DNA-based genealogy reconstruction of Nebbiolo, Barbera and other ancient grapevine cultivars from northwestern Italy

Raimondi, Stefano; Tumino, Giorgio; Ruffa, Paola; Boccacci, Paolo; Gambino, Giorgio; Schneider, Anna

doi:10.1038/s41598-020-72799-6

Cited by 27 publications

(33 citation statements)

References 62 publications

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“…The mentioned founders, the historic “Fortana” and the SD related “Spergola” (alias “Vernaccia di Oristano”) are all quoted as early as XVII century on the Apennine foothills ( D’Onofrio et al, 2015 ; Bignami et al, 2015a ). This would support the Northern Apennines area as the likely homeland for all these grapes, including “Spergola”, later introduced to Sardinia as already asserted on the base of other genetic considerations ( Raimondi et al, 2020 ).…”

Section: Discussionmentioning

confidence: 68%

“…The software COLONY enables pedigree reconstruction using a likelihood-based method. The main limitation of this approach is related to its high computation demand that restricts its application to relatively small datasets (see, e.g., Crespan et al, 2020 ; Raimondi et al, 2020 ). The pedigree reconstruction method adopted in our study had the advantage of being applicable to a very large dataset, since it is not based on computation intensive likelihood estimations.…”

Section: Discussionmentioning

confidence: 99%

“…The large kin group of “Orsolina” merges varieties mainly from North and North Western Italy. Under the historic synonym of “Coccalona nera”, used in Piedmont, and “Rohrtraube blaurot”, used in lower Germany, this variety has been already identified as a genitor of many grapevines, together with one of its parent ( Raimondi et al, 2020 ). In the present study, several additional offspring of “Orsolina”-“Coccalona”-“Rohrtraube” were identified; most of them comes from Emilia Romagna and Tuscany, raising the offspring number of this prolific variety to as many as 25.…”

Section: Discussionmentioning

confidence: 99%

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Parentage Atlas of Italian Grapevine Varieties as Inferred From SNP Genotyping

D’Onofrio

Tumino²,

Gardiman

et al. 2021

Front. Plant Sci.

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The Italian grape germplasm is characterized by a high level of richness in terms of varieties number, with nearly 600 wine grape varieties listed in the Italian National Register of Grapevine Varieties and with a plethora of autochthonous grapes. In the present study an extended SNP genotyping has been carried out on Italian germplasm of cultivated Vitis vinifera subsp. sativa and Vitis hybrids. Several hundred Italian varieties maintained in the repositories of scientific Institutions and about one thousand additional varieties derived from previous studies on European, Southern Italy, Magna Graecia and Georgian germplasm were considered. The large genotyping data obtained were used to check the presence of homonyms and synonyms, determine parental relationships, and identify the main ancestors of traditional Italian cultivars and closely-related accessions. The parentage among a set of 1,232 unique varieties has been assessed. A total of 92 new parent-offspring (PO) pairs and 14 new PO trios were identified. The resulted parentage network suggested that the traditional Italian grapevine germplasm originates largely from a few central varieties geographically distributed into several areas of genetic influence: “Strinto porcino” and its offspring “Sangiovese”, “Mantonico bianco” and “Aglianico” mainly as founder varieties of South-Western Italy (IT-SW); Italian Adriatic Coast (IT-AC); and Central Italy with most varieties being offsprings of “Visparola”, “Garganega” and “Bombino bianco”; “Termarina (Sciaccarello)” “Orsolina” and “Uva Tosca” as the main varieties of North-Western Italy (IT-NW) and Central Italy. The pedigree reconstruction by full-sib and second-degree relationships highlighted the key role of some cultivars, and, in particular, the centrality of “Visparola” in the origin of Italian germplasm appeared clear. An hypothetical migration of this variety within the Italian Peninsula from South to North along the eastern side, as well as of “Sangiovese” from South to Central Italy along the Western side might be supposed. Moreover, it was also highlighted that, among the main founders of muscat varieties, “Moscato bianco” and “Zibibbo (Muscat of Alexandria)” have spread over the whole Italy, with a high contribution by the former to germplasm of the North-Western of the peninsula.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Parentage Atlas of Italian Grapevine Varieties as Inferred From SNP Genotyping

D’Onofrio

Tumino²,

Gardiman

et al. 2021

Front. Plant Sci.

