DNA-based diagnostic tests for Salmonella strains targeting hilA, agfA, spvC and sef genes

Crăciunaş, C.; Keul, Anca-Livia; Flonta, Mirela; Cristea, Maria

doi:10.1016/j.jenvman.2010.07.027

Cited by 36 publications

(32 citation statements)

References 27 publications

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“…Salmonella-specific PCR with primers for the hilA gene denoted a clear abundance of this virulence gene; it was detected in 15 of 17 analyzed strains (88.24%), regardless of their serovars. Compared to previous reports, hilA was present in all of the Salmonella isolates from poultry [20,40].…”

Section: Discussioncontrasting

confidence: 90%

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Ammar

Mohamed

El-Hamid

et al. 2016

J Infect Dev Ctries

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Introduction: Salmonella serovars are one of the primary foodborne pathogens. Poultry consumption is responsible for the majority of disease cases worldwide. The prevalence of virulence determinants among Salmonella serovars appears to be lacking in Egypt. Therefore, this study investigated the occurrence, antibiotic resistance patterns, and virulence gene profiling of Salmonella serovars in broilers. Methodology: Three hundred samples from broiler chickens were examined for the presence of Salmonella by standard microbiological techniques. All Salmonella isolates were tested for their sensitivity against ten antibiotics and subjected to virulence genotyping by polymerase chain reaction (PCR). Results: The overall isolation percentage of Salmonella was 17%. Seven different serovars were found, with the main one being Salmonella Typhimurium (52.94%). Salmonella isolates were sensitive to most of the tested antibiotics, but they exhibited absolute resistance against amoxicillin/clavulanic acid. Nine Salmonella strains (52.94%) were resistant to at least three antibiotics. Further PCR investigations into 17 Salmonella strains revealed different distribution patterns of eight virulence determinants among the isolates. The invA gene was the most prevalent one (100%), followed by hilA (88.24%), stn (58.82%), and fliC genes (52.94%), while each of sopB and pefA genes had a similar prevalence (41.18%), and sefC and spvC genes had the lowest prevalence (11.76 and 5.88%, respectively). PCR genotyping allowed grouping of Salmonella strains into ten genetic profiles. Conclusions: These results will help in understanding the spread of virulence genotypes and antibiotic resistance among Salmonella serovars in broilers.

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Section: Discussioncontrasting

confidence: 90%

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Ammar

Mohamed

El-Hamid

et al. 2016

J Infect Dev Ctries

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“…to an increasing number of hosts because the acquisition and loss of fimbrial genes are involved in this process (Bäumler et al 1997). The high frequency of sefA is consistent with previous findings (Amini et al 2010, Craciunas et al 2012, and it can be considered a target gene to identify the serovar S. Enteritidis by PCR (Amini et al 2010).…”

Section: Discussionsupporting

confidence: 87%

“…All South Brazilian Salmonella Enteritidis isolates were positive for invA and hilA; similar observations have been reported by other studies around the world (Amini et al 2010, Campioni et al 2012, Craciunas et al 2012. It was expected that these genes would be detected in all of the isolates due to their importance for cell invasion.…”

Section: Discussionsupporting

confidence: 86%

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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RESUMO.-[Detecção de genes associados à virulência em cepas de Salmonella Enteritidis isoladas de frangos na região sul do Brasil.] Salmonella spp. estão entre os principais agentes causadores de doenças transmitidas por alimentos, e o sorovar Salmonella Enteritidis é o mais frequentemente isolado no mundo. A virulência de Salmonella spp. e a sua interação com o hospedeiro são processos complexos que envolvem fatores de virulência para sobreviver às defesas do hospedeiro. O objetivo deste estudo foi detectar genes de virulência em cepas de S. Enteritidis isoladas a partir de fontes avícolas no sul do Brasil. Ensaios de PCR foram desenvolvidos para a detecção de nove genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH are considered the main agents of foodborne disease and Salmonella Enteritidis is one of the most frequently isolated serovars worldwide. The virulence of Salmonella spp. and their interaction with the host are complex processes involving virulence factors to overcome host defenses. The purpose of this study was to detect virulence genes in S. Enteritidis isolates from poultry in the South of Brazil. PCR-based assays were developed in order to detect nine genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH and spvC) associated with the virulence in eighty-four isolates of S. Enteritidis isolated from poultry. The invA, hilA, sivH, sefA and avrA genes were present in 100% of the isolates; lpfA and sopE were present in 99%; agfA was present in 96%; and the spvC gene was present in 92%. It was possible to characterize the isolates with four different genetic profiles (P1, P2, P3 and P4), as it follows: P1, positive for all genes; P2, negative only for spvC; P3, negative for agfA; and P4, negative for lpfA, spvC and sopE. The most prevalent profile was P1, which was present in 88% of the isolates. Although all isolates belong to the same serovar, it was possible to observe variations in the presence of these virulence-associated genes between different isolates. The characterization of the mechanisms of virulence circulating in the population of Salmonella Enteritidis is important for a better understanding of its biology and pathogenicity. The frequency of these genes and the establishment of genetic profiles can be used to determine patterns of virulence. These patterns, associated with in vivo studies, may help develop tools to predict the ability of virulence of different strains.

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“…For confirmation, all environmental samples were screened using primers targeting hilA: 5′ -GTGAAATTAT-CGCCACGTTCGGGCAA-3′and 5′ -TCATCGCACCGT-CAAAGGAACC-3′ (Craciunas et al, 2012). Amplification was carried out in a 20 μL reaction mixture (10 μL 2× Power Taq PCR MasterMix [BioTeke Corporation, Beijing, China], 1 μL of 10 μM of each of the primers, 2 μL of DNA template, and 6 μL of ddH 2 O) in an Applied Biosystems Veriti 96-well thermal cycler (Life Technologies, CA, USA).…”

Section: Salmonella Detection Via Pcrmentioning

confidence: 99%

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

Liu

et al. 2018

J. Poult. Sci.

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Salmonella is the most common cause of foodborne illnesses worldwide. Poultry eggs are a major contamination source of Salmonella. The prevalence of Salmonella has been effectively reduced since a series of measures were taken to reduce contamination in egg-laying houses. In the present study, 1,512 environmental samples obtained from layer farms of different production scales were screened in a voluntary Salmonella survey study. Contaminations were detected using a PCR method. Genetic relationships among Salmonella samples were specified using molecular typing by enterobacterial repetitive intergenic consensus (ERIC)-PCR. The survey results showed that two layer farms, located in the Shandong and Hebei provinces, were contaminated with Salmonella. Thirty-one samples from these two farms, including feed, drinking nipples, egg collection belt, air inlets and outlets, air, overshoes, and eggshells, were identified as Salmonella-positive. It was observed that certain samples within the henhouses as well as in the egg collecting areas showed relatively high genetic similarities. The survey conclusively revealed minor Salmonella contamination in northern China. Moreover, various areas within the layer farms were identified as part of the propagation chain of Salmonella. Furthermore, evidence of cross-contamination of Salmonella was found in the laying houses and egg collection areas, even between these two regions. Therefore, it is necessary to establish routine Salmonella detection and subsequent environmental control measures in order to decrease the prevalence of Salmonella.

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DNA-based diagnostic tests for Salmonella strains targeting hilA, agfA, spvC and sef genes

Cited by 36 publications

References 27 publications

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

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