DNA-Binding Specificity of the ERF/AP2 Domain of Arabidopsis DREBs, Transcription Factors Involved in Dehydration- and Cold-Inducible Gene Expression

Sakuma, Yoh; Liu, Qiang; Dubouzet, Joseph G.; Abe, Hiroshi; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko

doi:10.1006/bbrc.2001.6299

Cited by 1,641 publications

(1,843 citation statements)

References 42 publications

Supporting

Mentioning

1,736

Contrasting

Unclassified

Order By: Relevance

“…The members of this class of regulatory proteins all contain the conserved AP2/ERF domain, a 60-70 amino acid region that is thought to mediate DNA binding and protein-protein interactions (Jofuku et al 1994;Okamura et al 1997). The AP2/ERF family has been organized into Wve phylogenetically distinct subfamilies that diVer in the number of AP2/ERF domains, as well as the amino acid similarity between these domains (Sakuma et al 2002). Genes belonging to two of these subfamilies have been shown to enhance in vitro regeneration (Banno et al 2001;Boutilier et al 2002), while others play a role in related processes controlling meristem cell fate and organ development (Jofuku et al 1994;Elliot et al 1996;Chuck et al 1998;van der GraaV et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

Srinivasan

Liu

Heidmann

et al. 2006

Planta

145

View full text Add to dashboard Cite

Gain-of-function studies have shown that ectopic expression of the BABY BOOM (BBM) AP2/ ERF domain transcription factor is suYcient to induce spontaneous somatic embryogenesis in Arabidopsis (Arabidopsis thaliana (L.) Heynh) and Brassica napus (B. napus L.) seedlings. Here we examined the eVect of ectopic BBM expression on the development and regenerative capacity of tobacco (Nicotiana tabacum L.) through heterologous expression of Arabidopsis and B. napus BBM genes. 35S::BBM tobacco lines exhibited a number of the phenotypes previously observed in 35S::BBM Arabidopsis and B. napus transgenics, including callus formation, leaf rumpling, and sterility, but they did not undergo spontaneous somatic embryogenesis. 35S::BBM plants with severe ectopic expression phenotypes could not be assessed for enhanced regeneration at the seedling stage due to complete male and female sterility of the primary transformants, therefore fertile BBM ectopic expression lines with strong misexpression phenotypes were generated by expressing a steroid-inducible, posttranslationally controlled BBM fusion protein (BBM:GR) under the control of a 35S promoter. These lines exhibited spontaneous shoot and root formation, while somatic embryogenesis could be induced from in-vitro germinated seedling hypocotyls cultured on media supplemented with cytokinin. Together these results suggest that ectopic BBM expression in transgenic tobacco also activates cell proliferation pathways, but diVerences exist between Arabidopsis/B. napus and N. tabacum with respect to their competence to respond to the BBM signalling molecule.

show abstract

Section: Introductionmentioning

confidence: 99%

Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

Srinivasan

Liu

Heidmann

et al. 2006

Planta

145

View full text Add to dashboard Cite

show abstract

“…The PI value of about 3.8 of the C-terminal acidic domain indicates the presence of acidic domain. The structural analysis of AP2 domain indicated typical features of packing of α-helix nearly parallel to the three-stranded anti-parallel β-sheet, which are involved in DNA recognition as well binding specificity of the DREB1 proteins (Sakuma et al 2002;Allen et al 1998). Alignment analysis and domain comparison with proteins from different plant species therefore suggest that LlCBF is homologous to DREB1/CBF type of TFs.…”

Section: Discussionmentioning

confidence: 94%

“…The tertiary structure of LlCBF exhibits classic ERF fold consisting of three β-sheets connected by loops and a C-terminal α-helix packed approximate parallel to β-sheets. The two amino acids in β-sheet 14th valine and 19th glutamic acid play crucial role in the determination of DNAbinding specificity were found to be completely conserved (Liu et al 1998;Cao et al 2001;Sakuma et al 2002). The amino acid residues Arg6, Arg8, Trp10, Glu16, Arg18, Arg25 and Trp27 of AP2 domain that directly make contact with DNA for DNA-binding activity were also completely conserved except Trp10, which was substituted by amino acid serine (Allen et al 1998).…”

