DNA binding properties of the purified Antennapedia homeodomain.

Affolter, Markus; Percival‐Smith, Anthony; Müller, Martin; Leupin, Werner; Gehring, Walter

doi:10.1073/pnas.87.11.4093

Cited by 180 publications

(114 citation statements)

References 36 publications

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“…The differences in binding between the specific and non-specific interactions were mainly due to differences in dissociation rates. KD values for the complexes between the Antp homeodomain and the A element have been obtained by equilibrium studies and by kinetic binding studies using the gel shift method, and they have been reported to be 1.6X10 -9 M and 1.8 x 10 -1° M, respectively [10]. These values are even smaller than those obtained in the present study.…”

Section: Association and Dissociation Rates For Specific Binding Of Tcontrasting

confidence: 64%

“…Each homeodomain that is encoded by a homeobox can bind specifically to a region of double-stranded (ds) DNA with a specific recognition sequence [2][3][4][5][6]. The interactions between homeodomains and DNA have been extensively analyzed by foot-printing methods and gel shift assays [6][7][8][9][10][11][12][13][14]. Direct structural analysis of the complexes has also been performed with NMR and X-ray [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Kinetics of binding of Antp homeodomain to DNA analyzed by measurements of surface plasmon resonance

Seimiya¹,

Kurosawa²

1996

FEBS Letters

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The kinetics of binding of the Antp homeodomain to three kinds of DNA fragment were analyzed by measurements of surface plasmon resonance at various temperatures. Non-specific and specific binding of the homeodomain to DNA was examined. In the case of non-specific binding, the association rate constant (kass) was estimated to be 1.41-2.62X 105 M -1 s -1 and the dissociation rate constant (kdiss) was 1.36-3.10 X 10 -2 s -1, thus, the dissociation constant (KI)) was 0.847-1.72 × 10 7 M. The association seemed to be driven by entropy. In the case of specific binding, by contrast, the enthalpy term seemed to contribute more to the binding than did the entropy term. The kass was 2.04-2.59X105 M -1 s -1 and the kdiss was 0.759-1.16X10 -3 s -1, thus, the /to was 2.93-5.69X 10 -9 M. These values were measured under the condition of 150 mM NaCl. Both interactions were strongly dependent on the concentration of NaC1. The /to at 50 mM NaCI became several tens of times smaller than those at 150 mM. Possible reasons for the differences between non-specific and specific interactions are discussed.

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Section: Association and Dissociation Rates For Specific Binding Of Tcontrasting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Kinetics of binding of Antp homeodomain to DNA analyzed by measurements of surface plasmon resonance

Seimiya¹,

Kurosawa²

1996

FEBS Letters

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“…A candidate for the activator protein is CEH-36, a homeodomain protein that is expressed symmetrically in AWC neurons and required for AWC fates (Lanjuin et al 2003;Koga and Ohshima 2004). ceh-36 mutants fail to express both str-2 or srsx-3 in AWC, and CEH-36 and other Otx-class K50 homeodomains bind the sequence TAATCC, which is similar to the AATCCC sequence that activates AWC expression of the srsx-3 promoter (Affolter et al 1990;Schier and Gehring 1993;Lanjuin et al 2003;Chaney et al 2005;Berger et al 2008;Etchberger et al 2009). NSY-7 might compete with CEH-36 or another activator for binding to the compound 11-bp element, specifically repressing expression in AWC ON .…”

Section: Discussionmentioning

confidence: 99%

Transcriptional regulation and stabilization of left–right neuronal identity in C. elegans

Lesch

Gehrke²,

Bulyk³

et al. 2009

Genes Dev.

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At discrete points in development, transient signals are transformed into long-lasting cell fates. For example, the asymmetric identities of two Caenorhabditis elegans olfactory neurons called AWC ON and AWC OFF are specified by an embryonic signaling pathway, but maintained throughout the life of an animal. Here we show that the DNAbinding protein NSY-7 acts to convert a transient, partially differentiated state into a stable AWC ON identity. Expression of an AWC ON marker is initiated in nsy-7 loss-of-function mutants, but subsequently lost, so that most adult animals have two AWC OFF neurons and no AWC ON neurons. nsy-7 encodes a protein with distant similarity to a homeodomain. It is expressed in AWC ON , and is an early transcriptional target of the embryonic signaling pathway that specifies AWC ON and AWC OFF ; its expression anticipates future AWC asymmetry. The NSY-7 protein binds a specific optimal DNA sequence that was identified through a complete biochemical survey of 8-mer DNA sequences. This sequence is present in the promoter of an AWC OFF marker and essential for its asymmetric expression. An 11-base-pair (bp) sequence required for AWC OFF expression has two activities: One region activates expression in both AWCs, and the overlapping NSY-7-binding site inhibits expression in AWC ON . Our results suggest that NSY-7 responds to transient embryonic signaling by repressing AWC OFF genes in AWC ON , thus acting as a transcriptional selector for a randomly specified neuronal identity.[Keywords: Homeodomain; olfactory development; transcriptional maintenance; transcriptional repression; activitydependent gene expression; cGMP-dependent protein kinase] Supplemental material is available at http://www.genesdev.org.

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“…These results emphasize that the DNA binding domain is highly conserved between Bombyx ANTP and Drosophila ANTP (Affolter et al 1990), while the putative domains for transcription activation and/or protein-protein interaction, if any, were altered during Diptera and Lepidoptera evolution. An alternative explanation for this variation could be that the Bombyx ANTP protein expressed in the larval middle silk glands is unique and different from that expressed in Bombyx embryos.…”

Section: Summary Figurementioning

confidence: 75%

**Developmental expression of the Bombyx Antennapedia homologue and homeotic changes in the Nc mutant**

et al. 1996

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Background: Abundant availability of homeotic mutants in Bombyx mori provides us with clues to study the body plan of the silkworm. Previous studies indicated that the E Ca /E Ca and E N /E N embryos, which reveal drastic morphological changes (Itikawa 1943, 1952) lack the homologue of abd-A and the homologues of abd-A and Ubx, respectively (Ueno et al. 1992). It will be interesting to characterize a mutant named Nc, the locus of which was mapped about 1.4 cm apart from the E loci (Itikawa 1944, 1952).

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DNA binding properties of the purified Antennapedia homeodomain.

Cited by 180 publications

References 36 publications

Kinetics of binding of Antp homeodomain to DNA analyzed by measurements of surface plasmon resonance

Kinetics of binding of Antp homeodomain to DNA analyzed by measurements of surface plasmon resonance

Transcriptional regulation and stabilization of left–right neuronal identity in C. elegans

**Developmental expression of the Bombyx Antennapedia homologue and homeotic changes in the Nc mutant**

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