DNA binding, cleavage, apoptosis and cytotoxicity studies of three heteroleptic nickel complexes bearing β-diketones

Sousa, Luana M.; Souza, Wesley Almeida; Paixão, Drielly A.; Fazzi, Rodrigo Boni; Tezuka, Daiane Y.; Lopes, Carla D.; Carneiro, Zumira Aparecida; Moreira, Mariete Barbosa; Pivatto, Marcos; Netto, Adelino V.G.; Albuquerque, Sérgio de; Ferreira, Francis Barbosa; Oliveira, Ronaldo Junio de; Resende, Jackson Antônio Lamounier Camargos; Lino, Ricardo Campos; Júnior, Robson José de Oliveira; Ferreira, Ana Maria da Costa; Guerra, Wendell

doi:10.1016/j.ica.2020.119824

Cited by 25 publications

(4 citation statements)

References 49 publications

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“…Structures assembled in Cu 2+ and Ni + showed a smear, indicating potential DNA cleavage reported earlier in these ions. [44,45] We then used AFM to visually examine the formation of the DNA origami triangle in the conditions that showed proper assembly on agarose gels. AFM analysis confirmed proper assembly of the DNA origami triangle in 10 mm Ca 2+ and Ba 2+ ions compared to the control structures assembled in 10 mm Mg 2+ (Figure 3d).…”

Section: Assembly Of a Dna Origami Trianglementioning

confidence: 99%

Self‐Assembly of DNA Nanostructures in Different Cations

Rodriguez

Gandavadi

Mathivanan

et al. 2023

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The programmable nature of DNA allows the construction of custom‐designed static and dynamic nanostructures, and assembly conditions typically require high concentrations of magnesium ions that restricts their applications. In other solution conditions tested for DNA nanostructure assembly, only a limited set of divalent and monovalent ions are used so far (typically Mg2+ and Na+). Here, we investigate the assembly of DNA nanostructures in a wide variety of ions using nanostructures of different sizes: a double‐crossover motif (76 bp), a three‐point‐star motif (~134 bp), a DNA tetrahedron (534 bp) and a DNA origami triangle (7221 bp). We show successful assembly of a majority of these structures in Ca2+, Ba2+, Na+, K+ and Li+ and provide quantified assembly yields using gel electrophoresis and visual confirmation of a DNA origami triangle using atomic force microscopy. We further show that structures assembled in monovalent ions (Na+, K+ and Li+) exhibit up to a 10‐fold higher nuclease resistance compared to those assembled in divalent ions (Mg2+, Ca2+ and Ba2+). Our work presents new assembly conditions for a wide range of DNA nanostructures with enhanced biostability.

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Section: Assembly Of a Dna Origami Trianglementioning

confidence: 99%

Self‐Assembly of DNA Nanostructures in Different Cations

Rodriguez

Gandavadi

Mathivanan

et al. 2023

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“…In this respect and since nickel lies in the same group as platinum, fundamental strategies to design pharmaceuticals based on Ni( ii ) complexes have become a pivotal area of research. 9–12 Ni( ii ) complexes are regarded as one of the most promising alternatives to the traditional cisplatin as an anticancer drug, an idea supported by a considerable number of research articles describing the synthesis, DNA binding, and cytotoxic activities of these complexes. 13 Earlier experimental studies with calf thymus DNA have shown that Ni complexes have a very high potential to be used as DNA inhibitors.…”

Section: Introductionmentioning

confidence: 99%

Ni(ii)-polypyridyl complexes as potential DNA binders

et al. 2023

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“…Among non-platinum based compounds, in 1995, Hanauske and coworkers introduced budotitane as the first titanium(IV) drug for cancer treatment [10]. Other cytotoxic β-dicarbonyls exploit the presence of Ru II [11], Co II [12], Cu II [13,14], Ni II [15,16] and oxovanadium(IV) [17].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization and DNA-Binding Affinity of a New Zinc(II) Bis(5-methoxy-indol-3-yl)propane-1,3-dione Complex

et al. 2021

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The novel zinc(II) µ-oxo-bridged-dimeric complex [Zn2(µ-O)2(BMIP)2] (BMIP = 1,3-bis(5-methoxy-1-methyl-1H-indol-3-yl)propane-1,3-dione), 1, was synthetized and fully characterized. The spectral data indicate a zincoxane molecular structure, with the BMIP ligand coordinating in its neutral form via its oxygen atoms. Structural changes in 1 in dimethylsulfoxide (DMSO) were evidenced by means of spectroscopic techniques including infrared absorption and nuclear magnetic resonance, showing DMSO entrance in the coordination sphere of the metal ion. The resulting complex [Zn2(µ-O)2(BMIP)2(DMSO)], 2, readily reacts in the presence of N-methyl-imidazole (NMI), a liquid-phase nucleoside mimic, to form [Zn2(µ-O)2(BMIP)2(NMI)], 3, through DMSO displacement. The three complexes show high thermal stability, demonstrating that 1 has high affinity for hard nucleophiles. Finally, with the aim of probing the suitability of this system as model scaffold for new potential anticancer metallodrugs, the interactions of 1 with calf thymus DNA were investigated in vitro in pseudo-physiological environment through UV-Vis absorption and fluorescence emission spectroscopy, as well as time-resolved fluorescence studies. The latter analyses revealed that [Zn2(µ-O)2(BMIP)2(DMSO)] binds to DNA with high affinity upon DMSO displacement, opening new perspectives for the development of optimized drug substances.

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DNA binding, cleavage, apoptosis and cytotoxicity studies of three heteroleptic nickel complexes bearing β-diketones

Cited by 25 publications

References 49 publications

Self‐Assembly of DNA Nanostructures in Different Cations

Self‐Assembly of DNA Nanostructures in Different Cations

Ni(ii)-polypyridyl complexes as potential DNA binders

Synthesis, Characterization and DNA-Binding Affinity of a New Zinc(II) Bis(5-methoxy-indol-3-yl)propane-1,3-dione Complex

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