DNA-based identification and descriptions of immatures of<i>Kempnyia</i>Klapálek (Insecta:Plecoptera) from Macaé River Basin, Rio de Janeiro State, Brazil

Avelino-Capistrano, Fernanda; Nessimian, Jorge Luiz; Santos-Mallet, Jacenir Reis dos; Takiya, Daniela Maeda

doi:10.1086/675226

Cited by 16 publications

(36 citation statements)

References 47 publications

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“…More recently, DNA barcoding (Hebert et al ., ) has been used to obtain species‐level adult–larva associations. This technique has been widely applied across a variety of insect groups, such as Coleoptera (Miller et al ., ; Ahrens et al ., ; Curiel & Morrone, ), Diptera (Trivinho‐Strixino et al ., ; Pramual & Wongpakam, ) and EPT taxa (Zhou et al ., ; Gattolliat & Monaghan ; Ruiter et al ., ; Avelino‐Capistrano et al ., ). Barcodes have also been used for associating anisomorphic developmental stages of other invertebrates (e.g.…”

Section: Introductionmentioning

confidence: 97%

Towards holomorphology in entomology: rapid and cost‐effective adult–larva matching using NGS barcodes

Yeo

Puniamoorthy²,

Ngiam

et al. 2018

Systematic Entomology

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In many taxa the morphology of females and immatures is poorly known because species descriptions and identification tools have a male bias. The root causes are problems with matching life-history stages and genders belonging to the same species. Such matching is time-consuming when conventional methods are used (e.g. rearing) and expensive when the stages are matched with DNA barcodes. Unfortunately, the lack of associations is not a trivial problem because it renders a large part of the phenome of insects unexplored, although larvae and females are useful sources of characters for descriptive and phylogenetic purposes. In addition, many collectors intentionally avoid females and immature stages, which skews survey results, interferes with collecting life-history information, and makes it less likely that rare species are discovered. These problems even exist for well-studied taxa like Odonata, where obtaining adult-larva matches relies largely on rearing. Here we demonstrate how the matching problem can be addressed with cost-effective tagged amplicon sequencing of a 313-bp segment of cox1 with next-generation sequencing (NGS) ('NGS barcoding'). We illustrate the value of this approach based on Singapore's odonate fauna which is of a similar size as the European fauna (Singapore, 122 extant species; Europe, 138 recorded species). We match the larvae and adults of 59 species by first creating a barcode database for 338 identified adult specimens representing 83 species. We then sequence 1178 larvae from a wide range of sources. We successfully barcode 1123 specimens, which leads to adult-larva matches for 59 species based on our own barcodes (55) and online barcode databases (4). With these additions, 84 of the 131 species recorded in Singapore have now been associated with a species name. Most common species are now matched (83%), and good progress has been made for vulnerable/near-threatened (55%), endangered (53%), and critically endangered species (38%). We used nondestructive DNA extraction methods in order to be able to use high-resolution imaging of matched larvae to establish a publicly available digital reference collection for odonates which is incorporated into 'Biodiversity of Singapore' (https://singapore.biodiversity.online/). We suggest that the methods described here are suitable for many insect taxa because NGS barcoding allows for fast and low-cost matching of well-studied life-history stages with neglected semaphoronts (eggs, larvae, females). We estimate that the specimen-specific amplicons in this study (c. 1500 specimens) can now be obtained within eight working days and that the laboratory and sequencing cost is c. US$600 (< US$0.40 per specimen).

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Section: Introductionmentioning

confidence: 97%

Towards holomorphology in entomology: rapid and cost‐effective adult–larva matching using NGS barcodes

Yeo

Puniamoorthy²,

Ngiam

et al. 2018

Systematic Entomology

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“…We note that evidence for somewhat higher levels of intraspecific divergence within some Plecoptera species (generally ca. 3-6%) seems to be accumulating in the literature (select taxa in: Zhou et al 2009, Sweeney et al 2011, Avelino-Capistrano et al 2014Mynott et al 2011, Elbrecht et al 2014. The nine Moselia specimens on the BOLD database have been grouped into four BINs based on similarity (genetic distance; Table 1) by BOLD's refined single linkage clustering algorithm (RESL).…”

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confidence: 99%

The Banded-wing Moselia infuscata (Claassen) Phenotype from California and Oregon, U.S.A. (Plecoptera: Leuctridae)

Gill

Kondratieff

Stark

et al. 2015

Zootaxa

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Moselia specimens from California and Oregon with a banded-wing phenotype were found to be indistinguishable morphologically from those of M. infuscata (Claassen) with typical wing pigment pattern. Preliminary DNA barcode data (Cytochrome c Oxidase subunit I [COI]), however, show significant genetic variation among four populations including three from northern California sites and one from southern Oregon. Although this genetic variation exceeded standard divergence thresholds often used to recognize distinct stream insect species, no new taxa are proposed at this time due to the preliminary nature of the data.