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“…V. vinifera ‘Nebbiolo’ is one of the most important and ancient grape vines in the world [ 23 , 24 ]. Its area of cultivation is predominantly limited to northwestern Italy (hilly and mountainous areas of Piedmont, Aosta Valley, and Lombardy), although today it is also cultivated in California, Mexico, Australia, and other countries.…”

Section: Introductionmentioning

confidence: 99%

Secondary Metabolism and Defense Responses Are Differently Regulated in Two Grapevine Cultivars during Ripening

Gambino

Boccacci

Pagliarani

et al. 2021

IJMS

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Vitis vinifera ‘Nebbiolo’ is one of the most important wine grape cultivars used to produce prestigious high-quality wines known throughout the world, such as Barolo and Barbaresco. ‘Nebbiolo’ is a distinctive genotype characterized by medium/high vigor, long vegetative and ripening cycles, and limited berry skin color rich in 3′-hydroxylated anthocyanins. To investigate the molecular basis of these characteristics, ‘Nebbiolo’ berries collected at three different stages of ripening (berry pea size, véraison, and harvest) were compared with V. vinifera ‘Barbera’ berries, which are rich in 3′,5′-hydroxylated anthocyanins, using transcriptomic and analytical approaches. In two consecutive seasons, the two genotypes confirmed their characteristic anthocyanin profiles associated with a different modulation of their transcriptomes during ripening. Secondary metabolism and response to stress were the functional categories that most differentially changed between ‘Nebbiolo’ and ‘Barbera’. The profile rich in 3′-hydroxylated anthocyanins of ‘Nebbiolo’ was likely linked to a transcriptional downregulation of key genes of anthocyanin biosynthesis. In addition, at berry pea size, the defense metabolism was more active in ‘Nebbiolo’ than ‘Barbera’ in absence of biotic attacks. Accordingly, several pathogenesis-related proteins, WRKY transcription factors, and stilbene synthase genes were overexpressed in ‘Nebbiolo’, suggesting an interesting specific regulation of defense pathways in this genotype that deserves to be further explored.

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“…Significant efforts have been made to recover and identify local minor grapevine germplasm in many Italian regions, like Apulia, Campania, Emilia Romagna, Friuli Venezia Giulia, Liguria, Sardinia, Sicily, Tuscany (Costantini et al, 2005;De Mattia et al, 2007;Torello Marinoni et al, 2009;Carimi et al, 2010;Crespan et al, 2011;De Lorenzis et al, 2014;Schneider et al, 2014;Mercati et al, 2016;D'Onofrio et al, 2016;Gristina et al, 2017;Pastore et al, 2020), and variety pedigrees have been reconstructed for the most widespread and renowned ones and for those having local importance, helping to unravel the intricate skein of the structure of Italian grapevine populations (Di Vecchi-Staraz et al, 2007;Ruffa et al, 2016;Crespan et al, 2020;Raimondi et al, 2020;D'Onofrio et al, 2021). Similar efforts have been made in the Marche region, because the resulting knowledge is very useful in science and has cultural and marketing appeal.…”

Section: Introductionmentioning

confidence: 99%

Grapevine (<i>Vitis vinifera</i> L.) varietal assortment and evolution in the Marche region (central Italy)

Crespan¹,

Migliaro²,

Larger

et al. 2021

OENO One

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Italy is one of the richest countries in terms of grapevine varietal assortment, and the Marche region in central Italy has an ancient winegrowing tradition. Increasing interest in autochthonous grapevine varieties prompted efforts to recover and identify local minor germplasm also in this region, and to search for pedigree relationships and determine the evolution of varietal assortment. In the present study this was done using nuclear and chloroplast microsatellite markers and SNP markers from the Vitis18kSNP chip. Eighteen new genotypes were found, of which seventeen belonged to well-known, less known and even unknown vines found to be specific to the Marche region. The rearranged pedigree highlighted the complex intertwining relationships between Marche varieties. Second-degree relationships were also derived. Some minor Marche varieties have increased the number of parent-offspring related members of Garganega, Sangiovese, Crepolino/Visparola and Sciaccarello, which are varieties already recognised as founders or recurrent parents of many Italian cultivars. Crepolino/Visparola was shown to be a key variety in the evolution of the Marche varietal assortment, having played the role of parent and grandparent, as explained by the presence of this genotype in the region in ancient times. Surprisingly, Semidano, a well-known Sardinian variety, played a clear role as parent or grandparent of three minor Marche varieties, testifying to its presence in central Italy in ancient times. Incrocio Bruni 54 was confirmed as an offspring of Sauvignon and Verdicchio, as declared by the breeder. The parentage of Lacrima was completed: this variety was found to be a spontaneous cross between Nera Rada and Aleatico.A dendrogram of genetic similarity clearly resembled the groups defined with the pedigree reconstruction and it gave an indication of the genetic similarity of the varieties excluded from the strictest parentage links.

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DNA-based genealogy reconstruction of Nebbiolo, Barbera and other ancient grapevine cultivars from northwestern Italy

Cited by 27 publications

References 62 publications

Parentage Atlas of Italian Grapevine Varieties as Inferred From SNP Genotyping

Parentage Atlas of Italian Grapevine Varieties as Inferred From SNP Genotyping

Secondary Metabolism and Defense Responses Are Differently Regulated in Two Grapevine Cultivars during Ripening

Grapevine (<i>Vitis vinifera</i> L.) varietal assortment and evolution in the Marche region (central Italy)

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