DNA base-stacking assay utilizing catalytic hairpin assembly-induced gold nanoparticle aggregation for colorimetric protein sensing

Chang, Chin‐Chen; Chen, Chie-Pein; Chen, Chen-Yu; Lin, Chii-Wann

doi:10.1039/c6cc01238h

Cited by 37 publications

(20 citation statements)

References 38 publications

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“…CHA based aptasensors have been successfully applied for proteins detection, including that of CEA, mucin‐1, prostate specific antigen (PSA), thrombin, and fibronectin. According to the initiating modes of CHA, CHA based protein aptasensors can be divided into the following three categories: target‐induced displacement of the initiator, target‐induced exposure of the initiator, and target‐induced joint binding‐initiating . Target‐induced displacement of the initiator means that an initiator complementary to the aptamer is released concomitantly with aptamer–protein binding.…”

Section: Cha Based Biosensors For Various Targetsmentioning

confidence: 99%

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Liu

Zhang

Xie

et al. 2019

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Nucleic acids are considered as perfect programmable materials for cascade signal amplification and not merely as genetic information carriers. Among them, catalytic hairpin assembly (CHA), an enzyme‐free, high‐efficiency, and isothermal amplification method, is a typical example. A typical CHA reaction is initiated by single‐stranded analytes, and substrate hairpins are successively opened, resulting in thermodynamically stable duplexes. CHA circuits, which were first proposed in 2008, present dozens of systems today. Through in‐depth research on mechanisms, the CHA circuits have been continuously enriched with diverse reaction systems and improved analytical performance. After a short time, the CHA reaction can realize exponential amplification under isothermal conditions. Under certain conditions, the CHA reaction can even achieve 600 000‐fold signal amplification. Owing to its promising versatility, CHA is able to be applied for analysis of various markers in vitro and in living cells. Also, CHA is integrated with nanomaterials and other molecular biotechnologies to produce diverse readouts. Herein, the varied CHA mechanisms, hairpin designs, and reaction conditions are introduced in detail. Additionally, biosensors based on CHA are presented. Finally, challenges and the outlook of CHA development are considered.

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Section: Cha Based Biosensors For Various Targetsmentioning

confidence: 99%

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Liu

Zhang

Xie

et al. 2019

Small

255

131

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“…Among nanomaterial-based colorimetric strategies, gold nanoparticles (AuNPs) are of great interest due to their unique physical and chemical properties [22]. AuNPs are known to be stabilized by electrostatic repulsion; aggregation-based AuNP colorimetric assays are widely used for the detection of protein [23][24][25], small molecule [26][27][28], and nucleic acid targets [29,30]. Moreover, such colorimetric assays are simply adaptable to a smartphone-based device without the use of costly instruments [31,32].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols

et al. 2020

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A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 μM), with a detection limit of 0.5 μM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.

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“…[21,22] Recently, catalytic hairpin assembly (CHA) has been widely used in sensitive analyses [23][24][25][26][27] but CHA-based strategies in living cells have been rarely reported, however. Additionally a CNNS-based CHA enzyme-free biosensor has not been used previously to detect intracellular miRNAs in cells.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the progress of oligonucleotide nanotechnology, oligonucleotide‐based sensing has achieved excellent signal amplification . Recently, catalytic hairpin assembly (CHA) has been widely used in sensitive analyses but CHA‐based strategies in living cells have been rarely reported, however. Additionally a CNNS‐based CHA enzyme‐free biosensor has not been used previously to detect intracellular miRNAs in cells.…”

Section: Introductionmentioning

confidence: 99%

In situ biosensor for detection miRNA in living cells based on carbon nitride nanosheets with catalytic hairpin assembly amplification

Liao

Pan

et al. 2017

Luminescence

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In this study, an ultrasensitive fluorescence turn-on assay for in situ sensing of intracellular microRNA (miRNA) was developed utilizing a carbon nitride nanosheet (CNNS) and a catalytic hairpin assembly (CHA). The CHA showed favourable signal amplification for low-level biomarkers, and CNNS was an excellent candidate as a fluorescence quencher and gene vector.Moreover, the hairpin DNA of CHA could be adsorbed onto the surface of CNNS. An enzymefree fluorescence biosensor for ultrasensitive sensing of intracellular miRNA in cells based on CHA and CNNS was designed. When faced with target miRNA, the fluorescence was recovered due to the miRNA, which could trigger cycling of CHA circuits, leading to the production of a marked enhanced fluorescence signal. Compared with traditional methods, the proposed method is convenient, with low cytotoxicity, and high specificity and ultrasensitivity. It has promising potential for detection low-level biomarkers. KEYWORDScarbon nitride nanosheet, catalytic hairpin assembly, intracellular signal amplification, microRNA, nanoprobe

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DNA base-stacking assay utilizing catalytic hairpin assembly-induced gold nanoparticle aggregation for colorimetric protein sensing

Cited by 37 publications

References 38 publications

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols

In situ biosensor for detection miRNA in living cells based on carbon nitride nanosheets with catalytic hairpin assembly amplification

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