DNA-binding of zinc(II) and nickel(II) salphen-like complexes extrapolated at 1 M salt concentration: Removing the ionic strength bias in physiological conditions

Characterization of the Binding Properties of Sorafenib to c-MYC G-Quadruplexes: Evidence for Screening Potential Ligands

“…UV spectra of Sor (10 μM) were recorded in the presence or absence of different concentrations of G4 (0, 1.5, 3.0, 4.5, 6.0, 7.8, 9.3, 11.1 μM), and the corresponding absorption spectra of G4 were subtracted to take into account of the DNA contribution as a correction of the signal of the Sor–G4 complexes. The binding constant formula is calculated by the following formula , false[ DNA false] / Δ ε ap = [ DNA ] / normalΔ ε + 1 / [ false( normalΔ ε false) × K a ] Among those, Δε ap = |ε a – ε f |. Δε = |ε b – ε f |; ε a is the apparent molar extinction coefficient of Sor, which is obtained from the ratio of the absorbance of Sor in the presence of different concentrations of G4 to the concentrations of Sor; ε b is the molar extinction coefficient at 268 nm of Sor fully bound to DNA; and ε f is the molar extinction coefficient of the free Sor.…”

“…UV spectra of Sor (10 μM) were recorded in the presence or absence of different concentrations of G4 (0, 1.5, 3.0, 4.5, 6.0, 7.8, 9.3, 11.1 μM), and the corresponding absorption spectra of G4 were subtracted to take into account of the DNA contribution as a correction of the signal of the Sor−G4 complexes. The binding constant formula is calculated by the following formula 17,18…”

How the Metal Ion Affects the 1H NMR Chemical Shift Values of Schiff Base Metal Complexes: Rationalization by DFT Calculations

“…In the past few years, we have been involved in the synthesis and characterization of square planar Schiff base transition-metal complexes, also investigating their DNA-binding properties. − In this context, we have recently reported on different metal complexes of a substituted Salphen ligand, H 2 L1 in Figure . In these complexes, the metal ion is always charged 2+ and copper­(II) is also included.…”

G-quadruplex DNA binding properties of novel nickel Schiff base complexes with four pendant groups

“…5,23,24 It should also be noted, however, that there have been reports of complexes not containing structural features typically associated with favourable G-quadruplex binding, that are nonetheless able to effectively interact with these nucleic acid structures. 25,26 This includes a study involving a six-coordinate cobalt complex with two trans ammine ligands in its coordination sphere. 27 This complex showed little evidence of interacting with double stranded DNA (dsDNA).…”