one of China’s four major fishing grounds, although the substantial overexploitation of fisheries has led to the collapse of many fish stocks, and to changes to spawning grounds in recent decades. Classifying fish eggs is an important way to monitor the recruitment process and identify the spawning sites of fish. However, the lack of a basis for morphological identification and difficulties in correctly identifying fish eggs based on morphological characteristics has limited scientific studies. In the present study, we identified fish eggs using molecular detection of cytochrome c oxidase subunit I and cytochrome b fragments. Ichthyoplankton surveys were conducted in the spring and late autumn–winter of 2020 in the eastern Beibu Gulf. Among the DNA extracted from the 873 chosen fish eggs, we successfully obtained 541 high-quality cytochrome c oxidase subunit I sequences and 41 high-quality cytochrome b sequences. We successfully identified 212 fish eggs (36.4%) from 32 species; 291 eggs (50.0%) showed ambiguous species delimitation, and 79 eggs (13.6%) could not be identified. Among the identified species, we found 25 species in spring and 25 species in late autumn–winter, out of which 18 species occurred in both seasons. We also obtained high resolution photographs of fish eggs at the species level for further morphological analysis and identification. The present study confirms the efficacy of using molecular methods to identify fish species from eggs and provides valuable information for protecting the spawning ground of economically valuable fish and for managing fishery resources.

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“…The Hongshui River is an important branch of the Xijiang from the Pearl River System, with abundant water energy resources and high fish diversity (He et al ., 2022; Tang et al ., 2021). After the Hongshui River cascade hydropower construction, studies on the biological basis of P. jordani seriously lack related field survey data (Chen et al ., 2021; Tang et al ., 2021). This study has focused on analysing the age structure, growth and reproduction characteristics of P. jordani and its resource status in the Hongshui River, and it aims to explore P. jordani after the cascade hydropower construction in the river on (a) the changes in its age structure and growth characteristics, (b) changes in reproductive characteristics and (c) current situation of fishery resources development.…”

Section: Introductionmentioning

confidence: 99%

Age, growth, reproduction and status of resource development of Ptychidio jordani, a critically endangered freshwater fish in the Hongshui River, China

Huang

et al. 2023

Journal of Fish Biology

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The age, growth, reproduction and resource development status of Ptychidio jordani, as a critically endangered freshwater fish in the Hongshui River, China, was studied in this work. A total of 525 specimens were collected monthly using the cages and gillnets from October 2021 to September 2022 in the Hongshui River. The scale was used for age determination, and the maximum age for both female and male was estimated to be 5 years and 3 years, respectively. Female and male P. jordani showed different growth patterns, which were expressed as Lt = 261.3 (1‐e−0.4885(t−0.1476)) and Lt = 251.2 (1‐e−0.4758(t+0.9643)), respectively. The overall sex ratio was 1:0.47 (female:male). Female attained sex maturity at 2.34 years (192 mm body length). Month variation of the gonad somatic index indicated that the spawning period occurred from April to October. The absolute fecundity was estimated at 9046 ± 3434 eggs per individual, and the relative fecundity was 38.08 ± 15.77 eggs per gram. The exploitation rate of female and male was 0.233 and 0.495, which indicated that P. jordani was not overfishing. This study provided data on the key life‐history traits of P. jordani, which has not been known previously and is essential for conservation strategy and policy development.

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DNA barcoding reveals the temporal community composition of drifting fish eggs in the lower Hongshui River, China

Abstract: Fish eggs and larvae represent important sources of information about the early phases of fish development, providing valuable data about spawning locations, spawning times, recruitment success rates, and the reproductive intensities of multiple species

Cited by 6 publications

References 33 publications

Unraveling the Drifting Larval Fish Community in a Large Spawning Ground in the Middle Pearl River Using DNA Barcoding

Unraveling the Drifting Larval Fish Community in a Large Spawning Ground in the Middle Pearl River Using DNA Barcoding

Molecular Identification of Species Diversity Using Pelagic Fish Eggs in Spring and Late Autumn-Winter in the Eastern Beibu Gulf, China

Age, growth, reproduction and status of resource development of Ptychidio jordani, a critically endangered freshwater fish in the Hongshui River, China

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