DNA binding and dispersion activities of titanium dioxide nanoparticles with UV/vis spectrophotometry, fluorescence spectroscopy and physicochemical analysis at physiological temperature

Patel, Suhani; Patel, Palak; Undre, Sachin B.; Pandya, Shivani R.; Singh, Man; Bakshi,

doi:10.1016/j.molliq.2015.11.002

Cited by 51 publications

(28 citation statements)

References 27 publications

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“…To the best of our knowledge, we report here the first study including both, Chromosome aberration and Comet assays at low dose exposure for 24 h. Keeping in mind the widespread use of TiO 2 NPs and insufficient information regarding its genotoxicity, we have also attempted mechanistic analysis by studying its binding affinity with DNA using fluorescence titration method. The extent of dispersion and stability of TiO 2 NPs in water and RPMI-1640 cell culture media was assessed by Dynamic Light Scattering and Zeta Potential measurements; the results of which supported our previous study where dispersion of NPs was found to be better in media than water (Patel et al 2016). Since, research in the field of in vitro TiO 2 NPs toxicity with proper characterization and effect of low dose exposure in terms of genotoxicity and carcinogenicity is important, we report the DNA damaging potential of TiO 2 NPs in their 'nano' mono-dispersed form in human lymphocyte cultures at 25, 75 and 125 lM when exposed for 24 h by Chromosome aberration assay and Comet assay.…”

Section: Introductionsupporting

confidence: 84%

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Titanium dioxide nanoparticles: an in vitro study of DNA binding, chromosome aberration assay, and comet assay

Patel

Bakshi

2017

Cytotechnology

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Engineered titanium dioxide nanoparticles (TiO NPs) are extensively used in cosmetic, pharmaceutical and other industries globally due to their unique properties, which has raised concern for biosafety. Genotoxicity assessment is an important part of biosafety evaluation; we report in vitro cytogenetic assays for NPs considering their unique physicochemical characteristics to fill the gap of laboratory data regarding biological safety along with mechanistic study for mode of interaction of NP with genetic material. Comet and chromosome aberration assay (CA assay) using short-term human peripheral blood cultures following exposure to TiO NPs; along with physicochemical parameters for stability of nano form in cultures; and DNA binding activity were carried out. The dynamic light scattering and zeta potential measurements revealed mono dispersion in media. The fluorescence spectroscopy for binding affinity of TiO NPs and human genomic DNA showed binding constant (K), 4.158 × 10 M indicating strong binding affinity and negative ΔG value suggesting spontaneous DNA binding supporting its genotoxic potential. Following in vitro exposure to TiO NPs for 24 h, the cultures were analyzed for comet and CA assays, which showed significant results (p < 0.05) for % DNA intensity in tail, Olive Tail Moment and frequency of Chromosomal aberrations (CA) at 75 and 125 μM but not at 25 μM.

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Section: Introductionsupporting

confidence: 84%

“…5 was 4.158 9 10 6 M -1 indicating its strong binding capacity with DNA. Two studies have previously shown a direct chemical interaction between TiO 2 NPs and DNA through the DNA phosphate group (Patel et al 2016;Zhu et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Titanium dioxide nanoparticles: an in vitro study of DNA binding, chromosome aberration assay, and comet assay

Patel

Bakshi

2017

Cytotechnology

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“…Figure 11 illustrates the structure of DNA. The base pairs of DNA possess strong absorbance at 260 nm [45] ; if the DNA helix is denatured, base stacking is destroyed and UV absorbance goes up. The effect of PMS and most NP/PMS samples was to increase absorbance at 260nm above that for the DNA introduced (both in light and dark conditions and in the supernatant and PMS sediment).…”

Section: Doublehelical 220mentioning

confidence: 99%

“…For example, H-bonds, hydrophobic effect and stacking interactions between complementary bases hold the two strands of DNA together. When NPs/PMS were added, the base-base interaction was reduced in the DNA structure, owing to many bases becoming free form and H-bond breakage leading to an increase absorbance [45] . Furthermore, the presence of a single stranded DNA will be higher than double stranded DNA in the same concentration.…”

