1997
DOI: 10.1074/jbc.272.18.12083
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DNA Binding Is Not Sufficient for H-NS-mediated Repression ofproU Expression

Abstract: H-NS is a major component of bacterial chromatin and influences the expression of many genes. H-NS has beenshown to exhibit a binding preference for certain ATrich curved DNA elements in vitro. In this study we have addressed the factors that determine the specificity of H-NS action in vitro and in vivo. In bandshift studies, H-NS showed a slight binding preference for all curved sequences tested whether GC-based or AT-based; the specific architecture of the curve also influenced H-NS binding. In filter retent… Show more

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Cited by 61 publications
(56 citation statements)
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“…Mobility shift assays with agarose gels were performed as described previously (16), except that the reaction volume was 20 l and the preparations were incubated for 15 min at 37°C. A 100-bp DNA ladder (MBI Fermentas) was used as the competitor DNA.…”
Section: Methodsmentioning
confidence: 99%
“…Mobility shift assays with agarose gels were performed as described previously (16), except that the reaction volume was 20 l and the preparations were incubated for 15 min at 37°C. A 100-bp DNA ladder (MBI Fermentas) was used as the competitor DNA.…”
Section: Methodsmentioning
confidence: 99%
“…On the other hand, there is another possibility to explain the deficiency of homologous recombination in the hns single or hns stpA double mutant. It is known that H-NS binds preferentially to highly curved DNA (Jordi et al, 1997) and can even bend non-curved DNA through its binding. Oligomerization domain of this protein seems to play a critical role in the bending (Spurio et al, 1997).…”
mentioning
confidence: 99%
“…It has been shown in vitro that indeed H-NS binds with a higher affinity to strongly curved DNA when compared with noncurved or moderately curved DNA, independent of the base composition (29,30). Footprints of H-NS on the most strongly curved DNA fragment examined show specific protection of the curved region (31).…”
mentioning
confidence: 99%