DNA-Based Biosensor on Flexible Nylon Substrate by Dip-Pen Lithography for Topoisomerase Detection

Ferrara, Vittorio; Ottaviani, Alessio; Cavaleri, Felicia; Arrabito, Giuseppe; Ho, Yi‐Ping; Knudsen, Birgitta R.; Hede, Marianne Smedegaard; Pellerito, Claudia; Desideri, Alessandro; Feo, Salvatore; Marletta, Giovanni; Pignataro, Bruno

doi:10.1007/978-3-030-04324-7_39

Cited by 1 publication

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References 19 publications

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“…[ 16 ] To date, varied research has been done for making sensors based on DPN patterning methods, including gas sensors, [ 17 ] multiplexed biomimetic lipid membranes, [ 18 ] I–V measurements, [ 19 ] ribosome display, [ 20 ] and topoisomerases. [ 21 ] However, we are the first group utilizing the DPN method to fabricate nanosensors for heavy‐metal detection by DPN platforms. We developed a meta‐chemical surface (MCS) using DPN, which is somewhat analogous to the widely used meta‐surface, [ 14 ] by patterning a surface with a mixture of poly‐methyl methacrylate (PMMA) and nitrilotris (methylene) tri‐phosphonic acid (NTPH), which was used as the ink composition.…”

Section: Introductionmentioning

confidence: 99%

Development of Meta‐Chemical Surface by Dip‐Pen Nanolithography for Precise Electrochemical Lead Sensing

Yadav,

Shamir,

Kornweitz

et al. 2023

Small Methods

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Dip‐pen nanolithography (DPN) is a powerful and unique technique for precisely depositing tiny nano‐spherical cap shapes (nanoclusters) onto a desired surface. In this study, a meta‐chemical surface (MCS; a pattern with advanced features) is developed by DPN and applied to electrochemical lead sensing, yielding a calibration curve in the ppb range. An ink mixture of PMMA and NTPH (which binds to Pb (II), as supported by DFT calculations) is patterned over a Pt surface. The average height of the nanoclusters is ≈13 nm with a high surface area‐to‐volume ratio, which depends on the ink composition and the MCS surface. This ratio affected the sensitivity of the MCS as a detecting tool. The results indicate that the sensor's features can be controlled by the ability to control the size of the nanoclusters, attributed to the unique properties of the DPN production method. These results are significant for the water‐source purification industry.

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