DNA binding and bending by dinuclear complexes comprising ruthenium polypyridyl centres linked by a bis(pyridylimine) ligand

McDonnell, Ursula; Hicks, Matthew R.; Hannon, Michael J.; Rodger, Alison

doi:10.1016/j.jinorgbio.2008.06.006

Cited by 60 publications

(18 citation statements)

References 48 publications

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“…The binding to double-strand DNA by the two metal centers of dinuclear complexes with rigid bridges are usually limited by their rigidity. Because the two metal centers are rigidly linked and consequently can not follow the curvature of the DNA, only one metal center may bind deeply to the DNA with the second metal center projecting out of the DNA [21][22][23][24][25][26][27][28][29][30][31][32][33][34]. The other kind of dinuclear Ru(II) complexes carrying a flexible chain should overcome the limitations and potentially allow both the metal centers to bind optimally to the DNA [35][36][37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

The interesting DNA-binding properties of three novel dinuclear Ru(II) complexes with varied lengths of flexible bridges

Zhang

Yuan

et al. 2011

Journal of Inorganic Biochemistry

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Section: Introductionmentioning

confidence: 99%

The interesting DNA-binding properties of three novel dinuclear Ru(II) complexes with varied lengths of flexible bridges

Zhang

Yuan

et al. 2011

Journal of Inorganic Biochemistry

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“…Such a quenched fluores- cence behavior of EB bound to DNA caused by the interaction between the copper(II) complexes and DNA is also found in other ternary copper complexes. 37,38) According to the classical Stern-Volmer Eq. 2 39) :…”

Section: Dna-binding Mode and Affinity Electronic Absorption Spectramentioning

confidence: 99%

Synthesis, Characterization, Cytotoxic Activities, and DNA-Binding Studies of Ternary Copper(II) Complexes with New Coumarin Derivatives

Jia

et al. 2010

CHEMICAL & PHARMACEUTICAL BULLETIN

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In this study, two novel complexes [Cu(MCVH)phen(H 2 O)] · ClO 4 (1) and [Cu(MCLH)phen(H 2 O)] · ClO 4 (2)(here, MCVH 2 ‫-7(؍‬hydroxy-4-methyl-8-coumarinyl) valine, MCLH 2 ‫-7(؍‬hydroxy-4-methyl-8-coumarinyl) leucine) have been synthesized and characterized by elemental analyses, molar conductance, infrared spectra (IR), 1 H-NMR and UV-Vis measurements. The interactions of them with calf thymus DNA (ct DNA) have been investigated by absorption spectroscopy, fluorescence spectroscopy, circular dichroism spectroscopy and viscosity measurements. Experimental results reveal an intercalative interaction with DNA for the complexes, furthermore the binding affinity of 2 is higher than that of 1 according to the calculated binding constant values. In addition, they were evaluated for their cytotoxic activities toward human prostate cancer cell (PC3), human liver cell (L02) and human myeloid leukemia cancer cell (HL-60) by acid phosphoatase assay. Both of them showed significant cytotoxic potency.Key words copper(II) complex; coumarin derivative; DNA-binding; intercalative mode; cytotoxic activity Chem. Pharm. Bull. 58(8) 1003-1008 (2010) © 2010 Pharmaceutical Society of Japan * To whom correspondence should be addressed. e-mail: tangn@lzu.edu.cn; wangqin329@yahoo.com.cn concentrations of 1 and 2 as constant at 10 mM while varying the concentration of ct DNA. Fitting was completed using an Origin 6.0 spreadsheet, where values of the binding constants K b were calculated.Fluorescence spectra of the competitive binding experiments were carried out by maintaining the EB and ct DNA concentration at 3 mM and 30 mM, respectively, while increasing the concentrations of the complexes. Fitting was completed using an Origin 6.0 spreadsheet, where values of K q were calculated.Viscosity experiments were carried out on an Ubbelodhe viscometer, immersed in a thermostated water-bath maintained at 25.0Ϯ0.1°C. Titrations were performed for the complexes (1-5 mM), and each compound was introduced into DNA solution (50 mM) present in the viscometer. Flow time was measured with a digital stopwatch and each sample was measured three times and an average flow time was calculated.The CD spectra of DNA were recorded on a Jasco J-810 spectropolarimeter at 25.0Ϯ0.1°C. Calf thymus DNA used were 120 mM in concentration and compounds solutions was added to a ratio of 1 : 1 (DNA/compound). Each sample solution was scanned in the range of 200-350 nm. CD spectrum was generated which represented the average of three scans from which the buffer background had been subtracted.Acid Phosphoatase Assay The reagent, p-nitrophenyl phosphate (p-NPP), was obtained from Amresco. The compounds synthesized were dissolved in dimethyl sulphoxide (DMSO) and diluted in culture medium. The final concentration of DMSO in cultures was always not exceeding 0.5% (v/v), which did not cause toxicity. The PC3 and HL-60 cells obtained from ATCC were maintained in Dulbecco's modified Eagle's medium (DMEM) medium with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 10...

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“…The flexible chain-linked dinuclear Ru(II) complexes could potentially allow both the metal centres to bind optimally to the DNA (see Scheme 1 for representative examples of alkyl chainlinked dinuclear Ru(II) complexes). In contrast, the rigid bridges have the limitation that cannot follow the curvature of the DNA, only one metal center may bind deeply to the DNA with the second metal center projecting out of the DNA [17][18][19]. In recent decades, Hip-based Ru(II) complexes have been well developed as an attractive family of DNA binders [10][11][12][13]18,20], but the alkyl chainlinked Hip-based dinuclear Ru(II) complexes still remain poorly explored.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, DNA binding and photocleavage, and cellular uptake of an alkyl chain-linked dinuclear ruthenium(II) complex

Liu

Jin

Zhang

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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DNA binding and bending by dinuclear complexes comprising ruthenium polypyridyl centres linked by a bis(pyridylimine) ligand

Cited by 60 publications

References 48 publications

The interesting DNA-binding properties of three novel dinuclear Ru(II) complexes with varied lengths of flexible bridges

The interesting DNA-binding properties of three novel dinuclear Ru(II) complexes with varied lengths of flexible bridges

Synthesis, Characterization, Cytotoxic Activities, and DNA-Binding Studies of Ternary Copper(II) Complexes with New Coumarin Derivatives

Synthesis, DNA binding and photocleavage, and cellular uptake of an alkyl chain-linked dinuclear ruthenium(II) complex

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