Section: Doublehelical 220mentioning

confidence: 99%

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Nanoengineering the antibacterial activity of biosynthesized nanoparticles of TiO₂, Ag, and Au and their nanohybrids with Portobello mushroom spore (PMS) (TiO_x/PMS, Ag/PMS and Au/PMS) and making them optically self-indicating

et al. 2016

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Results show that nanoparticles (NPs) can be biosynthesized at room temperature on the reductive and chelating surfaces of Portobello mushroom spores (PMS). Using this green approach TiOx, Ag, Au, Ag-TiOx and Au-TiOx NPs have been prepared. These were characterized by TEM, SIMS and FTIR-FTIR. TiOx/PMS, Ag-TiOx/PMS, Au-TiOx/PMS and Ag/PMS were active in bacterial inhibition towards Eschericia coli and Staphylococcus aureus, but Au/PMS was not active (suggesting a strong Au-PMS interaction). TiOx/PMS, Ag/PMS and Ag-TiOx/PMS were equally active in an antibacterial and an antifungal sense when tested against Asperillus and Candide. All samples (except Ag-TiOx/PMS and Au-TiOx/PMS) showed an interesting interaction with DNA. We report on the process of fine-tuning these antibacterial properties, progress on making these nanomaterials optically selfindicating and movement towards optical control of their antibacterial activity. Au-TiOx/PMS shows a surface plasmon resonance (SPR) with a maximum at 518 nm that might be useful in following its anti-bacterial properties (i.e. making the bionanomaterial self-indicating). The future of such green bio-nanomaterials is strong.

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Enhancing Infected Wound Healing Through Scavenging Reactive Oxygen Species Using Synergetic Composites of Sub‐Nanoscale TiO₂ with DNA

Xu,

Fan,

et al. 2024

Adv Funct Materials

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Oxidative stress (OS) resulting from excessive reactive oxygen species (ROS) is the initial pathogenesis of many diseases, thus various pharmaceutical materials are explored to scavenge ROS. However, the medical applications of most ROS‐scavenging materials are limited due to side effects and low bio‐stability. DNA has emerged as a promising ROS‐scavenging material with excellent biosafety and programmability, but the efficiency needs to be improved by developing new fabrication methods. Here, a sub‐nanoscale TiO2 composite modified with DNA with excellent biostability, biocompatibility, and enhanced ROS‐scavenging efficiency for medical applications is presented. The sub‐nanoscale TiO2‐DNA (SNTD) composite exhibits higher scavenging capacities for multiple ROS including ·OH, H2O2, and O2•−. Additionally, it can regulate macrophages from pro‐inflammatory to anti‐inflammatory phenotype. In vivo experiments show that the nanocomposites reduce ROS concentration, decrease inflammatory cell infiltration, accelerate re‐epithelization, and promote collagen regeneration, thereby enhancing the healing of infected skin wounds.

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DNA binding and dispersion activities of titanium dioxide nanoparticles with UV/vis spectrophotometry, fluorescence spectroscopy and physicochemical analysis at physiological temperature

Cited by 51 publications

References 27 publications

Titanium dioxide nanoparticles: an in vitro study of DNA binding, chromosome aberration assay, and comet assay

Titanium dioxide nanoparticles: an in vitro study of DNA binding, chromosome aberration assay, and comet assay

Nanoengineering the antibacterial activity of biosynthesized nanoparticles of TiO₂, Ag, and Au and their nanohybrids with Portobello mushroom spore (PMS) (TiO_x/PMS, Ag/PMS and Au/PMS) and making them optically self-indicating

Enhancing Infected Wound Healing Through Scavenging Reactive Oxygen Species Using Synergetic Composites of Sub‐Nanoscale TiO₂ with DNA

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DNA binding and dispersion activities of titanium dioxide nanoparticles with UV/vis spectrophotometry, fluorescence spectroscopy and physicochemical analysis at physiological temperature

Cited by 51 publications

References 27 publications

Titanium dioxide nanoparticles: an in vitro study of DNA binding, chromosome aberration assay, and comet assay

Titanium dioxide nanoparticles: an in vitro study of DNA binding, chromosome aberration assay, and comet assay

Nanoengineering the antibacterial activity of biosynthesized nanoparticles of TiO2, Ag, and Au and their nanohybrids with Portobello mushroom spore (PMS) (TiOx/PMS, Ag/PMS and Au/PMS) and making them optically self-indicating

Enhancing Infected Wound Healing Through Scavenging Reactive Oxygen Species Using Synergetic Composites of Sub‐Nanoscale TiO2 with DNA

Contact Info

Product

Resources

About

Nanoengineering the antibacterial activity of biosynthesized nanoparticles of TiO₂, Ag, and Au and their nanohybrids with Portobello mushroom spore (PMS) (TiO_x/PMS, Ag/PMS and Au/PMS) and making them optically self-indicating

Enhancing Infected Wound Healing Through Scavenging Reactive Oxygen Species Using Synergetic Composites of Sub‐Nanoscale TiO₂ with DNA