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“…However, despite the relevant growth in knowledge about Perlidae, in general, most studies have been restricted to the morphology of adults, mainly males. In fact, few nymphs have been associated with adults and described [ 21 ]. The nymphs of stoneflies live mainly in streams and are important indicators of environmental quality because, in general, they require well-oxygenated clean water to survive [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nymph rearing strategies are very time consuming and can hinder the nymph-adult association, mainly for rare species with narrow environmental tolerance, creating difficulties for a broad research program aimed at describing nymphs. In Brazil, many nymphs that have been described were associated with adults by rearing in the laboratory [ 16 , 23 , 26 , 35 ] or in streams in the field [ 16 , 22 , 25 , 26 , 33 , 36 , 37 ], and few species were associated using molecular tools [ 21 , 24 , 38 ]. Despite these efforts, currently, only 28 of the 145 species of Brazilian perlids have been associated and described.…”

Section: Introductionmentioning

confidence: 99%

Perlidae (Plecoptera) from the Paranapiacaba Mountains, Atlantic Forest, Brazil: Diversity and implications of the integrative approach and teneral specimens on taxonomy

Almeida

Bispo

2020

PLoS ONE

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The study of complementary sources of biological variation (e.g. morphological, molecular) has allowed a better understanding of biodiversity through the construction of an integrative taxonomy. Using this approach, specimens from the Paranapiacaba Mountains, southeastern Brazil, were studied to update the knowledge on the stonefly family Perlidae from the region, characterize the species, and make associations between nymphs and adults using a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene. The study also discusses the implications of integrative taxonomy and teneral specimens for the study of South American Perlidae. The molecular data were analyzed using Bayesian inference, Neighbor-joining, and delimiting species methods. Our results revealed that, in general, there was a morphological and molecular congruence between species. In the Paranapiacaba Mountains, three genera and 15 species were recorded: Anacroneuria boraceiensis Froehlich 2004, A. debilis (Pictet 1841) (new record), A. fiorentini De Ribeiro and Froehlich 2007 (new record), A. flintorum Froehlich 2002, A. iporanga Bispo and Froehlich 2004, A. itajaimirim Bispo and Froehlich 2004, A. polita (Burmeister 1913), A. subcostalis Klapálek 1921, A. tupi Bispo and Froehlich 2004 (with a description of the nymph), Kempnyia auberti Froehlich 1996, K. colossica (Navás 1934), K. flava Klapálek 1916, K. neotropica (Jacobson and Bianchi 1905) (including its new junior synonym K. petersorum Froehlich 1996), Kempnyia sp., and Macrogynoplax veneranda Froehlich 1984. COI sequences were obtained for 11 species, five of which had nymphs associated with adults. Among the five associated nymphs, the nymph of A. tupi is described here. The results of this study indicate that the color of adult teneral specimens differs from that of mature specimens. Given this, the synonym of K. neotropica and K. petersorum was proposed since these species have high morphological and molecular similarities and differ only in color patterns. In addition, the previous record of A. petersi Froehlich 2002 from the Paranapiacaba Mountains was invalidated since it was considered a teneral specimen of A. flintorum. These results suggest that the development of an integrative taxonomy is essential to continue advancing the study of Perlidae diversity in South America.

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DNA-based identification and descriptions of immatures ofKempnyiaKlapálek (Insecta:Plecoptera) from Macaé River Basin, Rio de Janeiro State, Brazil

Cited by 16 publications

References 47 publications

Towards holomorphology in entomology: rapid and cost‐effective adult–larva matching using NGS barcodes

Towards holomorphology in entomology: rapid and cost‐effective adult–larva matching using NGS barcodes

The Banded-wing Moselia infuscata (Claassen) Phenotype from California and Oregon, U.S.A. (Plecoptera: Leuctridae)

Perlidae (Plecoptera) from the Paranapiacaba Mountains, Atlantic Forest, Brazil: Diversity and implications of the integrative approach and teneral specimens on taxonomy

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