DNA biosensor based on hybridization refractory mutation system approach for single mismatch detection

Ultrasensitive electrochemical genosensor for direct detection of specific RNA sequences derived from avian influenza viruses present in biological samples

“…The majority of reported electrochemical genosensors refer to the determination of DNA point mutations in buffer solutions or real samples (Joda et al, 2015;Sun et al, 2015;Yang et al, 2016;Rasheed et al, 2017).…”

Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay

“…Single-nucleotide polymorphisms (SNPs) are fundamental drivers of adaptation and disease origins in human cells. Therefore, SNPs can be used for patient stratification, facilitating personalized medicine approaches [ 1 ]. Detection of SNPs in human RNA is more challenging compared to DNA, as the RNA sequence is relative short, has a higher chemical and enzymatic instability, and the RNA active 3D folding decreases binding of the detection reagents [ 2 ].…”

“…As alternative approach, mismatch hybridization strategies for SNP detection has been developed in previous studies for longer RNA targets [ 1 , 8 ]. In situ PCR in combination with FISH has also been developed to enable simultaneous detection of point mutations and copy number variations at single-cell level in cancer tissue samples [ 9 ].…”

A sensitive DNA sensor based on an organic electrochemical transistor using a peptide nucleic acid-modified nanoporous gold gate electrode

“…Additionally, single nucleotide polymorphisms, a common form of genetic variations in the human genome, are important to disease diagnosis and detection of disease predisposition. 21,22 Therefore, the detection of a single-or two-base mismatch has been given much consideration by electrochemical, 23,24 optical, 25 and other methods. 26 However, designing highly sensitive and selective probes for a single-or two-base mismatch is not easy, and the fabrication process is complex.…”