Section: Secondary and Three-dimensional Structural Analysesmentioning

confidence: 99%

“…DREB1/CBF like genes, belonging to the A-1 subgroup, are induced by low temperature stress but not by drought or high salt stresses, and therefore activates the expression of many cold stress-regulated genes. DREB2-like genes, belong to the A-2 subgroup, are mainly involved in regulation of genes responsive to osmotic stress (Sakuma et al 2002;Nakashima and Yamaguchi-Shinozaki 2006). Arabidopsis homologs of DREB1/CBF genes have been isolated and characterised in evolutionarily diverse plants with different levels of adaptation to cold stress such as, common wheat (Triticum aestivum L.), rice (Oryza sativa L.), rye (Secale cereale L.), maize (Zea mays L.), canola (Brassica napus), etc., and overexpression of DREB1/CBF genes in transgenic plants increases tolerance to drought, high salt, and freezing stresses (Dubouzet et al 2003;Shen et al 2003;Qin et al 2004;Zhang et al 2004a;Hong and Kim 2005;Benedict et al 2006;Zhao et al 2006;Chinnusamy et al 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cloning and characterization of cold, salt and drought inducible C-repeat binding factor gene from a highly cold adapted ecotype of Lepidium latifolium L.

Akhtar

Jaiswal

et al. 2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

The dehydration-responsive element-binding (DREB) protein/C-Repeat Binding Factors (CBFs) belongs to APETALA2 (AP2) family transcription factors that binds to DRE/CRT cis-element in cold-responsive (COR) genes and induce COR genes. CBFs have been isolated and characterized from evolutionarily diverse plant species. CBF pathway is conserved by CBF regulon and the size or the number and kind of target genes vary among freezing sensitive and tolerant plants. Hence, cloning of CBFs from highly freezing tolerant plants such as Lepidium latifolium L. will be useful in understanding the freezing tolerance of this species. In this study, LlCBF, a CBF1 family gene from L. Latifolium L., was cloned using RT-PCR and RACE-PCR. The full length mRNA of LlCBF is 948 bp with an open reading frame of 642 bp, encoding a protein of 213 amino acids with a molecular weight of 23.92 kDa and a theoretical isoelectric point of 4.80. Amino acid sequence analysis showed that LlCBF has an AP2 DNA binding domain, a potential CBF type nuclear localization signal (NLS) and C-terminal acidic domain. Semi-quantitative RT-PCR analysis of LlCBF revealed that this gene is up-regulated by high salt, dehydration and low temperature stresses. The investigation is therefore successful in cloning of a gene having strong homology with CBF transcription factors and responsive to low temperature, high salt and dehydration conditions.

show abstract

“…TFs bind specifically to their DNA-binding sites near target genes and then activate or repress gene transcription. The AP2/ERF superfamily is defined by the presence of the AP2 domain, which consists of 60-70 amino acids and is involved in DNA binding (1), and several AP2/ERF proteins have been suggested play roles in plant growth and development. According to Sakuma's classification, AP2/ERF proteins are classified into five subfamilies: AP2, RAV, DREB, ERF and others (1).…”

Section: Introductionmentioning

confidence: 99%

Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution

Jin¹,

Zhu²,

Peng³

et al. 2010

BMB Reports

View full text Add to dashboard Cite

In this study, we cloned the ERF-B3 subfamily transcription factor gene BnaERF-B3-hy15 from Brassica napus L. Huyou15. This 600 bp gene encodes a 199 amino acid classic ethylene responsive factor (ERF), which shown no binding or very weak binding GCC box-binding activity by the yeast one-hybrid assay. We used gene shuffling and the yeast one-hybrid system to obtain three mutated sequences that can bind to the GCC box. Sequence analysis indicated that two residues, Gly156 in the AP2 domain and Phe62 at the N-terminal domain were mutated to arginine and serine, respectively. Changes of Gly156 to arginine and Phe62 to serine increased the GCCbinding activity of BnaERF-B3-hy15 and the alter of Gly156 to arginine changed the AP2-domain structure of BnaERF-B3-hy15. [BMB reports 2010; 43(8): 567-572]

show abstract

DNA-Binding Specificity of the ERF/AP2 Domain of Arabidopsis DREBs, Transcription Factors Involved in Dehydration- and Cold-Inducible Gene Expression

Cited by 1,641 publications

References 42 publications

Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

Cloning and characterization of cold, salt and drought inducible C-repeat binding factor gene from a highly cold adapted ecotype of Lepidium latifolium L.

Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution

Contact Info

Product

Resources

About